Changes in mitochondrial activity evoked by cholecystokinin in isolated mouse pancreatic acinar cells

González, Antonio; Granados, María P.; Salido, Ginés M.; Pariente, José A.

doi:10.1016/s0898-6568(03)00067-6

Cited by 35 publications

(46 citation statements)

References 32 publications

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“…Using a · 60 oil immersion objective fluorescence, images of 256 · 256 pixels with a resolution of 0.287 mm/pixel were recorded every 4 s. Excitation light at 543 nm from a 25-mW helium-neon laser was employed. Emitted fluorescence was collected through a 605/32-nm band-pass filter, employing different photomultipliers as previously described (González et al, 2003). Neutral density filters had to be employed to reduce laser intensity to 1%-3% and diminish photobleaching.…”

Section: Determination Of Mitochondrial Free-ca 2 + Concentrationmentioning

confidence: 99%

“…Changes in c m were recorded using the dye TMRM as described previously (González et al, 2003). Cells were incubated during 30 min in the presence of 50 nM TMRM at 37°C.…”

Section: Mitochondrial Membrane Potential Determinationmentioning

confidence: 99%

“…After incubation, the extracellular medium was replaced by the fresh Na-HEPES buffer. This dye has been employed as a mitochondrial marker (González et al, 2003).…”

Section: Localization Of Mitochondriamentioning

confidence: 99%

“…Mitochondria are organelles with a high ability to sequester Ca 2 + when it is released from the ER. Accumulated Ca 2 + into mitochondria could mean a stimulatory signal that serves to modify the organelle activity (González et al, 2000;González et al, 2003). Ca 2 + released by ebselen could then accumulate into mitochondria.…”

Section: Effect Of Ebselen On Mitochondria Activitymentioning

confidence: 99%

“…In relation to a change in [Ca 2 + ] c , depolarization of c m occurs, which depends on Ca 2 + uptake by mitochondria (González et al, 2000;González et al, 2003). Thus, it could be possible that ebselen had an effect on c m .…”

Section: Effect Of Ebselen On Mitochondria Activitymentioning

confidence: 99%

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Ebselen Alters Mitochondrial Physiology and Reduces Viability of Rat Hippocampal Astrocytes

Santofimia‐Castaño

Salido

González

2013

DNA and Cell Biology

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The seleno-organic compound and radical scavenger ebselen (2-phenyl-1,2-benzisoselenazol-3(2H)-one) have been extensively employed as an anti-inflammatory and neuroprotective compound. However, its glutathione peroxidase activity at the expense of cellular thiols groups could underlie certain deleterious actions of the compound on cell physiology. In this study, we have analyzed the effect of ebselen on rat hippocampal astrocytes in culture. Cellular viability, the intracellular free-Ca 2 + concentration ([Ca 2 + ] c ), the mitochondrial freeCa 2 + concentration ([Ca 2 + ] m ), and mitochondrial membrane potential (c m ) were analyzed. The caspase-3 activity was also assayed. Our results show that cell viability was reduced by treatment of cells with ebselen, depending on the concentration employed. In the presence of ebselen, we observed an initial transient increase in [Ca 2 + ] c that was then followed by a progressive increase to an elevated plateau. We also observed a transient increase in [Ca 2 + ] m in the presence of ebselen that returned toward a value over the prestimulation level. The compound induced depolarization of c m and altered the permeability of the mitochondrial membrane. Additionally, a disruption of the mitochondrial network was observed. Finally, we did not detect changes in caspase-3 activation in response to ebselen treatment. Collectively, these data support the likelihood of ebselen, depending on the concentration employed, reduces viability of rat hippocampal astrocytes via its action on the mitochondrial activity. These may be early effects that do not involve caspase-3 activation. We conclude that, depending on the concentration used, ebselen might exert deleterious actions on astrocyte physiology that could compromise cell function.

