Changes in spleen histology in response to antigenic stimulation in the snapping turtle, <i>Chelydra serpentine</i>

Borysenko, Myrin

doi:10.1002/jmor.1051490206

Cited by 19 publications

(9 citation statements)

References 44 publications

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“…Concerning the morphology of the spleen of the desert tortoise Testudo graeca, our results revealed that it has red and white pulps that come in agreement with that mentioned by Borysenko [46], in the snapping turtle (Chelydra serpentine).…”

Section: Discussionsupporting

confidence: 77%

Morphology of Blood Cells, Liver and Spleen of the Desert Tortoise (Testudo graeca)

Kassab¹,

Shousha²,

Fargani³

2009

Open Anat J

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Abstract:The morphological characteristics of peripheral blood cells, the anatomical structure of the liver and spleen were examined in desert tortoise. Forty healthy adult Testudo graeca tortoises, 20 females and 20 males were selected for this study. The tortoises were given thorough examination, and a blood sample was collected from the heart by cardiocentesis. The Wright staining method was used for classification of the blood cells. Seven different types of blood cells were determined: erythrocytes, heterophils, eosinophils, basophils, lymphocytes, monocytes and thrombocytes. Mature erythrocytes of captive Testudo graeca were nucleated ellipsoidal cells. Erythrocytes measurements for males were (length 18.76 ± 2.81 m width 9.52 ± 2.23 m) and for females were (length 19.19 ± 2.90 m width 9.84 ± 2.50 m). The heterophil contain large, eosinophilic, ovoid, cytoplasmic granules with eccentric nucleus. The eosinophil is distinguished by its round, eosinophilic cytoplasmic granules and the nucleus is round to oval, single or bi-lobed, and eccentrically placed within the cytoplasm. The basophil was easily identified by its deeply stained purple, large, round granules that remained tightly adhered to the centrally located nucleus. The lymphocyte contained a small amount of blue staining cytoplasm and a round nucleus with a fine reticular pattern. The monocyte contains a large amount of light bluegray, finely granular or vacuolated cytoplasm, and an oval or indented nucleus. Thrombocytes were oval-shaped cells contained round, densely stained nucleus. The liver composed of two lobes, right and left which were connected with a narrow band of connective tissue. The parenchyma of the tortoise liver is seen to be composed of intersinusoidal cords of hepatocytes. The spleen grossly appeared as round or oval structure. The parenchyma (splenic pulp) formed of lymphoid tissue and is of two distinct types; white and red pulps.Overall, this study provides identification of the morphological characteristics of different peripheral blood cells of the tortoise Testudo graeca species as well as the anatomy of the liver and spleen, as a reference for future hematological studies of this species and may be used as a basis for comparison in clinical cases.

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Section: Discussionsupporting

confidence: 77%

Morphology of Blood Cells, Liver and Spleen of the Desert Tortoise (Testudo graeca)

Kassab¹,

Shousha²,

Fargani³

2009

Open Anat J

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“…Moreover, in the turtle spleen, BORYSENKO (1976) observed that large pyroninophilic cells formed lymphoid foci in the PALS. He did not, however, regard these as germinal centers.…”

Section: Discussionmentioning

confidence: 94%

Primary formation of germinal centers in the chick spleen after injection with sheep red blood cells.

Fukuta

Nishida

Mochizuki

1980

Arch. Histol. Jap., Arch. Histol. Jpn

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“…In reptiles, the splenic histology shows considerable variation. In the turtles Chelydra serpentina (Borysenko, 1976) and Mauremys caspica (Zapata et al, 1981a1, the pattern is very similar to that described in Bufo calamita and Xenopus laevis; but evidence for a marginal zone is lacking in the lizards, Calotes versicolor (Kanakambika and Muthukkaruppan, 19731, and Lacerta hispanica (Zapata, unpublished observations).…”

Section: Discussionmentioning

confidence: 62%

Non‐lymphoid cells of the anuran spleen: An ultrastructural study in the natterjack, Bufo calamita

et al. 1983

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The present study is concerned with the ultrastructure of the spleen in the natterjack, Bufo calamita (Anura, Bufonidae), with special emphasis on the structure and function of the non-lymphoid elements occurring in the red and white pulp. The organ consists of two clearly distinguishable areas, the white and the red pulp, separated by a prominent marginal zone. Thus, the pattern of lymphocytic arrangement in the spleen of Bufo calamita corresponds to a follicular model and is similar to that reported in the primitive anurans of the Pipidae family, such as Xenopus laevis. The white pulp presents a reticular network consisting of two different cell types and free cells, such as lymphocytes, plasma cells, and macrophages. The red pulp is formed by cell cords, where reticular cells and fibers, macrophages and lymphocytes occur, and blood sinuses which sometimes contain developing erythroid elements. Colloidal carbon particles injected via the lymph sac are trapped exclusively by free macrophages in the red pulp which then move through the marginal zone to the white pulp. Giant, ramified, non-phagocytic cells appear in both white and red pulp. They have been functionally related with the trapping of antigen-antibody complexes on their surface, and a possible dendritic significance is discussed on the basis of their morphologic characteristics.

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Changes in spleen histology in response to antigenic stimulation in the snapping turtle, Chelydra serpentine

Abstract: Proliferative and migratory changes of lymphoid cells in the spleen were observed in turtles stimulated with KLH and maintained at 30°C.

Cited by 19 publications

References 44 publications

Morphology of Blood Cells, Liver and Spleen of the Desert Tortoise (Testudo graeca)

Morphology of Blood Cells, Liver and Spleen of the Desert Tortoise (Testudo graeca)

Primary formation of germinal centers in the chick spleen after injection with sheep red blood cells.

Non‐lymphoid cells of the anuran spleen: An ultrastructural study in the natterjack, Bufo calamita

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