1998
DOI: 10.5511/plantbiotechnology.15.17
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Changes in Protein Pattern during Stress-induction of Carrot (Daucus carota L.) Somatic Embryogenesis.

Abstract: To clarify the molecular biological aspects occurring during the induction of somatic embryogenesis, the stress-induced changes in protein pattern and the proteins associated with somatic embryogenesis induced by stress in carrot (Daucus carota L.) were investigated. In two dimensional polyacrylamide gel electrophoresis (2D-PAGE), 6 spots with molecular weight of 63-65 kilo Dalton (KDa) showing pI ranging from 6.0-6.5, and 2 spots of molecular weight 45 KDa with a pI of 5.0 were detected in the extracts of api… Show more

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Cited by 9 publications
(16 citation statements)
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“…Many types of embryo-specific genes, including LEA-class genes, are expressed in these cells before the formation of morphologically differentiated somatic embryos (Wild et al 1988, Kiyosue et al 1991, 1993a, 1993b, Lin et al 1996, Tachikawa et al 1998. For this reason, it has been postulated that embryogenic cells are already programmed for early-and late-stage embryogenesis at the molecular level, and it is difficult to efficiently isolate early embryonic genes using these cells (Kiyosue et al 1991(Kiyosue et al , 1993a(Kiyosue et al , 1993b.…”
Section: Discussionmentioning
confidence: 99%
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“…Many types of embryo-specific genes, including LEA-class genes, are expressed in these cells before the formation of morphologically differentiated somatic embryos (Wild et al 1988, Kiyosue et al 1991, 1993a, 1993b, Lin et al 1996, Tachikawa et al 1998. For this reason, it has been postulated that embryogenic cells are already programmed for early-and late-stage embryogenesis at the molecular level, and it is difficult to efficiently isolate early embryonic genes using these cells (Kiyosue et al 1991(Kiyosue et al , 1993a(Kiyosue et al , 1993b.…”
Section: Discussionmentioning
confidence: 99%
“…The plant materials collected were true leaves from 4-week-old plants, immature seeds from different plants at 3,15,21,27,36,45, and 54 days after flowering (DAF), and mature dry seeds. Embryogenic cells (EC) and somatic embryos were induced as described (Tachikawa et al 1998), and non-embryogenic cells (NC) that had lost the ability to form somatic embryos were used as negative controls for the acquisition of embryogenic competence (Satoh et al 1986). Murashige and Skoog (MS) media (Murashige and Skoog 1962) with or without 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) were used as the basal media for this experiment.…”
Section: Plant Materialsmentioning
confidence: 99%
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“…Treatments such as explant wounding, or exposure to medium containing 2,4-D, may result in the rapid proliferation of cells that become undifferentiated (or de-differentiated) and lose their original polarity [219] or attain a hyperpolarized state [59]. This interference with polar auxin transport abolishes auxin gradients, which subsequently halts the programmed organogenesis and tropic responses [195,196]. This involves the erasure of existing transcriptional and translational patterns in order to redirect the developmental program of cells [42] and is marked by an increase in endogenous auxin levels [143], which along with exogenous auxin application, is a critical factor during the induction and expression of SE [81].…”
Section: Pgrsmentioning
confidence: 99%