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Section: Determination Of Mitochondrial Free-ca 2 + Concentrationmentioning

confidence: 99%

“…Changes in c m were recorded using the dye TMRM as described previously (González et al, 2003). Cells were incubated during 30 min in the presence of 50 nM TMRM at 37°C.…”

Section: Mitochondrial Membrane Potential Determinationmentioning

confidence: 99%

“…After incubation, the extracellular medium was replaced by the fresh Na-HEPES buffer. This dye has been employed as a mitochondrial marker (González et al, 2003).…”

Section: Localization Of Mitochondriamentioning

confidence: 99%

Section: Effect Of Ebselen On Mitochondria Activitymentioning

confidence: 99%

Section: Effect Of Ebselen On Mitochondria Activitymentioning

confidence: 99%

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Ebselen Alters Mitochondrial Physiology and Reduces Viability of Rat Hippocampal Astrocytes

Santofimia‐Castaño

Salido

González

2013

DNA and Cell Biology

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Single‐cell sequencing of entorhinal cortex reveals widespread disruption of neuropeptide networks in Alzheimer's disease

Larsen

2023

Alzheimer's & Dementia

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INTRODUCTIONAbnormalities of neuropeptides (NPs) that play important roles in modulating neuronal activities are commonly observed in Alzheimer's disease (AD). We hypothesize that NP network disruption is widespread in AD brains.METHODSSingle‐cell transcriptomic data from the entorhinal cortex (EC) were used to investigate the NP network disruption in AD. Bulk RNA‐sequencing data generated from the temporal cortex by independent groups and machine learning were employed to identify key NPs involved in AD. The relationship between aging and AD‐associated NP (ADNP) expression was studied using GTEx data.RESULTSThe proportion of cells expressing NPs but not their receptors decreased significantly in AD. Neurons expressing higher level and greater diversity of NPs were disproportionately absent in AD. Increased age coincides with decreased ADNP expression in the hippocampus.DISCUSSIONNP network disruption is widespread in AD EC. Neurons expressing more NPs may be selectively vulnerable to AD. Decreased expression of NPs participates in early AD pathogenesis. We predict that the NP network can be harnessed for treatment and/or early diagnosis of AD.

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Nodular hidradenocarcinoma over the parotid gland: A pathologic presentation

2006

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Background. Nodular hidradenocarcinoma (NHAC), an eccrine carcinoma, has been reported in the dermatology and pathology literature, but few references have been made in the otolaryngology literature even though the head and neck is a common site of occurrence.Methods. A case report of a 37-year-old Hispanic man with a right-sided neck mass diagnosed preoperatively as a parotid mass by imaging and fine-needle aspiration is presented. After presentation at our multidisciplinary tumor board, excision of the mass was undertaken.Results. Final pathology reveled a NHAC, which is presented in our report.Conclusion. NHAC is an aggressive malignant tumor that is often misdiagnosed preoperatively and that must be treated with aggressive multimodality therapy for increased survival. Head Neck 29: 193-197, 2007 Keywords: nodular hidradenocarcinoma; eccrine tumor; malignant skin tumor; skin cancer Nodular hidradenocarcinoma (NHAC) is a malignant cell eccrine tumor, often with clear cell morphology, which has been reported less than 60 times in the literature. It has been rarely reported in the otolaryngology literature, though the head and neck is a common site of occurrence. The tumor has been identified in the literature under many names including clear-cell hidradenocarcinoma, malignant clear-cell hidradenoma, solidcystic adenocarcinoma, malignant acrospiroma, malignant clear-cell myoepithelioma, and clearcell eccrine carcinoma. 1 NHAC tumors usually develop as malignant tumors, though there have been reports of malignant degeneration of the benign counterpart, hidradenomas. 2 We present the case of a patient with what was believed to be a parotid neoplasm by preoperative clinical examination that turned out to be a hidradenocarcinoma. CASE REPORTA 37-year-old otherwise healthy Hispanic man presented to our tertiary care hospital complaining of a painless right neck mass that the patient said had been present for approximately 3 months. On physical examination the patient had a 4 cm 3 2 cm right parotid mass that appeared to be connected to the skin though there were no skin changes. Facial nerve function was House Brackmann I/VI bilaterally. The patient was sent for fine-needle aspiration of the neck mass, which showed cellular clusters and sheets of monomorphous epithelial cells with low nuclear:cytoplasm

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Changes in mitochondrial activity evoked by cholecystokinin in isolated mouse pancreatic acinar cells

Cited by 35 publications

References 32 publications

Ebselen Alters Mitochondrial Physiology and Reduces Viability of Rat Hippocampal Astrocytes

Ebselen Alters Mitochondrial Physiology and Reduces Viability of Rat Hippocampal Astrocytes

Single‐cell sequencing of entorhinal cortex reveals widespread disruption of neuropeptide networks in Alzheimer's disease

Nodular hidradenocarcinoma over the parotid gland: A pathologic presentation

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