Changes in the expression of genes related to the biosynthesis of catechins in tea (<i>Camellia sinensis</i>L.) under greenhouse conditions

Liu, M.; Chen, X. J.; Qi, Xiaohua; Xu, Qiang; Chen, X. H.

doi:10.1080/14620316.2015.11513166

Cited by 6 publications

(3 citation statements)

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“…The synthesis of EGCG was promoted at 30 °C, which was consistent with our previous study ( Xiang et al., 2021a ). From the perspective of temperature, it is confirmed that the EGCG content of tea planted in greenhouse is higher than that of field planting, and the EGCG content of tea in autumn is higher than that in spring ( Liu et al., 2015a ; Liu et al., 2015b ). CHS and ANS , as key genes in EGCG synthesis pathway, directly affect the biosynthesis of EGCG ( Yin et al., 2020 ).…”

Section: Discussionmentioning

confidence: 90%

“…Previous studies showed that the contents of epigallocatechin (EGC), epicatechin (EC), epicatechin gallate (ECG) and epigallocatechin gallate (EGCG) in tea were increased along with the elevation of daily temperature ( Wang et al., 2011 ). The expression of flavanone 4-reductase ( DFR ) and anthocyanidin reductase ( ANR ) genes were up-regulated in tea plants that cultivated in the greenhouse, which promoted the accumulation of catechins and EGCG ( Liu et al., 2015a ). The EGCG content is positively relative to the temperature under artificial temperature control ( Xiang et al., 2021a ).…”

Section: Introductionmentioning

confidence: 99%

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The biosynthesis of EGCG, theanine and caffeine in response to temperature is mediated by hormone signal transduction factors in tea plant (Camellia sinensis L.)

Zhu

Liu

et al. 2023

Front. Plant Sci.

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As the main flavor components of tea, the contents of epigallocatechin-3-gallate (EGCG), theanine and caffeine are regulated by ambient temperature. However, whether the biosynthesis of EGCG, theanine and caffeine in response to temperature is regulated by endogenous hormones and its mechanism is still unclear. In this study, tea cuttings cultivated in the phytotron which treated at different temperatures 15℃, 20℃, 25℃ and 30℃, respectively. The UPLC and ESI-HPLC-MS/MS were used to determine the contents of EGCG, theanine, caffeine and the contents of phytohormones in one leaf and a bud. The results showed that indoleacetic acid (IAA), gibberellin 1(GA1) and gibberellin 3 (GA3) were significantly correlated with the content of EGCG; Jasmonic acid (JA), jasmonate-isoleucine (JA-Ile) and methyl jasmonate (MeJA) were strongly correlated with theanine content; IAA, GA1 and gibberellin 4 (GA4) were significantly correlated with caffeine content at different temperatures. In order to explore the internal intricate relationships between the biosynthesis of these three main taste components, endogenous hormones, and structural genes in tea plants, we used multi-omics and multidimensional correlation analysis to speculate the regulatory mechanisms: IAA, GA1 and GA3 up-regulated the expressions of chalcone synthase (CsCHS) and trans-cinnamate 4-monooxygenase (CsC4H) mediated by the signal transduction factors auxin-responsive protein IAA (CsIAA) and DELLA protein (CsDELLA), respectively, which promoted the biosynthesis of EGCG; IAA, GA3 and GA1 up-regulated the expression of CsCHS and anthocyanidin synthase (CsANS) mediated by CsIAA and CsDELLA, respectively, via the transcription factor WRKY DNA-binding protein (CsWRKY), and promoted the biosynthesis of EGCG; JA, JA-Ile and MeJA jointly up-regulated the expression of carbonic anhydrase (CsCA) and down-regulated the expression of glutamate decarboxylase (CsgadB) mediated by the signal transduction factors jasmonate ZIM domain-containing protein (CsJAZ), and promoted the biosynthesis of theanine; JA, JA-Ile and MeJA also jointly inhibited the expression of CsgadB mediated by CsJAZ via the transcription factor CsWRKY and AP2 family protein (CsAP2), which promoted the biosynthesis of theanine; IAA inhibited the expression of adenylosuccinate synthase (CspurA) mediated by CsIAA via the transcription factor CsWRKY; GA1 and gibberellin 4 (GA4) inhibited the expression of CspurA mediated by CsDELLA through the transcription factor CsWRKY, which promoted the biosynthesis of caffeine. In conclusion, we revealed the underlying mechanism of the biosynthesis of the main taste components in tea plant in response to temperature was mediated by hormone signal transduction factors, which provided novel insights into improving the quality of tea.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The biosynthesis of EGCG, theanine and caffeine in response to temperature is mediated by hormone signal transduction factors in tea plant (Camellia sinensis L.)

Zhu

Liu

et al. 2023

Front. Plant Sci.

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“…For purple tea, CsDFRa in 2~3 leaves (purple) was 2-folder higher than that in 4~5 leaves (green) of Zijuan variety [42]. CsDFR is usually downregulated under stresses, such as drought [18] and low temperature [43]. Other putative CsDFRs showed the same trends, but their expression levels were very low [13–15].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of putative dihydroflavonol 4-reductase gene family in tea plant

et al. 2019

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One identified dihydroflavonol 4-reductases (DFR) encoding gene (named as CsDFRa herein) and five putative DFRs (named as CsDFRb1, CsDFRb2, CsDFRb3, CsDFRc and CsDFRd) in tea (Camellia sinensis) have been widely discussed in recent papers concerning multi-omics data. However, except for CsDFRa, their function and biochemical characteristics are not clear. This study aims to compare all putative CsDFRs and preliminarily evaluate their function. We investigated the sequences of genes (coding and promoter regions) and predicted structures of proteins encoded, and determined the activities of heterologously expressed CsDFRs under various conditions. The results showed that the sequences of five putative CsDFRs were quite different from CsDFRa, and had lower expression levels as well. The five putative CsDFRs could not catalyze three dihydroflavonol substrates. The functional CsDFRa had the strongest affinity with dihydroquercetin, and performed best at pH around 7 and 35°C but was not stable at lower pHs or higher temperatures. Single amino acid mutation at position 141 modified the preference of CsDFRa for dihydroquercetin and dihydromyricetin, and also weakened its stability. These data suggest that only CsDFRa works in the pathway for generating anthocyanidins and catechins. This study provides new insights into the function of CsDFRs and may assist to develop new strategies to manipulate the composition of tea flavonoids in the future.

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De novo leaf transcriptome of a triploid linalool chemotype of Lippia alba (Mill.) N.E.Br. ex Britton & P. Wilson

et al. 2021

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Changes in the expression of genes related to the biosynthesis of catechins in tea (Camellia sinensisL.) under greenhouse conditions

Cited by 6 publications

References 1 publication

The biosynthesis of EGCG, theanine and caffeine in response to temperature is mediated by hormone signal transduction factors in tea plant (Camellia sinensis L.)

The biosynthesis of EGCG, theanine and caffeine in response to temperature is mediated by hormone signal transduction factors in tea plant (Camellia sinensis L.)

Comprehensive analysis of putative dihydroflavonol 4-reductase gene family in tea plant

De novo leaf transcriptome of a triploid linalool chemotype of Lippia alba (Mill.) N.E.Br. ex Britton & P. Wilson

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