Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation

Abstract

AimAfter immediate teeth separation, expression of HSP27 in the mouse dental pulp was examined. Immunohistochemistry was performed to examine the incidence of HSP27 expression.Materials and methodsA total of 36 8-week-old ddY mice were used as experimental subjects and a wedge was inserted in between maxillary right molars. The wedge was removed 30 min or 3 h after insertion. Animals were immediately sacrificed after the removal of wedge or until 1 week later and serial sections from paraffin-embedded tissues were prepared. Immunohistochemistry was carried out to examine the expression of HSP27. The untreated side served as the control.ResultsIn the control group, the endothelial cells and some pulp fibroblasts weakly expressed HSP27 suggesting that the expression is due to mechanical stress brought about by physiological masticatory force and pressure from the tongue. In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter. The expression decreased at the same level as that of the control group 1 week after wedge removal.ConclusionHSP27 may serve as an indicator of stimulus strong enough to show its expression.

Placental proteome alterations in women with intrahepatic cholestasis of pregnancy

“…Heat shock proteins are induced by heat and a variety of other physical, chemical, and biologic stressors such as ischemia, infection, and inflammation [18,19]. They have roles in cell repair and defense mechanisms [20]. Some heath shock proteins such as HSPA5 are also upregulated in many types of cancers.…”

Regeneration of Dentin Using Stem Cells Present in the Pulp

“…Additionally, ALP expressed in the early stage of calcification associated with odontoblast differentiation is an index of odonoblastic activity [33]. In our previous studies, we induced the differentiation of dental pulp cells by adding injurious stimulation to the periodontium and pulp [10,11]. Nabeyama et al have shown that there is a weak positive reaction for Runx2 in the pulp tissue that has not been artificially stimulated [10].…”

“…Saito et al stimulated the pulp by the same experimental method as Nabeyama et al and examined the expression of HSP27 [11]. The pulp showed a weak positive reaction for HSP27 from the immunohistochemical reaction of the control group.…”

“…We have shown previously that orthodontic mechanical stress and artificial inflammation stimulate the cells of the periodontium and dental pulp to express various cell differentiation factors. The osteoblast marker alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), which induces osteoblast differentiation, and heat shock proteins (HSPs), which are involved in homeostasis and cell differentiation, may be strongly expressed in cells exposed to injurious stimuli [7][8][9][10][11][12]. Moreover, periodontal tissue and dental pulp respond to mechanical stresses and inflammation, causing periodontal tissue remodeling and expression of cell differentiation-related factors [13].…”

“…Moreover, periodontal tissue and dental pulp respond to mechanical stresses and inflammation, causing periodontal tissue remodeling and expression of cell differentiation-related factors [13]. Furthermore, orthodontic mechanical stress on the periodontium causes activation of hard tissue-forming cells in the pulp tissue [10,11]. This indicates the potential of dental pulp stem cells to differentiate into hard tissue-forming cells upon stimulation.…”