Changes in Monoamine Oxidase Activity in Rat Brain During Alloxan Diabetes

Abstract

The effect of alloxan diabetes on the activity of monoamine oxidase was studied in three regions of the rat brain at various time intervals after the onset of diabetes. It was observed that monoamine oxidase activity was decreased at early time intervals after diabetes, followed by a recovery in all three regions of the brain. A reversal of the effect was observed with insulin administration to the diabetic rats.

Role of 5-Hydroxytryptamine in the Regulation of Brain Neuropeptides in Normal and Diabetic Rat

“…and 5-HT, may alter the central level of one or more of these amines. Mayanil et al [23] reported that MAO activ ity in various rat brain regions was decreased significantly during the early onset of dia betes induced by alloxan. This observation supports our data and may explain in part the significant elevation in brain 5-HT levels 15 days postinduction of diabetes.…”

“…Demonstration that fluo xetine, a highly specific inhibitor of 5-HT uptake [14,35], potentiates morphine analge sia [33] in footshock [26], hot plate [23] and tail flick [21] tests for analgesia in rats arc among the pharmacological evidence that en hancement of serotonincrgic function in creases morphine analgesia. Moreover, the analgesic effect produced by microinjection of opiate into the midbrain periaqueductal grey (PAG) is abolished by 5-HT antagonists, suggesting a possible involvement of both 5-HT and opiate [36].…”

Changes in Phosphofructokinase and Pyruvate Kinase in Rat Brain Regions during Alloxan-Induced Diabetes

“…Changes in several brain enzymes related to glucose, pyruvate, ATP and catecholamine metabolism during alloxan diabetes have re cently been reported [6][7][8][9]. Phosphofructo kinase (ATP:Z)-hexose-6-phosphate 1-phos photransferase, EC 2.7.1.11) and pyruvate kinase (ATP:pyruvate phosphotransferase, Diabetes leads to several neurological complications in man and animals, including disturbances in sensory and motor systems [1], Severe derangement in cerebral energy metabolism under diabetic conditions is noted by several authors [2][3][4], A decreased conversion of glucose to its metabolism and a reduction in glucose with an increase in ke tone body uptake by the diabetic rat brain have been reported [2,3].…”

Influence of Alloxan Diabetes and Insulin Treatment on the Activity of Alanine Aminotransferase in Rat Brain Regions, Liver and Heart

“…Thereafter, the animals were divided into two groups, one being diabetic and the other with continued treatment of insulin, as described earlier [9]. The animals were sacrificed after 3,8, 15 and 22 days of the onset of diabetes.…”

“…The homogenization and subcellular fractiona tion of the tissues were performed by the method of Hess and Brand [11], as described earlier [9], The solu ble and the particulate fractions (12,000-^ pellet), after removing nuclear, cell debris, etc., were used for the enzyme estimation.…”