Changes in S1P1 and S1P2 expression during embryonal development and primitive endoderm differentiation of F9 cells

Abstract

Sphingosine 1-phosphate (S1P) is a ligand for S1P family receptors (S1P 1 -S1P 5 ).Of these receptors, S1P 1 , S1P 2 , and S1P 3 are ubiquitously expressed in adult mice, while S1P 4 and S1P 5 are tissue-specific. However, little is known of their expression during embryonal development. We performed Northern blot analyses in mouse embryonal tissue and found that such expression is developmentally regulated. We also examined the expression of these receptors during primitive endoderm (PrE) differentiation of mouse F9 embryonal carcinoma (EC) cells, a well-known in vitro endoderm differentiation system. S1P 2 mRNA was abundantly expressed in F9 EC cells, but little S1P 1 and no S1P 3 , S1P 4 , or S1P 5 mRNA was detectable. However, S1P 1 mRNA expression was induced during EC-to-PrE differentiation. Studies using small interference RNA of S1P 1 indicated that increased S1P 1 expression is required for PrE differentiation. Thus, S1P 1 may play an important function in PrE differentiation that is not substituted for by S1P 2 .

Systemic distribution, subcellular localization and differential expression of sphingosine-1-phosphate receptors in benign and malignant human tissues

“…A total of five subtypes of S1PRs (S1PR1-5) have been identified since the first S1PR was discovered by Hla T et al (1990). During the past two decades, S1P and its specific receptors were found to be involved in a wide range of pathophysiological processes (Maceyka et al, 2012) in multiple organ systems that include: the central nervous system, immune system (Soliven et al, 2011; Garris et al, 2014), cardiovascular system (Schmouder et al, 2012), and in embryonic development (Kondo et al, 2014; Hiraga et al, 2006). Further, emerging evidence indicates that S1P/S1PRs play significant roles in the proliferation, progression, survival and therapeutic response to treatment in cancer (Pyne et al, 2010; Kunkel et al, 2013; Pyne et al, 2013).…”

Cell-Culture–Dependent Change of Ca2+ Response of Rat Aortic Myocytes to Sphingosine-1-Phosphate

“…On the other hand, enhanced expression of S1P 2 was observed in primary cultured rat hepatocytes depending on cell-cycle transition from G0 to G1, and thus S1P 2 expression was up-regulated during proliferating status (33). F9 embryonic carcinoma cells showed down-regulation for S1P 2 expression when differentiated (35). In addition, myogenic differentiation in C2C12 myoblasts was accompanied with downregulation of S1P 2 (36).…”