Changes in the Homeostatic Mechanism of Dental Pulp with Age: Expression of the Core-binding Factor Alpha-1, Dentin Sialoprotein, Vascular Endothelial Growth Factor, and Heat Shock Protein 27 Messenger RNAs

Abstract

Aim: Dental pulp has various characteristics in the pulp chamber, but only a few biological evaluations about the effect of age on dental pulp tissue have been reported. The purpose of this study was to compare dental pulp from young and from adult rats to characterize the homeostatic mechanism.Methodology: Dental pulp cells (DPCs) were obtained from the first molar of rats, weighing 150 g each for the young group and 350 g each for the adult group. Expression of Cbfa-1, VEGF or HSP27 mRNAs by cultured pulp cells was determined using a quantitative real time PCR system after 3, 7 or 14 days.Results: The expression of Cbfa-1 mRNA in the young group was higher than in the adult group. Expression of VEGF and HSP27 mRNAs in the adult group was higher than in the young group. ConclusionThe self defense system in young DPCs is undertaken by calcification, but in adult DPCs it is carried out by the expression of self defense proteins and the regeneration of vessels.

Heat Stress Activates Interleukin-8 and the Antioxidant System via Nrf2 Pathways in Human Dental Pulp Cells

“…Consistent with the results showing enhanced Nrf2 translocation in response to heat exposure, the expression levels of SOD, HO-1, GST, GR, and GCL were increased. These results are consistent with the results of another study showing that the self-defense system in adult dental pulp cells involves the expression of self-defense proteins (39).…”

“…NAC is a cysteine derivative that is more stable than glutathione and protects cells against oxidative stress in various ways (39). In our study, the addition of NAC to cells blocked heat-induced IL-8 and Nrf2 target gene expression (Fig.…”

Molecular and cellular mechanisms of aortic stenosis

“…As mentioned above already, VEGF has been shown to play an important role in bone formation besides its angiogenic activity. Indeed, it has been shown that VEGF is upregulated at the sites of bone formation [72] and is involved in bone healing [73]. These data indicate that VEGF -as an inflammatory mediator -plays a functional role in bone formation and thereby calcification.…”

Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation

“…Further, Tate et al [16] and Suzuki et al [17] verified the expression of HSP in dental pulp cells after experimental cavity preparation by laser ablation. Moreover, Matsuzaki et al [18] reported an increased in mRNA levels of HSP27 in the pulp. However, the expression of HSP27 in dental pulp cells after teeth separation has not been investigated.…”