Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol

Martiniaková, Monika; Sarocka, Anna; Babošová, Ramona; Großkopf, Birgit; Kapusta, Edyta; Goc, Zofia; Formicki, Grzegorz; Omelka, Radoslav

doi:10.1186/s40709-018-0079-1

Cited by 10 publications

(11 citation statements)

References 47 publications

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“…In fact, trabecular thickness, trabecular number, and percent bone density was affected by ethanol-exposed animals. A recent study reported that trabecular structures (i.e., thickness and number) were reduced in sub-chronic ethanol consumption protocol [ 38 ], which supports our findings. Likewise, it is well established that ethanol consumption displays oxidative unbalance and pro-inflammatory processes [ 39 ].…”

Section: Discussionsupporting

confidence: 92%

Ethanol binge drinking exposure affects alveolar bone quality and aggravates bone loss in experimentally-induced periodontitis

et al. 2020

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Background Periodontitis is a multifactorial inflammatory disease of tooth supporting tissues caused by oral biofilms, influenced by environmental and genetic factors, among others. Ethanol consumption has been considered a factor that enhances alveolar bone loss, especially in high doses. The present study aims to investigate the changes promoted by ethanol binge drinking per se or associated with ligature-induced periodontal breakdown on alveolar bone loss. Materials and methods Thirty-two Wistar rats were randomly allocated into four groups: control (C), ethanol (3g/kg/day; 3 days On-4 days Off protocol by gavage for 28 days, EtOH), experimental periodontitis (EP) and experimental periodontitis plus ethanol administration (EP+EtOH). On day 14 th , periodontitis was induced by ligatures that were placed around the lower first molars. On day 28 th , the animals were euthanized and mandibles were submitted to stereomicroscopy for exposed root area analysis and micro-computed tomography (micro-CT) for the evaluation of alveolar bone loss and microstructural parameters. Results The results revealed that ligature-induced alveolar bone loss is aggravated by ethanol binge drinking compared to controls (1.06 ± 0.10 vs 0.77 ± 0.04; p<0.0001). In addition, binge drinking per se altered the alveolar bone quality and density demonstrating a reduction in trabecular thickness, trabecular number parameter and bone density percentual. Periodontal disorder plus ethanol binge drinking group also demonstrated reduction of the quality of bone measured by trabecular thickness. Conclusions In conclusion, intense and episodic ethanol intake decreased alveolar bone quality in all microstructural parameters analyzed which may be considered a modifying factor of periodontitis, intensifying the already installed disease.

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Section: Discussionsupporting

confidence: 92%

Ethanol binge drinking exposure affects alveolar bone quality and aggravates bone loss in experimentally-induced periodontitis

et al. 2020

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“…According to Piemontese et al [ 37 ], intracortical remodelling is associated with increased cortical porosity and formation of secondary osteons that exhibit histologic hallmarks of remodelling activity in mice. Increased intracortical remodelling was also recorded in our previous researches in mice subacutely exposed to AA [ 6 ] and in Et-fed mice after 8 weeks [ 4 ].…”

Section: Discussionsupporting

confidence: 62%

“…Simultaneous consumption of Et and AA-rich food is widespread among humans. Detrimental effects of AA and Et on various organ systems including the skeleton have been described in several studies [ 2 , 3 , 4 , 5 , 6 ]. Their toxicity contributed to differences in selected biochemical and morphological parameters of various organs.…”

Section: Introductionmentioning

confidence: 99%

“…In the cortical bone, increased porosity and decreased values were established for relative bone volume and bone mineral density (BMD). In the trabecular bone, lower relative bone volume, trabecular number, trabecular thickness, and bone surface were determined [ 4 ]. In the study by Broulik et al [ 2 ], femoral bones of alcohol-fed rats (7.6 g of 95% Et/kg bw daily for 3 months) were characterised by a reduction in BMD, mechanical strength, cortical bone thickness, as well as in Ca and phosphate content.…”

Section: Introductionmentioning

confidence: 99%

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Antagonistic Impact of Acrylamide and Ethanol on Biochemical and Morphological Parameters Consistent with Bone Health in Mice

