Changes in the concentration of tissue inhibitor of type 1 metalloproteinases in blood serum and liver of mice with CCl4-induced hepatitis

Abstract

The concentration of tissue inhibitor of type 1 metalloproteinases in blood serum from intact CBA mice measured by enzyme immunoassay is similar to that in healthy humans. The concentration of tissue inhibitor of type 1 metalloproteinases in mouse bile was higher than in blood serum, while its concentration in liver homogenate more than 1000-fold exceeded that in the serum, which attests to its primarily intracellular localization in the liver. Loading of liver cell lysosomes with Triton WR-1339 and development of intrahepatic cholestasis did not affect the concentration of tissue inhibitor of type 1 metalloproteinases in liver homogenate and bile. Administration of CCl4 to mice for 4.5 weeks was accompanied by an increase in the concentration of tissue inhibitor of type 1 metalloproteinases in blood serum, but not in liver homogenate. These changes reflect dysregulation of metalloproteinases, development of inflammation, and progression of the initial stage of connective tissue formation in mouse liver.

Effect of Atorvastatin on Activities of Matrix Metalloproteinases and Chitotriosidase in Male and Female Mice with Experimental Hyperlipidemia

“…Functional activity of this enzyme is believed to be associated with innate immunity. Decreased serum CT activity in mice with moderate hyperlipidemia refl ects abnormality of liver functional tests during Tritoninduced lipidosis [1].…”

“…Matrix metalloproteinases (MMP) are involved in various stages of vascular wall remodeling and capsular formation or rupture in the atherosclerotic plaque. It is accompanied by migration and transformation of macrophages into foam cells [1,11,15]. The role of various types of MMP and macrophage activation in the development of hyperlipidemia at various stages of atherosclerosis remains unclear.…”

Fractional Composition of Blood Serum Lipoproteins in Mice and Rats with Triton WR 1339-Induced Lipemia

“…The fractional composition of blood serum LP was estimated by means of small-angle X-ray scattering [1][2][3]. This method allowed us to perform quantitative analysis for the total concentration of LP cholesterol (CH), LP TG, and fractions or subfractions of LP (HDL, LDL, and VLDL; except for chylomicrons) [1,2].…”

“…The model of lipemia can be used to study the role of fractional composition of lipoproteins and, particularly, of triglycerides in the pathogenesis of atherosclerosis. Moreover, this model holds much promise for evaluation of the effi ciency of hypolipidemic drugs (statins and fi brates) in normalizing the increased level of atherogenic cholesterol of VLDL and LDL.Lipemia is one of the major risk factors for atherosclerosis, cardiovascular diseases, and cerebrovascular disorders [1][2][3][4]. The model of lipemia in experimental animals induced by Triton WR 1339 (Triton) is used in studies of atherosclerosis pathogenesis and effect of hypolipidemic drugs (statins and fi brates) [5,11].…”

“…Lipemia is one of the major risk factors for atherosclerosis, cardiovascular diseases, and cerebrovascular disorders [1][2][3][4]. The model of lipemia in experimental animals induced by Triton WR 1339 (Triton) is used in studies of atherosclerosis pathogenesis and effect of hypolipidemic drugs (statins and fi brates) [5,11].…”