Changes in the kinetics of plasma chemiluminescence as a measure of systemic oxidative stress in humans

Sozarukova, Madina M.; Polimova, A. M.; Проскурнина, Е. В.; Vladimirov, Yu. A.

doi:10.1134/s0006350916020202

“…As in blood, uric acid is the main water‐soluble antioxidant in saliva (Moore et al., 1994). The difference lies in albumin contributing to the antioxidant profile for blood (Sozarukova et al., 2016). Thus, based on changes in salivary antioxidant potential, we can suppose that studies of the systemic influence of psychological comfort on ROS homeostasis would be promising.…”

Section: Discussionmentioning

confidence: 99%

Touch‐induced emotional comfort results in an increase in the salivary antioxidant potential: A correlational study

Проскурнина

¹

,

Sokolova

²

,

Portnova

³

2021

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Psychoemotional discomfort results in oxidative stress, as proven by many studies. One of the hypotheses states that this effect is realized through the sympathetic system. For example, acute and chronic emotional distress led to a decrease in the concentration of magnesium, an increase in malondialdehyde, and the depletion of the antioxidant potential in young people stressed by a military situation (Cernak et al., 2000). Increased concentrations of plasma myeloperoxidase, interleukin-6, and salivary cortisol in musicians on the following day of the premiere concert indicate that stress of public performance leads to oxidative stress (Pilger et al., 2014). Acute myocardial infarction in stressed rats (the stress was caused by daylight/darkness exposure, overcrowding, isolation, new hierarchy, tilting the cage, and restriction of water or food) was accompanied by hyperactivation of the sympathetic system, increased malondialdehyde and peroxynitrite, and decreased superoxide dismutase and glutathione (Mercanoglu et al., 2008). Whisker removal-induced

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“…The CL parameters (Imax, S) also exceeded the control group's values and depended on disease severity. The intensity of induced CL (Imax) in the blood serum reflects the potential ability of lipid substrates to form highly active electron-excited products [ 22 , 23 ]. Accordingly, the registered increased Imax values confirm the activation of free radical reactions in all examined COPD patients.…”

Section: Discussionmentioning

confidence: 99%

The Differences in the Levels of Oxidative Status Marker and Soluble CD95 in Patients with Moderate to Severe COPD during an Exacerbation and a Stable Period

Soodaeva

¹

,

Kubysheva

²

,

Klimanov

³

et al. 2021

Oxidative Medicine and Cellular Longevity

2

0

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Studying the features of changes in markers of oxidative stress (OS) and inflammation indicators in COPD patients depending on the degree of bronchial obstruction is one of the priority directions for improving the prognosis and monitoring of the course of this pathology. We conducted a comparative investigation of changes in markers of OS and apoptosis at the systemic and local levels in patients with moderate to severe COPD during exacerbation and stable phase. 105 patients with COPD aged 46-67 and 21 healthy nonsmoking volunteers comparable in age were examined. COPD patients were divided into four groups: moderate COPD (GOLDII) during the exacerbation (GOLDIIex, n = 25 ) and in the stable phase (GOLDIIst, n = 27 ), severe COPD (GOLDIII) during the exacerbation (GOLDIIIex, n = 29 ), and in the stable phase (GOLDIIIst, n = 24 ). We studied the levels of such lipid peroxidation (LPO) products as diene conjugates (DC) and Schiff bases (SB) and parameters of induced chemiluminescence (Imax, total light sum-S, Imax/S) in blood serum, as well as sCD95 concentration in blood and exhaled breath condensate (EBC). The relationship between the values of the OS system indicators with sCD95, as well as with the parameters of lung function, was investigated. Multidirectional changes in OS indicator levels in COPD patients depending on the severity of obstructive airway disorders have been established. The maximum values of DC ( 0.26 ± 0.046 RU ), Imax ( 0.265 ± 0.19 RLU), and Imax/S ( 0.13 ± 0.05 ) were typical for patients with moderate COPD, while the highest SB level ( 5.7 ± 2.3 RU) was observed in severe COPD during an exacerbation. The exacerbation of the disease was characterized by an increase in DC concentration in both GOLDIIex ( 0.26 ± 0.046 RU) and GOLDIIIex ( 0.209 ± 0.02 RU) compared to the stable moderate and severe COPD ( 0.202 ± 0.028 RU and 0.19 ± 0.03 RU, respectively, p < 0.05 ). The established decrease in high values of DC, Imax, Imax/S, and sCD95 and an increase in SB concentration in GOLD III can serve as quantitative indicators of the prognosis of the severity of the disease. The serum concentration of sCD95 in GOLDIIex ( 366.4 ± 70.5 U/ml) and GOLDIIst ( 361.4 ± 72.8 U/ml) did not differ from the control group ( 393.7 ± 80.9 U/ml, p > 0.05 ). In patients with FEV 1 < 49 % during the exacerbation and stable phase, the serum levels of Imax/S ( 0.058 ± 0.01 and 0.062 ± 0.01 ) and sCD95 ( 318.2 ± 66.3 U/ml and 321.4 ± 42.5 U/ml) were lower than the values of healthy volunteers ( 0.08 ± 0.01 and 393.7 ± 80.9 U/ml, respectively, p < 0.05 ). A positive correlation between sCD95 concentration and airway obstruction degree in all examined COPD patients was established. The revealed numerous associations between sCD95 and OS marker levels in GOLDIII indicate a relationship between systemic radical stress and apoptosis processes both in the respiratory tract and the whole body under conditions of severe inflammation. The established correlations between the values of DC, Imax, and sCD95 in the blood serum and the lung function parameters in all studied patients allow us to consider these indicators as additional prognostic indicators of disease intensification. Our work results help clarify the participation and detail of FRO and apoptosis processes in developing pathophysiological features in moderate to severe COPD in different periods and, accordingly, improve the efficiency of diagnosis and treatment of the disease.

