Changes in Saccharide Metabolism Induced by Infection of Camellia Sinensis by Exobasidium Vexans

Effect of blister blight disease caused by Exobasidium on tea quality

“…Among the 10 up-regulated sugars, sucrose and maltose belonging to disaccharide accounted for 50.40 % of the total sugars, 7 kinds of monosaccharides including d -xylose, d -arabinose, d -mannose, d -glucuronic acid, glucose, d -galactose and d -fructose only accounted for 15.49 %, and the other 1 trisaccharide (raffinose) accounted for 4.64 % in CB, indicating that monosaccharide and disaccharide were the most abundant differential soluble sugars in diseased tea, which may contribute to the enhanced sweet taste of diseased tea. However, Pius et al (1998) found that sucrose and glucose contents were significantly decreased in the blistered lesions, and the content of fructose was remarkably increased during the initiation of sporulation and remained constant up to the end of sporulation in non-blistered and blistered regions. The decrease of the sugar accumulation in diseased tea leaves may be due to the increase in the utilization or consumption rate by the pathogen as material basis during the infection process.…”

Acid phosphatase and invertase activities of <i>Aspergillus niger</i>

“…The acid invertase activity was inversely proportional to the starch content and closely related to the changes in saccharose and glucose content (Pius et al 1998). The signifi cantly enhanced enzyme activity began with the onset of sporulation and reached a maximum at the stage of maximum basidiospore release, when it was nearly ten times higher than in the control (Pius et al 1998). Although the localization of invertase is not yet clear (Scholes et al 1994), its increasing activity during sporulation may be caused by a fungal enzyme as previously proposed (Xiu et al 1993).…”

“…Reviewing nutrient relationships in biotrophic infections emphasized that acid invertase is the most studied relevant enzyme in infected leaves, and it is of central interest in view of its probable role in hydrolysis of sucrose before sugar uptake by fungi (Schaeffer et al 1995). The acid invertase activity was inversely proportional to the starch content and closely related to the changes in saccharose and glucose content (Pius et al 1998). The signifi cantly enhanced enzyme activity began with the onset of sporulation and reached a maximum at the stage of maximum basidiospore release, when it was nearly ten times higher than in the control (Pius et al 1998).…”