Changes in sialylation of low‐density lipoprotein in coronary artery disease

Abstract

Reduction of LDL sialylation may correlate with coronary artery disease (CAD), but the details of this modification and its effect on CAD are not well studied. This study was aimed to show desialylation of LDL and to reveal more details of this modification. Blood samples were collected from 16 patients with CAD and 25 healthy individuals. Serum sialic acid was determined. LDL was extracted from all samples, and the interaction of the extracted materials with lectins (MAA, SNA, and DSA) was studied using the lectin blotting method. Serum total sialic acid (TSA) concentrations in CAD patients and healthy individuals were 71.9 6 2.66 and 60.76 6 2.34 mg/dL, respectively, and the difference between the two groups was statistically significant (p ,0.001). The intensity of interaction of extracted LDL with SNA and MAA lectins was lower in CAD patients compared to that in normal subjects (p ,0.001). The intensity of interaction of LDL with DSA was higher in CAD (p ,0.001). There was a reverse correlation between TSA and intensity of LDL interaction with SNA and MAA in both groups, but in the case of DSA this correlation was direct and positive. These findings indicated an increase in desialylation of LDL in CAD. It was concluded that LDL was subjected to glycosylation changes in CAD and that there was a positive correlation between TSA and the desialylated form of LDL. This modification may contribute to the pathogenesis of CAD.

The Role ofAnethum graveolensL. (Dill) in the Management of Diabetes

“…Glycation reaction leads to alteration in the structure and function of biomolecules particularly proteins. AGE formation is known as a key element in diabetes complications [41–46]. …”

Explorations in a galaxy of sialic acids: a review of sensing horizons, motivated by emerging biomedical and nutritional relevance

“…265 A cohort study on 18 429 men and 19 414 women of age 59.5 ± 6.5 years along with 40 years of follow-up revealed that the elevated blood Sia concentration (>median 700 mg L −1 ) and pulse pressure (>median 70 mm Hg) could both independently predict the risk of cardiovascular incident for patients of cardiovascular diseases (P < 0.0001). 266 268 Furthermore, total Sia concentration in the serum and saliva of periodontitis patients increased in the comorbidity of cardiovascular diseases. 269 The total Sia concentration in the serum can also statistically identify (P < 0.0001) the induction of carotid atherosclerosis in humans caused by plaque deposition in the brain arteries.…”

“…267 The coronary artery disease also caused desialylation of low-density lipoprotein and increased the total Sia concentration in serum of the patients (mean 71.91 (68.33–75.60) mg dL −1 ; P < 0.001) compared to the healthy control (60.76 (57.04–65.92) mg dL −1 ). 268 Furthermore, total Sia concentration in the serum and saliva of periodontitis patients increased in the comorbidity of cardiovascular diseases. 269 The total Sia concentration in the serum can also statistically identify ( P < 0.0001) the induction of carotid atherosclerosis in humans caused by plaque deposition in the brain arteries.…”