Changes in structure and composition of lymphocyte nuclei during mitogenic stimulation

Setterfield, G.; Hall, R. H.; Bladon, Trevor; Little, Judy E.; Kaplan, Joseph

doi:10.1016/s0022-5320(83)80014-8

Cited by 81 publications

(60 citation statements)

References 36 publications

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“…The results are also consistent with observations on nuclear reorganization in mitogen-stimulated lymphocytes, in which increases in nuclear volume, enlargement of nucleoli and the development of extensive interchromatin regions are paralleled by increases in the number and density of nuclear pores (Maul et al 1972), the influx and quantity of non-histone nuclear proteins (Setterfield et al 1983(Setterfield et al , 1985, and the immunofluorescence labeling pattern and intensity of antibody PI1 (Setterfield et al 1985;Chaly et al 1988).…”

Section: Discussionsupporting

confidence: 88%

“…Goldstein 1974;Harris 1974;Ringertz and Savage 1976;Bonner 1978;Scheer et al 1983;Setterfield et al 1983;Woodcock et al t984;Gurdon 1986), but only recently has it become feasible to modulate nucleocyAbbreviations: WGA wheat germ agglutinin; GlcNAc N-acetylglucosamme Ojjprint requests to: R. Benavente toplasmic exchange processes of macromolecules experimentally. It is now clear that wheat germ agglutinin (WGA), a lectin binding to N-acetylglucosamine (GlcNAc) sugar residues, binds to nuclear pore complexes and interferes with the signal-dependent translocation of karyophilic proteins into isolated nuclei in vitro (Finlay et al 1987 ;Newmeyer and Forbes 1988).…”

Section: Introductionmentioning

confidence: 99%

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Functional role of newly formed pore complexes in postmitotic nuclear reorganization

et al. 1989

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Abstract. Many nuclear proteins are released into the cytoplasm at prometaphase and are transported back into the daughter nuclei at the end of mitosis. To determine the role of this reentry in nuclear remodelling during early interphase, we experimentally manipulated nuclear protein uptake in dividing cells. Recently we and others have shown that signal-dependent, pore complex-mediated uptake of nuclear protein is blocked in living cells on microinjection of the lectin wheat germ agglutinin (WGA), or of antibodies such as PI1 that are directed against WGA-binding pore complex glycoproteins. In the present study, we microinjected mitotic PtK z cells with WGA or antibody PIt and followed nuclear reorganization of the daughter cells by immunofluorescence and electron microscopy. The inhibitory effect on nuclear protein uptake was monitored by co-injection of the karyophilic protein nucleoplasmin. When injected by itself early in mitosis, nucleoplasmin became sequestered into the daughter nuclei as they entered telophase. In contrast, nucleoplasmin was excluded from the daughter nuclei in the presence of WGA or antibody PI1 . Although PtK z cells with blocked nuclear protein uptake completed cytokinesis, their nuclei showed a telophaselike organization characterized by highly condensed chromatin surrounded by a nuclear envelope containing a few pore complexes. These findings suggest that pore complexes become functional as early as telophase, in close coincidence with nuclear envelope reformation. They further indicate that the extensive structural rearrangement of the nucleus during the telophase-G1 transition is dependent on the influx of karyophilic proteins from the cytoplasm through the pore complexes, and is not due solely to chromosome-associated components.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Functional role of newly formed pore complexes in postmitotic nuclear reorganization

et al. 1989

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“…Journal of Cell Science 117 (25) Concomitantly, the nuclei enlarge dramatically as much of the facultative chromatin decondenses (Setterfield et al, 1983). This transition allows direct comparisons to be made between the highly repressed state and active, cycling nuclei.…”

Section: Nuclear Macroh2a In Quiescent Lymphocytes Is Concentrated Inmentioning

confidence: 99%

Dynamic relocation of epigenetic chromatin markers reveals an active role of constitutive heterochromatin in the transition from proliferation to quiescence

Grigoryev

Nikitina

Pehrson

et al. 2004

Journal of Cell Science

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“…From the ultrastructural standpoint, it is clear that the nuclear matrix is a dynamic structure that changes during the cell cycle (Riley and Keller, 1978;Setterfield et al, 1983;Chaly et al, 1984). However, we have observed that the proteins Ml-M4 show neither selective synthesis nor degradation in quiescent and proliferating cells.…”

Section: Discussionmentioning

confidence: 59%

Synthesis and stability of nuclear matrix proteins in resting and serum‐stimulated swiss 3T3 cells

Milavetz

Edwards

1986

Journal Cellular Physiology

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The major [35S]methionine-radiolabeled nuclear matrix proteins of mouse 3T3 cells were isolated, and most of these were found to be similar in molecular weight, charge, and protease fingerprint to the nuclear matrix proteins of African green monkey kidney cells, which are found tightly bound to simian virus 40 chromosomes. These nuclear matrix proteins were found to be synthesized in quiescent and serum-stimulated cells and to be turned over slowly during pulse-chase experiments. In contrast, a 70-Kd (kilodalton) neutral protein identified as lamin a was found to be turned over rapidly, producing a 68-Kd protein with a similar isoelectric point. In addition, we observed a decrease in the amounts of two chromatin-bound matrix proteins and a relative increase in lamin a following labeling in the presence of 2 micrograms/ml actinomycin D. However, these effects do not appear to be a result of inhibition of transcription, since they were not observed with other inhibitors (alpha-amanitin and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole).

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Changes in structure and composition of lymphocyte nuclei during mitogenic stimulation

Cited by 81 publications

References 36 publications

Functional role of newly formed pore complexes in postmitotic nuclear reorganization

Functional role of newly formed pore complexes in postmitotic nuclear reorganization

Dynamic relocation of epigenetic chromatin markers reveals an active role of constitutive heterochromatin in the transition from proliferation to quiescence

Synthesis and stability of nuclear matrix proteins in resting and serum‐stimulated swiss 3T3 cells

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