Changes in the Gene Expression of the White Rot Fungus<i>Phanerochaete chrysosporium</i>Due to the Addition of Atropine

Minami, Masahiko; Suzuki, Kaoru; Shimizu, Akifumi; Hongo, T.; Sakamoto, Takako; Ohyama, Naoki; Kitaura, Hironori; Kusaka, Akiho; Iwama, Kenji; Irie, Toshikazu

doi:10.1271/bbb.80870

Cited by 11 publications

(13 citation statements)

References 26 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, these results were inconclusive because Mn 2+ -dependent oxidation activity, which represents the total activity of MnP and VP, was the same in both strains (Yao et al 2013). In P. chrysosporium, our previous studies suggested that the CaM gene was induced by an increase of intracellular cAMP concentration (Minami et al 2007(Minami et al , 2009Sakamoto et al 2010Sakamoto et al , 2012 and that CaM positively regulates the expression of almost all genes encoding isozymes of LiP and MnP (Sakamoto et al 2010(Sakamoto et al , 2012. Because the present study suggested that cAMP also induces transcription of the CaM gene in P. ostreatus, similar to P. chrysosporium (Fig.…”

Section: Discussionsupporting

confidence: 70%

“…In P. chrysosporium, the CaM gene was suggested to be induced by intracellular 3′-5′-cyclic adenosine monophosphate (cAMP) at the transcription level (Minami et al 2007(Minami et al , 2009Sakamoto et al 2012). To investigate whether the CaM gene is also transcriptionally regulated by intracellular cAMP in P. ostreatus, we measured the absolute quantity of gene transcript after 7 days of culture of a cAMP-overproducing strain, which was previously made by introducing a mutated Gα isoform gene (Yao et al 2013).…”

Section: Cam Gene Transcription In a Camp-overproducing Strainmentioning

confidence: 99%

“…In fungi, CaM is required for hyphal growth, morphogenesis, germination, and penetration into plant tissue (Ahn and Suh 2007;Ahn et al 2003;Sato et al 2004;Wang et al 2006). In our previous study, we found that CaM gene expression is regulated in parallel with genes encoding most isozymes of LiP and MnP in P. chrysosporium (Minami et al 2007(Minami et al , 2009. Furthermore, the addition of the CaM inhibitor W7 reduces the amount of LiP and MnP isozyme transcripts, leading to reduced activity of these enzymes (Sakamoto et al 2010(Sakamoto et al , 2012.…”

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus

et al. 2014

Self Cite

View full text Add to dashboard Cite

Previously, we suppressed the expression of genes encoding isozymes of lignin peroxidase (LiP) and manganese peroxidase (MnP) using a calmodulin (CaM) inhibitor, W7, in the white-rot fungus Phanerochaete chrysosporium; this suggested that CaM positively regulates their expression. Here, we studied the role of CaM in another white-rot fungus, Pleurotus ostreatus, which produces MnP and versatile peroxidase (VP), but not LiP. W7 upregulated Mn(2+)-dependent oxidation of guaiacol, suggesting that CaM negatively regulates the production of the enzymes. Suppression of CaM in P. ostreatus using RNAi also led to upregulation of enzyme activity, whereas overexpression of CaM in P. ostreatus caused downregulation. Real-time RT-PCR showed that MnP1-6 and VP3 levels in the CaM-knockdown strain were higher than those in the wild-type strain, while MnP-5 and -6 and VP1 and 2 levels in the CaM-overexpressing strain were lower than in the wild type. Moreover, we also found that another ligninolytic enzyme, laccase, which is not produced by P. chrysosporium, was negatively regulated by CaM in P. ostreatus similar to MnP and VP. Although overexpression of CaM did not reduce the ability of P. ostreatus to digest beech wood powder, the percentage of lignin remaining in the digest was slightly higher than in the wild-type strain digest.

