Fresh noodle is one of the staple foods in many Asian countries that have been consumed for thousands of years (Chen et al., 2019). Nowadays, fresh noodles have been favored increasingly by consumers due to its freshness, good taste, and unique flavor (Tuersuntuoheti, Wang, Zheng, et al., 2019). As consumers are becoming more health conscious, a new trend in consumer demand for nutritionally fortified noodles rich in dietary fibers and phytochemicals has risen (Bai et al., 2017). Qingke barley is a cultivar of hulless barley and consumed as a staple food in Qinghai-Tibet Plateau of China. It has been proved that Qingke barley is rich in bioactive compounds including dietary fiber (β-glucan) and phytochemicals (Yong et al., 2015), which could prevent and mitigate various diseases, including obesity, high blood pressure, type 2 diabetes, chronic heart disease, and colon cancer (Sullivan et al., 2013). Therefore, Qingke barley fresh noodles (QBFN) is favored by more consumers. However, because of the high initial (total plate count) TPC and high moisture content, the shelf life of QBFN is relatively short. It deteriorates very easily at room temperature (Sullivan et al., 2013). To promote its industrialization and meet the consumer demand, the shelf life of QBFN should be extended by using appropriate preservation methods. Microwave (MW) irradiation has been used for recent centuries, it reaches the bactericidal effect by combining the nonthermal and thermal sterilizing in a very short time (Fung & Cunningham, 1980).