Changes in SNAI1 and VIM gene expression in Caco2 cells after co-cultivation with bacteria from colorectal cancer biopsies

Abstract

The development of distant metastases is the final stage in the solid cancer progression and it is responsible for the majority of cancer-related deaths. Epithelial-mesenchymal transition (EMT) is involved in cancer progression and metastasis. In the present study, we used different types of intracellular bacteria isolated from colorectal cancer biopsies to examine their effect on the expression of SNAI1 and VIM genes in Caco2 cell line. SNAI1 gene expression was significantly decreased after co-cultivation of Caco2 cells with Pseudomonas aeruginosa, Proteus vulgaris, Proteus mirabilis, Enterococcus faecalis, Klebsiella pneumoniae and Bacillus cereus, respectively (p<0.05). We observed more than 2-fold increase in VIM gene expression within Caco2 cells after co-cultivation with Proteus vulgaris. On the other hand, VIM gene expression decreased by half after co-cultivation with Pseudomonas aeruginosa and Proteus mirabilis (p<0.05). Our data suggest that bacteria presented in colorectal carcinoma tissues may cause changes in gene expression of EMT-associated genes. Further research is needed to find out whether bacteria are capable to support EMT and cancer progression.

3D Disease Modelling of Hard and Soft Cancer Using PHA-Based Scaffolds

“…The porous 3D PHA scaffolds were prepared by solvent casting–particulate leaching technique [ 67 ], which is a standard method to produce polymer-based scaffolds. The NaCl sieved through a 300 µm sieve ensured that the 3D PHA scaffolds have a well-connected variable pore size.…”

“…The EMT programme is orchestrated through transcription factors like Snail, Slug and Zeb1 that are responsible for the gain of mesenchymal properties [ 66 ]. Snail is linked to tumour progression and invasiveness due to its ability to alter the expression of the Vimentin gene ( vim) [ 67 ]. The latter is one of the mesenchymal markers responsible for maintaining cell shape, cytoplasm integrity and stabilizing cytoskeletal interactions and is found downstream of the Snail gene.…”

Investigate the stemness of adult adipose‐derived stromal cells based on single‐cell RNA‐sequencing

“…Proteins encoded by CFL1, S100A6, ACTB, VIM, PFN1, ANXA2, RAN , and TPM4 have been found to be involved in the transport of actin–cofilin complex from the cytoplasm to nucleus, cell cycle process and differentiation, cell structure integrity and intercellular signal transfer, cytoskeleton stabilization and cytoplasmic integrity, regulation of cell growth, control of DNA synthesis, and regulation of microtubule polymerization during mitosis. All of these factors are critical in cell proliferation and differentiation (Filipek & Leśniak, 2020; Gibbs et al, 2019; Guo et al, 2013; Huang et al, 2020; Luo et al, 2021; Song et al, 2020; Wachsmannova et al, 2019; Z. L. Yang et al, 2013; C. Zhang et al, 2020). At the same time, the KEGG signal route was shown to be engaged in the Rap1 signal pathway, the Hippo signal pathway, focal adhesion, and other molecular signal pathways, all of which are significant in the activity of ADSCs stem cells.…”

“…TPM4 has the ability to govern tissue repair and regeneration (You et al, 2014). In tumor cells, the expression of ACTB, VIM, and PFN1 was increased (Guo et al, 2013; Huang et al, 2020; Wachsmannova et al, 2019). These evidence implying that Cluster 3 had the most stemness.…”

“…At the same time, pseudo-time may be used to examine cell subsets or key genes of important differentiation nodes in the process of cell differentiation or development. This approach is now widely used to recreate the developmental pathway and differentiation (Filipek & Leśniak, 2020;Gibbs et al, 2019;Guo et al, 2013;Huang et al, 2020;Luo et al, 2021;Song et al, 2020;Wachsmannova et al, 2019;Z. L. Yang et al, 2013;C.…”