Changes in Tear Proteomic Profile in Ocular Diseases

Winiarczyk, Mateusz; Biela, Katarzyna; Michalak, Katarzyna; Winiarczyk, Dagmara; Mackiewicz, Jerzy

doi:10.3390/ijerph192013341

Cited by 8 publications

(4 citation statements)

References 83 publications

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“…A number of proteomic studies on AMD have already been conducted, with sometimes conflicting results, yet there are some patterns in the identified proteins and the metabolic pathways that they are involved in that support our current knowledge of the disease's etiology [4][5][6][7]. In our previous studies, we focused on the tear film proteome, providing interesting findings [8][9][10]. In this study, we aim to characterize the serum proteome of nAMD patients and discuss the potential clinical value of the obtained results.…”

Section: Introductionmentioning

confidence: 88%

Oxidative Stress, Persistent Inflammation and Blood Coagulation Alterations in Serum Proteome of Patients with Neovascular Age-Related Macular Degeneration

Winiarczyk,

Thiede,

Utheim

et al. 2024

Life

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Neovascular age-related macular degeneration (AMD) is a major cause of irreversible blindness in elderly populations in developed countries. AMD’s etiopathology is multifactorial, with strong environmental and genetic components, but the exact molecular pathomechanisms underlying the disease are still unknown. In this study, we analyzed blood serum collected from 74 neovascular AMD patients and 58 healthy controls to identify proteins that may serve as potential biomarkers and expand our knowledge about the etiopathogenesis of the disease. The study revealed 17 differentially expressed proteins—11 up-regulated and 6 down-regulated—in neovascular AMD, which are involved in the biological processes previously linked with the disease—oxidative stress and persistent inflammation, impaired cellular transport, lipid metabolism and blood coagulation. In conclusion, the differences in the expressions of the proteins identified in this study may contribute to our understanding of the mechanisms underlying AMD and possibly serve in future as promising biomarkers.

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Section: Introductionmentioning

confidence: 88%

Oxidative Stress, Persistent Inflammation and Blood Coagulation Alterations in Serum Proteome of Patients with Neovascular Age-Related Macular Degeneration

Winiarczyk,

Thiede,

Utheim

et al. 2024

Life

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“…It is worth noting that distinct sampling methods carry specific advantages and limitations. Thus, comparing different approaches for collecting tear fluid and assessing their suitability could prove valuable when addressing specific research inquiries [39,43,103]. Current research suggests that potential tear biomarkers for AD include lipocalin-1, lysozyme-C, lacritin, eIF4E, Aβ38, Aβ40, Aβ42, Aβ1-42, T-tau and P-tau [67][68][69][70][71][72].…”

Section: Future Perspectivesmentioning

confidence: 99%

Tear Biomarkers and Alzheimer’s Disease

Kaštelan,

Braš,

Pjevač

et al. 2023

IJMS

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Alzheimer’s disease (AD) is an age-related progressive neurodegenerative brain disorder that represents the most common type of dementia. It poses a significant diagnostic challenge that requires timely recognition and treatment. Currently, there is no effective therapy for AD; however, certain medications may slow down its progression. The discovery of AD biomarkers, namely, magnetic resonance imaging, positron emission tomography and cerebrospinal fluid molecules (amyloid-β and tau) has advanced our understanding of this disease and has been crucial for identifying early neuropathologic changes prior to clinical changes and cognitive decline. The close interrelationship between the eye and the brain suggests that tears could be an interesting source of biomarkers for AD; however, studies in this area are limited. The identification of biomarkers in tears will enable the development of cost-effective, non-invasive methods of screening, diagnosis and disease monitoring. In order to use tears as a standard method for early and non-invasive diagnosis of AD, future studies need to be conducted on a larger scale.

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“…Other approaches include imaging modalities such as anterior segment optical coherence tomography and in vivo confocal microscopy [14]. Genomic and metagenomic approaches [15], as well as tear proteomic analysis [16], show promise for improving the diagnosis and monitoring of infectious keratitis.…”

Section: Introductionmentioning

confidence: 99%

Bacterial and Fungal Keratitis in a Tertiary Care Hospital from Romania

Bălășoiu,

Zlatian

et al. 2024

Microorganisms

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Infectious keratitis is a significant global problem that can lead to corneal blindness and visual impairments. This study aimed to investigate the etiology of infectious bacterial and fungal keratitis, identify the causative pathogens and their antimicrobial resistance patterns, and analyze the risk factors associated with the development of infectious keratitis. The study was observational and retrospective, involving 226 eyes from 223 patients presented at the Ophthalmology Clinic of the County Clinical Emergency Hospital of Craiova, Romania. The inclusion criteria included corneal ulceration/abscess/infiltrate present on slit-lamp examination and positive microbiological sampling for bacteria or fungi. The study found that the most common causes of infectious keratitis were coagulase-negative staphylococci (35.40%), Staphylococcus aureus (11.06%), and Pseudomonas aeruginosa (14.16%). The Gram-positive bacteria showed high resistance rates to penicillin, moderate rates to gentamycin and clindamycin, and low resistance to chinolones. The Gram-negative bacteria were highly resistant to ampicillin and amoxicillin–clavulanic acid, while third-generation cephalosporins, quinolones, and carbapenems were effective. Systemic antibiotics, such as vancomycine, piperacillin–tazobactam, amikacin, and ceftazidime, show promise against keratitis with low resistance rates, whereas carbapenems and topical aminoglycosides had higher resistance, leaving moxifloxacin as a potential topical option for Gram-positive bacteria and Pseudomonas aeruginosa, albeit with resistance concerns for Klebsiella spp. Although fungal keratitis was rare, Fusarium spp. and Candida albicans were the leading fungal pathogens, with incidences of 2.65% and 2.21%, respectively. Candida albicans was broadly susceptible to most antifungals, while Fusarium solani, Curvularia lunata, and Alternaria alternata exhibited resistance to many antifungals. Amphotericin B and caspofungin can be used as systemic antifungals in fungal keratitis. The study also identified risk factors for keratitis such as ocular trauma (65.92%, OR: 2.5), contact lens wear (11.94%, OR: 1.8), and corneal scarring/leukoma (10.17%, OR: 1.6). Keratitis was more frequent in individuals over 60 years old. The findings of this study have implications for the development of effective diagnostic, therapeutic, and preventive strategies for infectious keratitis.

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Changes in Tear Proteomic Profile in Ocular Diseases

Cited by 8 publications

References 83 publications

Oxidative Stress, Persistent Inflammation and Blood Coagulation Alterations in Serum Proteome of Patients with Neovascular Age-Related Macular Degeneration

Oxidative Stress, Persistent Inflammation and Blood Coagulation Alterations in Serum Proteome of Patients with Neovascular Age-Related Macular Degeneration

Tear Biomarkers and Alzheimer’s Disease

Bacterial and Fungal Keratitis in a Tertiary Care Hospital from Romania

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