Changes in the antioxidative metabolism induced by Apple chlorotic leaf spot virus infection in peach [Prunus persica (L.) Batsch]

García-Ibarra, A.; Clemente‐Moreno, María José; Barba‐Espín, Gregorio; Díaz‐Vivancos, Pedro; Rubio, M.; Dicenta, F.; Martínez‐Gómez, Pedro; Hernández, José Antonio

doi:10.1016/j.envexpbot.2010.10.004

Cited by 7 publications

(5 citation statements)

References 37 publications

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“…In addition, the increase in number of infected plants between the first and the second cycle suggests that ACLSV needs time to multiply and infect rootstocks. This slow multiplication rate in comparison with other viruses such as PPV or PNRSV has been described in other works on ACLSV (Bernhard et al 1969;Cambra et al 1986;Dosba et al 1986;García-Ibarra et al 2011). Furthermore, the low success of grafting of infected plants in our study is consistent with the previous results of Desvignes and Boyé (1988), who described a decrease of more than 50 % in graft compatibility in different Prunus rootstocks after inoculation with ACLSV.…”

Section: Discussionsupporting

confidence: 94%

Evaluation of apricot (Prunus armeniaca L.) resistance to Apple chlorotic leaf spot virus in controlled greenhouse conditions

et al. 2012

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Apple chlorotic leaf spot virus (ACLSV) seems to be the causal agent of apricot viruela disease. This disease has become an important problem for apricot production in Spain, mainly affecting the 'Búlida' cultivar, although no information is available about the behaviour of other cultivars with regards to ACLSV. In this study, the behaviour of 29 apricot cultivars against ACLSV (Apr 62 isolate) was evaluated under controlled conditions in an insect-proof greenhouse. Three different rootstocks, 'GF305' peach, 'Real Fino' apricot and 'Adesoto' plum, were first inoculated by grafting ACLSV-infected bark and were later grafted with the apricot cultivar to be evaluated. Apricot cultivars were evaluated during three cycles of study. ACLSV was asymptomatic on the leaves of all cultivars and rootstocks, so level of susceptibility or resistance was determined by virus detection through RT-PCR. 'GF305' rootstock showed a greater susceptibility level than 'Real Fino' and 'Adesoto'. Most of the cultivars were susceptible to ACLSV with different levels of susceptibility, and only 'Bergeron' and 'Mauricio' were resistant.

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Section: Discussionsupporting

confidence: 94%

Evaluation of apricot (Prunus armeniaca L.) resistance to Apple chlorotic leaf spot virus in controlled greenhouse conditions

et al. 2012

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“…Peach GF305 is used commonly for grafting different peach varieties as well as being a model plant and rootstock for the study of different viruses affecting fruit species such as PPV or ACLSV (apple chlorotic leaf spot virus) (Hernández et al 2004;Díaz-Vivancos et al 2006;García-Ibarra et al 2011). It has been used to study the effect of PPV infection on antioxidative metabolism at the subcellular level and on the differential expression of proteins in the apoplastic space, using a proteomic approach (Hernández et al 2004;Díaz-Vivancos et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Implication of peroxidase activity in development of healthy and PPV-infected micropropagated GF305 peach plants

2011

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In this work, we describe the micropropagation of PPV-infected GF305 peach plants for their use as a continuous source of PPV inoculums. We observed that PPV was maintained in each sub-culture as well as in the complete micropropagation cycle, including shoot proliferation, rooting and acclimatization to ex vitro conditions. We assayed the addition of fructose and ferulic acid to the multiplication media, to improve the quality and vigour of shoots. This increased the growth of explants and this effect was correlated with a reduction in POX activity, suggesting the possible participation of POX in the growth regulation of micropropagated GF305 shoots. In general, PPV-infected explants did not show evident Sharka symptoms under in vitro conditions, but some explants showed the typical interveinal chlorosis produced by PPV. However, all the PPV-acclimated plants developed normal Sharka symptoms. The data shows that shoot cultures can be used as a reservoir of PPV inoculums as well as to study different metabolic response of healthy and PPV-infected plantlets. In addition, this strategy could serve as a secure and constant source of both healthy and virus-infected plants for other molecular and biological studies.

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“…Characterization of the GSTU10-10 isoenzyme revealed that it has an antioxidant catalytic function by acting as a hydroperoxidase and has a very low K m for GSH suggesting that GSTU10-10 is able to perform efficient catalysis under conditions where GSH concentrations are low, e.g., during oxidative stress (Skopelitou et al, 2015). A long term systemic infection of peach by Apple chlorotic leaf spot virus (ACLSV), the causal agent of “viruela” disease was investigated focusing on changes in host oxidative stress parameters and antioxidant capacity (García-Ibarra et al, 2011). Overall, data showed that systemic infection by ACLSV did not produce any visible symptoms or membrane damage in leaves (i.e., no changes in lipid peroxidation), while antioxidant defenses increased, including GST.…”

Section: Gsts In Plant-virus Interactionsmentioning

confidence: 99%

Glutathione S-Transferase Enzymes in Plant-Pathogen Interactions

et al. 2018

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Plant glutathione S-transferases (GSTs) are ubiquitous and multifunctional enzymes encoded by large gene families. A characteristic feature of GST genes is their high inducibility by a wide range of stress conditions including biotic stress. Early studies on the role of GSTs in plant biotic stress showed that certain GST genes are specifically up-regulated by microbial infections. Later numerous transcriptome-wide investigations proved that distinct groups of GSTs are markedly induced in the early phase of bacterial, fungal and viral infections. Proteomic investigations also confirmed the accumulation of multiple GST proteins in infected plants. Furthermore, functional studies revealed that overexpression or silencing of specific GSTs can markedly modify disease symptoms and also pathogen multiplication rates. However, very limited information is available about the exact metabolic functions of disease-induced GST isoenzymes and about their endogenous substrates. The already recognized roles of GSTs are the detoxification of toxic substances by their conjugation with glutathione, the attenuation of oxidative stress and the participation in hormone transport. Some GSTs display glutathione peroxidase activity and these GSTs can detoxify toxic lipid hydroperoxides that accumulate during infections. GSTs can also possess ligandin functions and participate in the intracellular transport of auxins. Notably, the expression of multiple GSTs is massively activated by salicylic acid and some GST enzymes were demonstrated to be receptor proteins of salicylic acid. Furthermore, induction of GST genes or elevated GST activities have often been observed in plants treated with beneficial microbes (bacteria and fungi) that induce a systemic resistance response (ISR) to subsequent pathogen infections. Further research is needed to reveal the exact metabolic functions of GST isoenzymes in infected plants and to understand their contribution to disease resistance.

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Changes in the antioxidative metabolism induced by Apple chlorotic leaf spot virus infection in peach [Prunus persica (L.) Batsch]

Cited by 7 publications

References 37 publications

Evaluation of apricot (Prunus armeniaca L.) resistance to Apple chlorotic leaf spot virus in controlled greenhouse conditions

Evaluation of apricot (Prunus armeniaca L.) resistance to Apple chlorotic leaf spot virus in controlled greenhouse conditions

Implication of peroxidase activity in development of healthy and PPV-infected micropropagated GF305 peach plants

Glutathione S-Transferase Enzymes in Plant-Pathogen Interactions

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