Changes in the Concentration of Microvilli on the Free Surface of Healing Mesothelium Are Associated With Alterations in Surface Membrane Charge

Mutsaers, Steven E.; Whitaker, Darrel; Papadimitriou, John

doi:10.1002/(sici)1096-9896(199611)180:3<333::aid-path659>3.0.co;2-y

Cited by 34 publications

(13 citation statements)

References 25 publications

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“…However, it seems more likely that, by modulating microvilli density together with adhesion molecule expression, mesothelial cells can regulate trafficking of leucocytes into and out of the serosal cavities. This is supported by the observation that the greatest concentration of microvilli on mouse testicular mesothelial cells occurred at mesothelial cell junctions 6 days after injury, when regeneration of the mesothelium was almost complete and inflammatory cells were being cleared 34 …”

Section: Leucocyte Migrationmentioning

confidence: 69%

“…The entire microvilli population is also labile, with the number of microvilli expressed on each cell changing under different physiological conditions 15 . Differences in the number of microvilli on regenerating mesothelial cells have also been reported and it has been suggested that their concentration reflects functional adaptation 15,18,33,34 . These changes are also associated with an alteration in surface charge 34 .…”

Section: Microvillimentioning

confidence: 99%

“…He concluded from this observation that mesothelium could not heal solely by the centripetal migration of proliferating cells at the wound edge into the injured area, as occurs for healing of squamous epithelium. Since Hertzler's early observations, many studies using different types of serosal injury have been performed to elucidate the mechanisms regulating mesothelial regeneration, following deep lacerations, 67,244,245 broad abrasions, 46,246 minor linear scarifications, 247 drying, 248 chemical treatment 249 and heat injury 29–31,34,46,250 . Based on these studies, it is now generally accepted that the healing process begins, within 24 h of injury, upon the arrival at the wound surface of a population of round cells and is completed 7–10 days later when the area is covered by cells displaying all the characteristics of mesothelial cells 29–31,245,246 .…”

Section: Mesothelial Regenerationmentioning

confidence: 99%

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Mesothelial cells: Their structure, function and role in serosal repair

2002

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Mesothelial cells: Their structure, function and role in serosal repair MUTSAERS SE. Respirology 2002; 7: 171-191 The mesothelium is composed of an extensive monolayer of specialized cells (mesothelial cells) that line the body's serous cavities and internal organs. Traditionally, this layer was thought to be a simple tissue with the sole function of providing a slippery, non-adhesive and protective surface to facilitate intracoelomic movement. However, with the gradual accumulation of information about serosal tissues over the years, the mesothelium is now recognized as a dynamic cellular membrane with many important functions. These include transport and movement of fluid and particulate matter across the serosal cavities, leucocyte migration in response to inflammatory mediators, synthesis of pro-inflammatory cytokines, growth factors and extracellular matrix proteins to aid in serosal repair, release of factors to promote both the deposition and clearance of fibrin, and antigen presentation. Furthermore, the secretion of molecules, such as glycosaminoglycans and lubricants, not only protects tissues from abrasion, but also from infection and possibly tumour dissemination. Mesothelium is also unlike other epithelial-like surfaces because healing appears diffusely across the denuded surface, whereas in true epithelia, healing occurs solely at the wound edges as sheets of cells. Although controversial, recent studies have begun to shed light on the mechanisms involved in mesothelial regeneration. In the present review, the current understanding of the structure and function of the mesothelium and the biology of mesothelial cells is discussed, together with recent insights into the mechanisms regulating its repair.

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Section: Leucocyte Migrationmentioning

confidence: 69%

Section: Microvillimentioning

confidence: 99%

Section: Mesothelial Regenerationmentioning

confidence: 99%

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Mesothelial cells: Their structure, function and role in serosal repair

2002

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“…A murine model of mesothelial healing was used as previously described8. Briefly, mice were anaesthetized by an intraperitoneal injection of 0.05 mg/g body weight Nembutal (Bemac Laboratories, Australia).…”

Section: Methodsmentioning

confidence: 99%

Mesothelial regeneration is not dependent on subserosal cells

2000

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“…Uhrastructural and irnmunohistochemical characteristics have been described for HMCs and for mesothelial cells isolated from other animal species (rabbits, rats, mice, and dogs) (19,34,37,44). The PMCs obtained in our study exhibited uhrastructural features similar to those of in situ PMCs and of cultured HMCs, including microvilli, pinocytic vesicles, and junc- tional complexes at the cell-cell interface.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and functional characteristics of porcine peritoneal mesothelial cells

Ohan

Gilbert

Brouland³

et al. 1999

In Vitro Cell.Dev.Biol.-Animal

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The objective of this study was to establish a technique to isolate porcine mesothelial cells (PMCs) from omental tissue and to compare them to human mesothelial cells (HMCs). The PMCs were dispersed by collagenase digestion and isolated on a Ficoll layer. Their morphologic and ultrastructural features were assessed at confluence by light and electronic microscopy, and they were characterized by immunohistochemistry using specific HMC markers. PMC proliferation was studied in the presence of growth factors platelet-derived growth factor (PDGF), epidermal growth factor (EGF) or transforming growth factors beta1, beta2, or beta3 (TGF). Fibrinolytic PMC activity was detected by zymography for tissue plasminogen activator (tPA) and by reverse zymography for plasminogen activator inhibitor-1 (PAI-1). The recalcification time of cell lysates was used to define PMC procoagulant activity, and gelatinase zymography was used to detect metalloproteinase production. At confluence, PMCs formed typical cobblestone monolayers and exhibited structural features characteristic of HMCs. Weibel Palade bodies were never seen. Specific HMC markers (HBME1, ME1, WT1) cross-reacted with PMCs. As HMCs and PMCs coexpressed cytokeratin and vimentin, and also expressed vinculin and alpha-actin. Addition of PDGF or EGF to the culture medium stimulated PMC proliferation. PMCs constitutively expressed fibrinolytic and procoagulant activity and secreted MMP9 and MMP2. The technique described in this study allows isolation of mesothelial cells from porcine omental tissue. These porcine cells exhibit a mesothelial phenotype and functional properties similar to those of HMCs. Our data warrant an evaluation of mesothelial cells as targets in several therapeutic strategies with porcine models.

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Changes in the Concentration of Microvilli on the Free Surface of Healing Mesothelium Are Associated With Alterations in Surface Membrane Charge

Cited by 34 publications

References 25 publications

Mesothelial cells: Their structure, function and role in serosal repair

Mesothelial cells: Their structure, function and role in serosal repair

Mesothelial regeneration is not dependent on subserosal cells

Phenotypic and functional characteristics of porcine peritoneal mesothelial cells

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