Changes in Plasma Adenosine Concentrations during Normal Pregnancy

Yoneyama, Yoshio; Suzuki, Shunji; Sawa, Rintaro; Takeuchi, Tsutomu T.; Kobayashi, Hajime; Takei, Rintaro; Kiyokawa, Yasuko; Otsubo, Yasuo; Hayashi, Zuisei; Araki, Tsutomu

doi:10.1159/000010313

Cited by 16 publications

(17 citation statements)

References 16 publications

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“…In our study, adenylate energy charge and ATP/ADP ratio in the metabolic pool of platelets were significantly increased in patients with preeclampsia, while total adenine nucleotides level was decreased. Such results suggest increased activation of platelets in our patients with preeclampsia [29,30]. Further confirmation of increased activation of platelets in patients with preeclampsia comes from observed decrease of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) levels in their platelets [31,32].…”

Section: Discussionsupporting

confidence: 64%

Changes of platelets’ function in preeclampsia

2011

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AbstractIncreased aggregation of platelets during preeclampsia was shown in several studies, yet several others reported no change. The aim of our study was to investigate platelet aggregation in a group of patients suffering from preeclampsia. In a cross-sectional study blood samples were taken from 89 hospitalized patients in the third trimester of pregnancy: 38 were suffering from mild to moderate preeclampsia and 51 patients were without preeclampsia. From the blood samples platelet aggregation, secretion of adenine nucleotides from platelets, concentration of energy-rich adenine compounds and levels of cyclic adenosine-mono-phosphate and cyclic guanosine mono-phosphate in platelets were measured. In the patients with preeclampsia, the adenosine diphosphate threshold for biphasic aggregation [odds ratio (OR):.75; 95% Confidence Interval (CI): 0.55–1.02; p<0.05], total adenine nucleotides concentration in the metabolic pool of platelets (OR:0.99; CI: 0.62–1.57; p<0.01) and cyclic adenosine-mono-phosphate (OR:0.81; CI: 0.57, 1.14; p<0.05) and cyclic guanosine mono-phosphate (OR:.78; CI: 0.55–1.09; p<0.05) levels in platelets were decreased in comparison with the control group, while adenylate energy charge in the metabolic pool of platelets (OR: >100.00; CI: 0.00->100.00; p<0.05) and secretion of adenosine triphosphate (OR:.13; CI: 0.00–14.26; p<0.05) and adenosine diphosphate (OR:.77; CI: 0.08–36.79; p<0.05) were increased. The results of our study show increased activation and aggregation of platelets in pregnant females with preeclampsia.

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Section: Discussionsupporting

confidence: 64%

Changes of platelets’ function in preeclampsia

2011

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“…6,66 The elevated adenosine level may be explained by platelet activation (releasing ATP and ADP), increments in plasma activity of 5′-nucleotidases (CD73), or decreases in adenosine deaminase (ADA) activity during pregnancy. 66,67 Also, ATP may be hydrolyzed faster during pregnancy because the ATP hydrolyzing enzymes CD39 and alkaline phosphatase are highly expressed in the placenta. 12,68 These pregnancy adaptations suggest that extracellular ATP levels need to be regulated tightly during pregnancy.…”

Section: Atp and Adenosine During Normal Pregnancymentioning

confidence: 99%

“…66 During pregnancy, many maternal physiological adaptations are necessary to accommodate the developing fetus. For instance, blood volume and cardiac output rise by 50%, whereas blood pressure slightly decreases.…”

Section: Atp and Adenosine During Normal Pregnancymentioning

confidence: 99%

Danger Signals From ATP and Adenosine in Pregnancy and Preeclampsia

et al. 2014

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“…The concentrations of adenosine needed for the protective effect are clearly higher than those encountered in peripheral plasma, which have been reported to range between 0.1 and 1 μM in healthy individuals [88,[92][93][94]. However, plasma adenosine concentrations are higher in the pulmonary circulation [74] and may approach 10 μM in disease such as sepsis [57].…”

Section: Discussionmentioning

confidence: 97%

Adenosine protects against suicidal erythrocyte death

Niemoeller

Bentzen

Lang

et al. 2007

Pflugers Arch - Eur J Physiol

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Suicidal death of erythrocytes or eryptosis is characterized by cell shrinkage and cell membrane scrambling leading to phosphatidylserine exposure at the erythrocyte surface. The cell membrane scrambling is triggered by an increase in cytosolic Ca(2+) activity and activation of protein kinase C (PKC). Phosphatidylserine exposure fosters adherence of affected erythrocytes to the vascular wall. Thus, microcirculation in ischemic tissues may be impaired by the appearance of eryptotic erythrocytes. Ischemia leads to release of adenosine, which in most tissues leads to vasodilation and protects against cell injury. The present experiments explored whether adenosine influences mechanisms underlying eryptosis. Erythrocyte phosphatidylserine exposure was estimated from annexin V binding, cell volume from forward scatter and cytosolic Ca(2+) activity from Fluo3 fluorescence. Glucose depletion (for 24 or 48 h) significantly increased annexin binding and decreased forward scatter, effects partially reversed by adenosine. The protective effect of adenosine reached statistical significance (s.d.) at> =30 microM. Low Cl(-) solution (Cl(-) exchanged by gluconate for 24 h) similarly increased annexin binding and decreased forward scatter, effects again reversed by adenosine (s.d. at > or =10 and 30 microM, respectively). Similarly, phosphatase inhibitor okadaic acid (OA, 1 microM) and PKC activator phorbol 12-myristate-13-acetate (PMA, 3 microM) significantly enhanced annexin binding and decreased forward scatter. Adenosine significantly blunted the effects of OA and PMA on annexin V binding (s.d. at > or =30 and 10 microM, respectively) and the effect of OA on forward scatter (s.d. at > or =10 microM). In conclusion, adenosine inhibits eryptosis by a mechanism presumably effective downstream of PKC. The effect may participate in the maintenance of microcirculation in ischemic tissue.

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Changes in Plasma Adenosine Concentrations during Normal Pregnancy

Cited by 16 publications

References 16 publications

Changes of platelets’ function in preeclampsia

Changes of platelets’ function in preeclampsia

Danger Signals From ATP and Adenosine in Pregnancy and Preeclampsia

Adenosine protects against suicidal erythrocyte death

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