Public Wageningen Food & Biobased Research | 5 Netherlands), where the punnets were randomized per ripeness level, labelled and stored at 4°C and 80% RH. On days 1, 5 and 9 after harvest and labelling, respective samples were placed at 20°C and 80% RH for 6 hours prior to the measurements. Volatile analysis To determine volatile organic compound (VOC) production of the strawberries, the strawberries in a punnet were carefully transferred into an amber Duran GLS 80 wide mouth laboratory bottle of 2000 mL (DWK Life Sciences GmbH, Mainz, Germany) with septa mounted in the lids. After flushing with clean and filtered air for 1.5 min, the jars were closed, moved and after 1.2 h of headspace accumulation sampled using a PTR Qi-ToF-MS 8000 instrument (Ionicon Analytik GmbH, Innsbruck, Austria). The PTR-ToF-MS had a drift voltage of 900V at 60°C and 3.8 mbar, resulting in an E/N of 121 Td. Sampling flow rate was 60 mL/min and the mass range was 20-512 m/z. Samples were taken from the jars by direct injection into the PTR-ToF-MS drift tube through a heated (110°C) peek inlet connected to a syringe needle. PTR-ToF-MS data was analysed using the program PTRwid (Holzinger, 2015), after which noise reduction was achieved by averaging over 20 consecutive and stable ToF