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“…According to the results obtained, we can assume that the tangent of the chemiluminescence rise angle (tg α) characterizes the oxidation of albumin: the more oxidized the albumin, the lower the value of tg α. The chemiluminograms were characterized by complex kinetics, each component of which can be described by quantitative indices (Figure 3b) [38], where ICL-a new level of steady-state luminescence after the addition of albumin, tg α-the tangent of the chemiluminescence rise angle and SCL-area of chemiluminescence suppression. According to our non-published data, the difference ICL − I0 reflects the level of the non-oxidized group -SH, i.e., the concentration of mercaptoalbumin.…”

Section: Antioxidant Capacity Of Albuminmentioning

confidence: 99%

Effect of Low-Dose Line-Spectrum and Full-Spectrum UV on Major Humoral Components of Human Blood

Sozarukova,

Skachko,

Chilikina

et al. 2023

Molecules

0

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Ultraviolet blood irradiation (UVBI) is an alternative approach to the treatment of infectious diseases of various pathogeneses. Recently, UVBI has attracted particular interest as a new immunomodulatory method. Experimental studies available in the literature demonstrate the absence of precise mechanisms of the effect of ultraviolet radiation (UV) on blood. Here, we investigated the effect of UV radiation of line-spectrum mercury lamp (doses up to 500 mJ/cm2) traditionally used in UVBI on the major humoral blood components: albumin, globulins and uric acid. Preliminary data on the effect of various doses of UV radiation of full-spectrum flash xenon lamp (doses up to 136 mJ/cm2), a new promising source for UVBI, on the major blood plasma protein, albumin, are presented. The research methodology included spectrofluorimetric analysis of the oxidative modification of proteins and analysis of the antioxidant activity of humoral blood components by chemiluminometry. The effect of UV radiation on albumin caused its oxidative modification and, accordingly, an impairment of the transport properties of the protein. At the same time, UV-modified albumin and γ-globulins acquired pronounced antioxidant properties compared to native samples. Uric acid mixed with albumin did not protect the protein against UV-induced oxidation. The flash full-spectrum UV qualitatively had the same effect on albumin as line-spectrum UV did, but an order of magnitude lower doses were required to achieve comparable effects. The suggested protocol can be used for selecting a safe individual dose for UV therapy.

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Changes in the kinetics of plasma chemiluminescence as a measure of systemic oxidative stress in humans

Cited by 21 publications

References 18 publications

Touch‐induced emotional comfort results in an increase in the salivary antioxidant potential: A correlational study

Touch‐induced emotional comfort results in an increase in the salivary antioxidant potential: A correlational study

The Differences in the Levels of Oxidative Status Marker and Soluble CD95 in Patients with Moderate to Severe COPD during an Exacerbation and a Stable Period

Effect of Low-Dose Line-Spectrum and Full-Spectrum UV on Major Humoral Components of Human Blood

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