show abstract

Section: Discussionsupporting

confidence: 70%

Section: Cam Gene Transcription In a Camp-overproducing Strainmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…In nitrogen-limited, oxygen purged cultures, upregulated transcripts correspond to proteins related to nitrogen starvation, including oligopeptide transporters (Vanden Wymelenberg et al 2009;Wu and Zhang 2010), proteins related to stress response (Wu and Zhang 2010;Minami et al 2009), signal transduction (Wu and Zhang 2010;Minami et al 2009Minami et al , 2007, RNA processing (Wu and Zhang 2010), posttranslational modification (Vanden Wymelenberg et al 2009;Wu and Zhang 2010), and protein synthesis (Wu and Zhang 2010;Minami et al 2007) as well as proteases (Vanden Wymelenberg et al 2009;Minami et al 2007) and cytochrome P450s (Vanden Wymelenberg et al 2009;Minami et al 2009). Membrane-associated proteins that are upregulated in ligninolytic conditions have been identified by proteomic analysis of isolated microsomal membrane fractions from P. chrysosporium RP-78 and RT-qPCR of corresponding transcripts.…”

Section: Differential Gene Expression In Phanerochaete Species Grown mentioning

confidence: 99%

“…Notably, addition of atropine does not increase hyphal weight; and the atropine analog, pilocarpine, does not inhibit either LiP or MnP expression (Minami et al 2009). Accordingly, to help deconstruct the cAMP pathway leading to the production of ligninolytic enzymes, cDNA libraries were created from P. chrysosporium RP-78 exposed to atropine (Minami et al 2009). Cultures were grown under low nitrogen conditions with atropine added after two days of incubation.…”

Section: Differential Gene Expression In Phanerochaete Species Grown mentioning

confidence: 99%

Expression and regulation of genes encoding lignocellulose-degrading activity in the genus Phanerochaete

MacDonald

Suzuki

Master

2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

As white-rot basidiomycetes, Phanerochaete species are critical to the cycling of carbon sequestered as woody biomass, and are predicted to encode many enzymes that can be harnessed to promote the conversion of lignocellulose to sugars for fermentation to fuels and chemicals. Advances in genomic, transcriptomic, and proteomic technologies have enabled detailed analyses of different Phanerochaete species and have revealed numerous enzyme families required for lignocellulose utilization, as well as insight into the regulation of corresponding genes. Recent studies of Phanerochaete are also exemplified by molecular analyses following cultivation on different wood preparations, and show substrate-dependent responses that were difficult to predict using model compounds or isolated plant polysaccharides. The aim of this mini-review is to synthesize results from studies that have applied recent advances in molecular tools to evaluate the expression and regulation of proteins that contribute to lignocellulose conversion in Phanerochaete species. The identification of proteins with as yet unknown function are also highlighted and noted as important targets for future investigation of white-rot decay.

show abstract

Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium

et al. 2010

View full text Add to dashboard Cite

We investigated the effects of a calmodulin (CaM) inhibitor, W-7, on the expression of lignin peroxidase (LiP) and manganese peroxidase (MnP) genes in Phanerochaete chrysosporium to consider the role of cam gene, which was upregulated in parallel with the total activities of LiP and MnP in our previous transcriptomic analysis. The addition of 100 μM W-7 to the fungal cultures repressed the total activities of LiP and MnP, whereas the addition of 100 μM W-5, which is a control drug of W-7, retained approximately half of them, indicating that the effect of W-7 was attributable to CaM inhibition. Real-time reverse transcription polymerase chain reaction analysis revealed that most of lip and mnp isozyme genes predicted from whole-genome data were significantly inhibited by W-7 at the transcription level (P ≤ 0.05). These results suggest that CaM has an important role for the expression of isozyme genes of LiP and MnP at the transcription level.

show abstract

Changes in the Gene Expression of the White Rot FungusPhanerochaete chrysosporiumDue to the Addition of Atropine

Cited by 11 publications

References 26 publications

Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus

Effects of calmodulin on expression of lignin-modifying enzymes in Pleurotus ostreatus

Expression and regulation of genes encoding lignocellulose-degrading activity in the genus Phanerochaete

Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium

Contact Info

Product

Resources

About