Changes in the concentration of high-affinity oestradiol receptors in rat uterine supernatant preparations during the oestrous cycle, pseudopregnancy, pregnancy, maturation and after ovariectomy

Feherty, Patricia; Robertson, D. M.; Waynforth, H. B.; Kellie, A. E.

doi:10.1042/bj1200837

Cited by 96 publications

(28 citation statements)

References 15 publications

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“…0.9pmol/ uterus), in good agreement with previous reports (e.g. Gorski et al, 1970;Feherty et al, 1970 Under the cell-free reconstituted conditions the relative proportions of the 0.5M-KCl-extractable and residual receptors were dependent on the length of incubation. The nuclear uptake of [3H]oestradiol (presumably together with the receptor) in the presence of the uterine supernatant fraction was rapid into the soluble form, reaching a maximum value (approx.…”

Section: Distribution Of Oestrogen Receptors In the Uterus Of Controlsupporting

confidence: 81%

“…The concentration ofreceptor binding sites participating in the equilibrium was indicated at the intersection ofthe resulting straight line with the abscissa. Feherty et al (1970) showed that the technique yields identical results for both low-speed and high-speed cytosol uterine supernatant preparations.…”

Section: Cellfractionationmentioning

confidence: 88%

“…With the so-called physiological dose of the hormone (0.1lug/animal), the decrease of the receptor content 4h after injection is approx. 50% of the initial value (Sarff & Gorski, 1971), and this loss is only partly accounted for by the increase of the nuclear receptor concentration (Feherty, 1972;Milgrom et al, 1973). The cytosol receptor concentration is restored during the following period reaching approx.…”

mentioning

confidence: 99%

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Dynamics of oestrogen-receptor distribution between the cytosol and nuclear fractions of immature rat uterus after oestradiol administration

Mešter

1975

Biochemical Journal

135

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1. The nuclear-myofibrilar (800g pellet) fraction of the uterus from immature (22-23 days old) rats not exposed to oestrogen exhibits saturable binding of oestradiol. This nuclear binding capacity represents approximately 10% of that of the cytosol fraction (approx. 3.5fmol/ug of DNA). The predominant part (0.3 fmol/,ug of DNA) of the nuclear binding sites are present in the residual pellet after extraction with 0.5M-KCI. 2. By using an exchange technique in vitro, determinations of the nuclear binding sites have been carried out after administration of 1 ug of oestradiol in vivo. Within 0.5h after the hormone injection, the concentration of nuclear binding sites increased to approx. 0.4fmol/,ug of DNA in the 0.5M-KCl-extractable fraction, and to approx. 1.2fmol/cpg of DNA in the residual fraction. Meanwhile the cytosol oestrogen-receptor concentration decreased to approx. 10% of its initial value. In the following period from 0.5h after the oestradiol injection onwards, the concentration of nuclear oestrogen receptors decreased with halflife values of approx. 140 and 200min for the KCI (0.5M)-extractable and residual form respectively. At the same time, the cytosol receptor concentration increased to reach approx. 50% of the initial value by 6h. This increase could not be blocked by cycloheximide. The initial concentration of cytosol receptor was restored approx. 11 h after the injection and the increase during the 6-l1 h period was sensitive to cycloheximide inhibition, suggesting protein-synthesis-dependence of the process. 3. With the (more) physiological dose of oestradiol (O.1,ug), the decrease of the cytosol receptor was only 50% by 4h and this was followed by a period (up to 12h after injection) during which the initial concentration was restored. During this period the increase of the receptor can be blocked by cycloheximide.Injection of oestradiol into immature rats rapidly leads to a decrease of the concentration of uterine cytosol high-affinity oestrogen-binding sites (receptor) and to an increase of the nuclear oestrogen receptor concentration (e.g. per total uterus). With the so-called physiological dose of the hormone (0.1lug/animal), the decrease of the receptor content 4h after injection is approx.

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Section: Distribution Of Oestrogen Receptors In the Uterus Of Controlsupporting

confidence: 81%

Section: Cellfractionationmentioning

confidence: 88%

mentioning

confidence: 99%

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Dynamics of oestrogen-receptor distribution between the cytosol and nuclear fractions of immature rat uterus after oestradiol administration

Mešter

1975

Biochemical Journal

135

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“…In the rat, E z receptor concentration declines soon after mating and remains at a relatively low level until parturition (Feherty et al 1970). Davies and Ryan (1973)found a progressive rise in the myometrial P receptor level until about day 9 of pregnancy in the rat followed by a progressive fall until parturition.…”

Section: Discussionmentioning

confidence: 99%

Uterine and Placental Growth: RNA and Protein Metabolism and Steroid Hormone Receptor Levels in the Endometrium, Whole Uterus and Cotyledons during Pregnancy in the Ewe

Miller¹,

Stone²

1981

Aust. Jnl. Of Bio. Sci.

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Some aspects of uterine and placental growth have been examined during pregnancy in the ewe. Changes in in vitro rates of protein synthesis, RNA:DNA and protein:DNA ratios and the tissue concentration of DNA in intercaruncular endometrium and caruncles/cotyledons between days 0 (oestrus) and 112 of pregnancy were compared with corresponding changes in the concentrations of high-affinity cytosol receptors for oestradiol and progesterone in whole uterus and caruncles/maternal cotyledons. Rapid growth of the intercaruncular endometrium between days 28 and 112 and of the developing cotyledons between days 28 and 84 occur in the presence of tissue levels of both steroid receptors that are extremely low in relation to the corresponding levels seen in the uterus at oestrus. If uterine responses to steroid hormones are regulated by the amounts of specific receptor present in the tissue, the results support the concept that uterine growth after day 28 of pregnancy results primarily from the physical stimulus of the growing conceptus rather than from the actions of endogenous steroid sex hormones.

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“…1). However, it could be due to differences in the procedures used to assay receptors (Duffy & Duffy, 1977b;Feherty et al, 1970). …”

mentioning

confidence: 99%

Peroxidase as a marker for oestrogen dependence in human breast cancer

Collings¹,

Savage²

1979

Br J Cancer

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Changes in the concentration of high-affinity oestradiol receptors in rat uterine supernatant preparations during the oestrous cycle, pseudopregnancy, pregnancy, maturation and after ovariectomy

Cited by 96 publications

References 15 publications

Dynamics of oestrogen-receptor distribution between the cytosol and nuclear fractions of immature rat uterus after oestradiol administration

Dynamics of oestrogen-receptor distribution between the cytosol and nuclear fractions of immature rat uterus after oestradiol administration

Uterine and Placental Growth: RNA and Protein Metabolism and Steroid Hormone Receptor Levels in the Endometrium, Whole Uterus and Cotyledons during Pregnancy in the Ewe

Peroxidase as a marker for oestrogen dependence in human breast cancer

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