Changes in mitochondrial enzymatic activities of monocytes during prolonged hypobaric hypoxia and influence of antioxidants: A randomized controlled study

Merz, Tobias M.; Hefti, Jacqueline Renée Pichler; Hefti, Urs; Huber, Andreas; Jakob, Stephan M.; Takala, Jukka; Djafarzadeh, Siamak

doi:10.1179/1351000215y.0000000007

Cited by 3 publications

(2 citation statements)

References 45 publications

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“…66 as well. Moreover, as CS is considered a marker of mitochondrial mass (Civitarese et al 2007, Merz et al 2015, its decrease suggests that one of the main causes for the reduced respiratory chain enzyme mRNA expression might be the reduction in the amount of mitochondria. Collectively, these data further support the association between obesity and T2DM and impaired mitochondrial activity in subcutaneous white adipose tissue (Rieusset 2015, Brands et al 2012, Dahlman et al 2006, Gianotti et al 2008.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Very-Low-Calorie Diet on Mitochondrial Dysfunction in Subcutaneous Adipose Tissue and Peripheral Monocytes of Obese Subjects with Type 2 Diabetes Mellitus

Urbanová¹,

Mráz²,

Ďurovcová³

et al. 2017

Physiol Res

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Mitochondrial dysfunction is a potentially important player in the development of insulin resistance and type 2 diabetes mellitus (T2DM). We investigated the changes of mRNA expression of genes encoding main enzymatic complexes of mitochondrial respiratory chain in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM) of 11 subjects with simple obesity (OB), 16 obese patients with T2DM and 17 healthy lean subjects (C) before and after very low-calorie diet (VLCD) using quantitative real time PCR. At baseline in SCAT, both T2DM and OB group had decreased mRNA expression of all investigated mitochondrial genes with the exception of 2 complex I (NDUFA 12) and complex IV (COX 4/1) enzymes in OB subjects. In contrast, in PM only the expression of complex I enzymes NDUFA 12 and MT-ND5 was reduced in both T2DM and OB subjects along with decreased expression of citrate synthase (CS) in T2DM group. Additionally, T2DM subjects showed reduced activity of pyruvate dehydrogenase and complex IV in peripheral blood elements. VLCD further decreased mRNA expression of CS and complex I (NT-ND5) and II (SDHA) enzymes in SCAT and complex IV (COX4/1) and ATP synthase in PM of T2DM group, while increasing the activity of complex IV in their peripheral blood elements. We conclude that impaired mitochondrial biogenesis and decreased activity of respiratory chain enzymatic complexes was present in SCAT and PM of obese and diabetic patients. VLCD improved metabolic parameters and ameliorated mitochondrial oxidative function in peripheral blood elements of T2DM subjects but had only minor and inconsistent effect on mitochondrial gene mRNA expression in SCAT and PM.

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Section: Discussionmentioning

confidence: 99%

The Effect of Very-Low-Calorie Diet on Mitochondrial Dysfunction in Subcutaneous Adipose Tissue and Peripheral Monocytes of Obese Subjects with Type 2 Diabetes Mellitus

Urbanová¹,

Mráz²,

Ďurovcová³

et al. 2017

Physiol Res

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“…Esse aumento poderia ser uma resposta das células neurais frente a uma infecção que estaria afetando a função mitocondrial, e, portanto, um aumento na quantidade dessa organela seria uma tentativa de manter as atividades celulares normais. Um aumento no número mitocondrial em resposta a situações de dano na atividade mitocondrial já foi relatado (Merz et al, 2015), dano este que poderia também estar associado com a proteína NSs de OROV, por exemplo, já que esta poderia possuir atividade parecida com a proteína NSs de um vírus pertencente a ordem Bunyavirales, a mesma de OROV, e que co-localiza com a mitocôndria e leva ao rompimento da membrana mitocondrial, com consequente alteração do potencial de membrana desta (Barnwal et al, 2016).…”

Section: Discussão 5) Discussãounclassified

Estudo da infecção do sistema nervoso central pelo vírus Oropouche em um modelo de fatias de cérebro humano adulto

Almeida¹

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Almeida, Glaucia Maria. Study of central nervous system infection by the Oropouche vírus in adult human brain slice cultures. Ribeirão Preto, 2020. The Oropouche virus (OROV), of the family Peribunyaviridae, genus Orthobunyavirus, is a causative agent of arbovirus called "Oropouche fever". This virus has the significant spreading capacity, and in recent years has been found in nonendemic region, especially in South American countries, including Brazil. Although some clinical evidences in alterations in central nervous system, as meningitis, has been associated with OROV infection, few studies involving OROV neuropathogenesis have been studied, and all knowledge obtained were showed from neonatal murine models. This difficulty in studying OROV neuropathogenesis in the human central nervous system is related to the lack of models that maintain the complexity of human brain. Therefore, in this study, we evaluated the OROV neuroinfection using adult human brain slices cultures. We showed for the first time that OROV infects human neural cells and caused tissue damage within 48 h post infection, such as loss of cell viability and TNF-α release, a proinflammatory cytokine. We showed, by immunofluorescence, microglia cells were the cell most susceptible to OROV infection, but that it retains the ability to infect neurons. This is the first study to report OROV infection in adult human brain model and should become useful in neuropathogenesis studies related to this emerging pathogen.

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Cardiac and placental mitochondrial characterization in a rabbit model of intrauterine growth restriction

Guitart‐Mampel

Gonzalez-Tendero

Niñerola

et al. 2018

Biochimica et Biophysica Acta (BBA) - General Subjects

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Changes in mitochondrial enzymatic activities of monocytes during prolonged hypobaric hypoxia and influence of antioxidants: A randomized controlled study

Cited by 3 publications

References 45 publications

The Effect of Very-Low-Calorie Diet on Mitochondrial Dysfunction in Subcutaneous Adipose Tissue and Peripheral Monocytes of Obese Subjects with Type 2 Diabetes Mellitus

The Effect of Very-Low-Calorie Diet on Mitochondrial Dysfunction in Subcutaneous Adipose Tissue and Peripheral Monocytes of Obese Subjects with Type 2 Diabetes Mellitus

Estudo da infecção do sistema nervoso central pelo vírus Oropouche em um modelo de fatias de cérebro humano adulto

Cardiac and placental mitochondrial characterization in a rabbit model of intrauterine growth restriction

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