Changes in misonidazole binding with hypoxic fraction in mouse tumors

The effect of hypobaric hypoxia on misonidazole binding in normal and tumour-bearing mice

“…Our results support the contention by Hirst et al (1985) that the increase in MISO binding observed in the tumours of mice which are rendered acutely anaemic is due to hypoxia rather than to haemodynamic changes induced by venesection and infusion of plasma. MISO binding may prove to be a useful way of assessing the effect in vivo of drugs that may kill cells within solid tumours by inducing hypoxia.…”

“…Since our method for inducing hypoxia does not cause vascular occlusion within the tumours, it is likely that the availability of MISO for binding to hypoxic cells was not altered. Hirst et al (1985) have suggested that, in some tumours, MISO binding may not correlate well with the radiobiological hypoxic fraction. One possible explanation for our results is that the CA NT tumour contains a very high proportion of hypoxic cells under normal conditions and that the reported radiobiological hypoxic fraction, which reflects clonogenic cells, is an underestimate of the total number of cells which are truly hypoxic.…”

Assessment of Hypoxia in Experimental Mice Tumours by [ 18 F] Fluoromisonidazole PET and pO 2 Electrode Measurements

“…N itroimidazoles were found to be reduced under hypoxic conditions and bound to cellular macromolecules (14,15), and this binding was shown to correlate with radiobiological hypoxia determined by a clonogenic cell survival assay (16). Binding of the hypoxia marker can be visualized by immunohistochemistry or autoradiography examination of biopsy tissue; e.g.…”

An enzyme-linked immunosorbent assay for hypoxia marker binding in tumours

“…The antisera to CCI-103F were known to respond strongly to the hexafluorinated side chain of CCI-103F (Raleigh et al, 1987) (Urtasun et al,1986) (Chapman, 1991). Smaller ranges of binding intensities of 2-3 were observed for transplanted murine tumours possessing the similar hypoxic fractions (Franko, 1986;Hirst et al, 1985).…”

“…While binding intensity in experimental tumours of a single type can be directly related to radiobiological hypoxic fraction or radiation response (Hirst et al, 1985;Li et al, 1991) . The value of the immunochemical approach in this context is that a single set of reagents provides for a rapid and inexpensive means of following changes in hypoxia marker binding (ELISA), which can be related to the fraction of cells binding the marker (immunohistochemical analysis) in the same biopsy samples (Cline et al, 1990).…”