Changes in the Activity of Lipases in the Presence of Synthetic and Natural Polymers

Savina, A. A.; Garnashevich, Lilia S.; Zaitsev, Ilia S.; Tsarkova, Marina S.; Zaitsev, S. Yu.

doi:10.3103/s0027131419060130

Cited by 3 publications

(4 citation statements)

References 7 publications

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“…values were carried out using a BPA-1P device by the method of the maximum pressure measurements in a bubble (with a surface lifetime of 0.5 s to 10 s) [39][40][41].…”

Section: Measurements Of the Current Surface Tension (St)mentioning

confidence: 99%

“…The general principle of the device operation (based on the maximum pressure in the bubble method [39][40][41]) is described below [39]. The air from the compressor enters the capillary, which is lowered into the test liquid.…”

Section: Measurements Of the Current Surface Tension (St)mentioning

confidence: 99%

“…This method of the maximal pressure measurement "in the buoyant bubble" allowed us to obtain the dependences of DST values vs. time (i.e., in the form of "tensiograms" [41]). All measurements were carried out at 20°C.…”

Section: Figurementioning

confidence: 99%

“…All measurements were carried out at 20°C. The equilibrium surface tension (eST) values were obtained by a calculation from the "ST vs. time" isotherms using the ADSA program [39][40][41]. The particle sizes in the obtained colloidal solutions were determined by the method of dynamic light scattering with the use of "90Plus/BI-MAS Particle Size Analyzer" device (Brookhaven Instruments Corporation, USA) [25].…”

Section: Figurementioning

confidence: 99%

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Interaction of Lipase with Lipid Model Systems

Zaitsev

Milaeva

2020

J. Res. Updates Polym. Sci.

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The aim of this work was to study the interaction of lipases (as an important biopolymer) with models of biomembranes based on the phospholipid and cholesterol. Lipases (triacylglycerolacyl hydrolases) are widely distributed enzymes and well-known by their hydrolytic activity. The study of the lipase interactions with lipid vesicles in aqueous dispersions is of fundamental and practical interest. The pure phosphatidylcholine from egg yolk (ePC) and cholesterol (Chol) were obtained from Sigma-Aldrich. Lipase was obtained from hog pancreas. Measurements of the current and equilibrium surface tension (ST and eST) values were carried out using a BPA-1P device and ADSA program. The particle sizes in the prepared colloidal solutions were determined by the method of dynamic light scattering. An addition of lipase led to some decrease both, of ST and eST for the samples of ePC:Chol (in the ratios from19:1 to 1:1). The mean particle diameter (MPD) and effective particle diameter (EPD) values for the samples of ePC:Chol changed drastically by lipase addition. The EPD/MPD ratios increased from 1.7 to 2.0, from 1.8 to 2.6, from 2.3 to 6.5, from 1.5 to 2.9 for the samples of ePC:Chol at the ratios of 19:1, 14:1, 9:1, 7:1, respectively by lipase concentration increase. This general tendency can be explained by strong interaction of lipase with lipid membrane that leads to the formation of the mixed particles ePC:Chol:lipase with more narrow particle size distribution as compared to the initial EPD/MPD ratio (for the ePC:Chol mixture without lipase).

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“…values were carried out using a BPA-1P device by the method of the maximum pressure measurements in a bubble (with a surface lifetime of 0.5 s to 10 s) [39][40][41].…”

Section: Measurements Of the Current Surface Tension (St)mentioning

confidence: 99%

Section: Measurements Of the Current Surface Tension (St)mentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Interaction of Lipase with Lipid Model Systems

Zaitsev

Milaeva

2020

J. Res. Updates Polym. Sci.

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Different strategies for the lipase immobilization on the chitosan based supports and their applications

Rafiee

Rezaee

2021

International Journal of Biological Macromolecules

103

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Functional supramolecular systems: design and applications

et al. 2021

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The interest in functional supramolecular systems for the design of innovative materials and technologies, able to fundamentally change the world, is growing at a high pace. The huge array of publications that appeared in recent years in the global literature calls for systematization of the structural trends inherent in the formation of these systems revealed at different molecular platforms and practically useful properties they exhibit. The attention is concentrated on the topics related to functional supramolecular systems that are actively explored in institutes and universities of Russia in the last 10–15 years, such as the chemistry of host–guest complexes, crystal engineering, self-assembly and self-organization in solutions and at interfaces, biomimetics and molecular machines and devices. The bibliography includes 1714 references.

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Changes in the Activity of Lipases in the Presence of Synthetic and Natural Polymers

Cited by 3 publications

References 7 publications

Interaction of Lipase with Lipid Model Systems

Interaction of Lipase with Lipid Model Systems

Different strategies for the lipase immobilization on the chitosan based supports and their applications

Functional supramolecular systems: design and applications

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