Martiniaková

Sarocka

Kováčová

et al. 2020

Animals

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The aim of present study was to verify antagonistic effect of acrylamide (AA) and ethanol (Et) on bone quality parameters. Adult mice (n = 20) were segregated into four groups following 2 weeks administration of toxins: group E1, which received AA (20 mg/kg body weight daily); group E2, which received 15% Et (1.7 g 100% Et/kg body weight daily); group E12, which received simultaneously both toxins; and a control group. An insignificant impact of individual applications of AA, Et or their simultaneous supplementation on the total body weight of mice and the length and weight of their femoral bones was identified. In group E1, higher levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), a decreased level of glutathione (GSH) and elevated endocortical bone remodelling were determined. A significantly lower relative volume of cortical bone, bone mineral density (BMD), elevated endocortical bone remodelling and cortical porosity, higher levels of ALT, AST, lower values for total proteins (TP), GSH, alkaline phosphatase (ALP), calcium, and phosphorus were recorded in group E2. In the mice from group E12, the highest endocortical bone remodelling, decreased values for BMD, TP, GSH and ALP and increased levels of ALT and AST were found. Our findings confirmed the antagonistic impact of AA and Et at doses used in this study on biochemical and morphological parameters consistent with bone health in an animal model.

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“…In our previous studies, single acrylamide and/or ethanol administration in the diet negatively affected murine bone microstructure after 48 h and/or 4 remodeling cycles, respectively [15, 16]. Here we report the interactive effects of both toxins on bone microstructure of mice after one remodeling cycle (2 weeks).…”

Section: Introductionmentioning

confidence: 56%

Single and simultaneous effects of acrylamide and ethanol on bone microstructure of mice after one remodeling cycle

Sarocka

Kováčová

Omelka

et al. 2019

BMC Pharmacol Toxicol

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Background This study aimed to examine femoral bone microstructure of mice after single and simultaneous administration to acrylamide and ethanol since both substances are often consumed separately and/or together by humans. Interactive effects of these toxins were analysed after one remodeling cycle. Methods Twenty clinically healthy adult mice were randomly divided into four groups following 2 weeks administration of toxins: A group - mice were fed with acrylamide (40 mg/kg bw); E group - mice were ethanol-fed (15% ethanol); AE group - mice were simultaneously fed with both toxins, and a C group – control (without acrylamide and/or ethanol supplementation). Generally, 2D and 3D imaging methods were used to determine cortical and trabecular bone tissues microstructure. Biochemical analyses of plasma parameters were also realized using commercially available ELISA tests and spectrophotometrically. Results Single and simultaneous exposure to acrylamide and ethanol affected only cortical bone microstructure. No significant changes in trabecular bone morphometry were detected among all groups. In mice from the A group, increased endocortical remodeling associated with a higher level of serum calcium and vasoconstriction of primary osteon’s vascular canals (POVC) were identified. On the contrary, increased cortical porosity consistent with a decreased relative bone volume, bone mineral density (BMD) and lower levels of alkaline phosphatase (ALP), glutathione (GSH), calcium in plasma and also with vasodilation of POVC were observed in the E group. In the AE group, the highest density of secondary osteons associated with a lower BMD and decreased levels of ALP, GSH were documented. The parameters of POVC and Haversian canals approximated to the C group. In addition, single and simultaneous exposure to both toxins caused liver disease consistent with a higher values of alanine aminotransferase (ALT), aspartate aminotransferase (AST) in plasma of all experimental groups. Conclusions Single administration to acrylamide and ethanol had negative effects on cortical bone structure of mice after one remodeling cycle. However, we identified possible antagonistic impact of these toxins on the structure of the cortical bone.

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Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol

Cited by 10 publications

References 47 publications

Ethanol binge drinking exposure affects alveolar bone quality and aggravates bone loss in experimentally-induced periodontitis

Ethanol binge drinking exposure affects alveolar bone quality and aggravates bone loss in experimentally-induced periodontitis

Antagonistic Impact of Acrylamide and Ethanol on Biochemical and Morphological Parameters Consistent with Bone Health in Mice

Single and simultaneous effects of acrylamide and ethanol on bone microstructure of mice after one remodeling cycle

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