Changes in root canal microbiota during the development of rat periapical lesions

Tani‐Ishii, Nobuyuki; Wang, C.-Y.; Tanner, A. C. R.; Stashenko, Philip

doi:10.1111/j.1399-302x.1994.tb00048.x

Cited by 77 publications

(51 citation statements)

References 22 publications

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“…After determination of the important role of bacteria in the pathogenesis of pulp and periapical lesions (1,2), elimination of infection from the root canal system became the objective of endodontic treatment of teeth with necrotic pulp and periapical lesions (3,4).…”

Section: Introductionmentioning

confidence: 99%

Bacterial profile in primary teeth with necrotic pulp and periapical lesions

et al. 2006

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The objective of this study was to evaluate the bacterial profile in root canals of human primary teeth with necrotic pulp and periapical lesions using bacterial culture. A total of 20 primary teeth with necrotic pulp and radiographically visible radiolucent areas in the region of the bone furcation and/or the periapical region were selected. After crown access, 4 sterile absorbent paper points were introduced sequentially into the root canal for collection of material. After 30 s, the paper points were removed and placed in a test tube containing reduced transport fluid (RTF) and were sent for microbiological evaluation. Anaerobic microorganisms were found in 100% of the samples, black-pigmented bacilli in 30%, aerobic microorganisms in 60%, streptococci in 85%, Gram-negative aerobic rods in 15% and staphylococci were not quantified. Mutans streptococci were found in 6 root canals (30%), 5 canals with Streptococcus mutans and 1 canal with Streptococcus mutans and Streptococcus sobrinus. It was concluded that in root canals of human primary teeth with necrotic pulp and periapical lesions, the infection is polymicrobial with predominance of anaerobic microorganisms.

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Section: Introductionmentioning

confidence: 99%

Bacterial profile in primary teeth with necrotic pulp and periapical lesions

et al. 2006

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“…Depending on the virulence and number of microorganisms present in RCs, acute or chronic inflammation may be established in the periapical region. 1,2 In infected primary teeth, lesions normally develop in the furcation area instead of around the tooth apex, which could be related not only to the high incidence of accessory furcation canals 3 but also to infections with certain bacteria at these sites. However, few studies have been performed using molecular methods to identify the presence of anaeroDeclaration of Interests: The authors certify that they have no commercial or associative interest that represents a conflict of interest in connection with the manuscript.…”

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confidence: 99%

An investigation of the presence of specific anaerobic species in necrotic primary teeth

et al. 2013

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Different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. The aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (PCs) and root canals (RCs) of infected primary teeth using PCR methods. Paired PC and RC samples were collected from 15 subjects and analyzed by PCR for the presence of Filifactor alocis, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella tannerae, Tanerella forsythia, Treponema denticola, and Treponema socranskii. The frequency of each species was determined in the PC and RC of each case. The species most frequently detected in PCs were P. nigrescens (86.7%), P. gingivalis (73.3%), and F. alocis (73.3%). Of the PC samples, 13.3% contained P. micra and T. denticola, and 6.7% contained T. forsythia. The species most frequently detected in RCs were P. gingivalis (100%) and P. nigrescens (93.3%). P. tannerae, P. micra, and T. denticola were found in 40% of the RC samples; T. forsythia was found in 26.7% of the RC samples. The "red complex", which comprises P. gingivalis, T. denticola, and T. forsythia, was not found in the PC of any tooth but was found in 30% of the RC samples. The detection of P. nigrescens in the PC was statistically associated with the presence of P. nigrescens in the RC (p = 0.04). The results suggest high heterogeneity among the samples, even among those from the same subject.

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“…Sundqvist (16) demonstrated that bacteria could only be isolated from intact traumatized teeth with associated periapical lesions and not from necrotic teeth without lesions. The nature of the rootcanal microbiota associated with periapical-lesion development has been reviewed extensively in humans (17)(18)(19)(20) and in nonhuman primates (21,22). These studies indicated that there is a strong correlation between infection of the root canal and development of apical periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Clinical and Radiographic Evaluation of Root-canal Obturation with Obtura II

Tani‐Ishii

Teranaka

2003

Journal of Endodontics

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This study evaluated clinical and radiographic healing of 236 root-canal treatments in 131 cases obturated with the Obtura II system. One operator performed all canal preparation and obturation with sealer. A standardized apical-coronal preparation technique instrumented all canals. Clinical symptoms, periodontal condition, and radiographic findings were evaluated at 3, 6, and 12 months. Radiographs taken immediately postobturation were compared to recall radiographs. The level of the final root filling was classified as short (more than 2 mm short of the apex), flush (within 2 mm), or over (beyond) in 12.7%, 81.4%, and 5.9% of cases, respectively. More than 96% of cases were treated successfully by the Obtura II system. Where roots were filled flush, over, or short, lesions healed in 97%, 93%, and 93% of cases, respectively, with no significant differences (p < 0.05). Root filling excess had no impact on the healing process.Thermoplasticized, injectable, obturation techniques have been developed and available for several years. Heated gutta-percha techniques (low-temperature, high-temperature) have been shown to produce a significantly better quality root-canal obturation than that produced by the lateral-condensation technique (1-3). Especially the high-temperature technique, Obtura II system (Texceed Co., Fenton, MO) has been found to be significantly superior to lateral-condensation methods and has demonstrated the best adaptation to the three-dimensional root-canal system (1).In vitro studies by Weller and Koch (4) (1994) have shown that the Obtura II technique demonstrated the best adaptation to prepared root canals, and the Obtura II system also is commonly used for back-filling. Johnson and Bond (5) suggested that it might be clinically acceptable to backfill canals up to 10 mm in a single increment using sealer and the Obtura II system. The high temperature generated in the root canal can be dissipated through the root surface and periodontal ligament. Molyvdas et al. (6) showed histologically the inflammation reaction in periapical tissue after injection of warm gutta-percha in beagle dogs. However, it is generally accepted that a temperature rise of approximately 10°C above normal body temperature is most critical (7). Weller and Koch (8) (1995) measured the radicular temperatures produced when gutta-percha was heated to 160°C, 185°C, and 200°C and injected into the root canal by Obtura II. The results indicated that the rise in temperature on the root surface was below the level of 10°C and did not cause damage to the periodontal ligament. These in vivo and in vitro studies have shown that Obtura II has a good adaptation to the canal wall and causes no periodontal tissue injury. However, there have been no studies reporting a clinical patient case study of the Obtura II system. The purpose of this study was to evaluate the clinical and radiographic healing of 131 teeth (236 canals) of 87 patients to estimate the relationship of the root-filling level in relation to the root apex, and assess outcomes...

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Changes in root canal microbiota during the development of rat periapical lesions

Cited by 77 publications

References 22 publications

Bacterial profile in primary teeth with necrotic pulp and periapical lesions

Bacterial profile in primary teeth with necrotic pulp and periapical lesions

An investigation of the presence of specific anaerobic species in necrotic primary teeth

Clinical and Radiographic Evaluation of Root-canal Obturation with Obtura II

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