Changes in the plasma erythropoietin level in rats following fasting, ageing, and anaemia

Naeshiro, Ichiro; Yoshioka, Mitsuaki; Chatani, Fumio; Sato, Shuzo

doi:10.1007/bf02642497

Cited by 8 publications

(7 citation statements)

References 18 publications

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“…The plasma erythropoietin level is known to be high in infant rats 24,25 . Naeshiro et al reported that overnight fasting influenced the erythropoietin level more severely in male F344 rats aged 6 weeks than those aged 10 weeks 24 . The large number of erythroblastic cells in infant rats gradually decreases 26,27 .…”

Section: Discussionmentioning

confidence: 99%

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Importance of Starting Age for Myelotoxicity Study in Dietary Restricted Rats

et al. 2009

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The aim of this study was to prove our hypothesis that adult rats with lowering of body weight gain, rats at 12 weeks of age as an example, are suitable for evaluation of myelotoxicity. Age-related differences between young rats (6-week-old study) and adult rats (12-week-old study) were analyzed in hematological examination values. The data of the young rats were reprinted from our previous report (Miyata et al., 2009) since our hypothesis was verified by comparison with that previous report. Several experimental groups were defined for the 12-week-old study as well as for the 6-week-old study; these included 5-fluorouracil (5-FU) treated groups receiving 12, 15 and 18 mg/kg/day (FU12, FU15 and FU18), pair-feeding groups (R12, R15 and R18 receiving the same amount of food as in the FU12, FU15 and FU18 groups, respectively) and a nontreated control group. Numerous hematologic and bone marrow parameters in the 5-FU treated groups were comparable to those in the corresponding pair-feeding groups in both age studies. Generally, the influences of undernutrition were more apparent in the young rats than in the adult rats. Histopathological examinations showed a decrease in hematopoiesis in the bone marrow in the 5-FU treated and pair-feeding groups. No apparent differences were observed in the decreased hematopoiesis between the 5-FU treated and pair-feeding groups in the 6-week-old study, but a difference between these groups was noted in the 12-week-old study; decreased hematopoiesis was more frequently noted in the 5-FU treated groups. These facts suggest that adult rats are more suitable than young rats for evaluation of 5-FU-induced myelotoxicity.

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Section: Discussionmentioning

confidence: 99%

“…The influence of undernutrition on erythroblastic changes cannot be disregarded. The plasma erythropoietin level is known to be high in infant rats 24,25 . Naeshiro et al reported that overnight fasting influenced the erythropoietin level more severely in male F344 rats aged 6 weeks than those aged 10 weeks 24 .…”

Section: Discussionmentioning

confidence: 99%

Importance of Starting Age for Myelotoxicity Study in Dietary Restricted Rats

et al. 2009

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“…We previously reported that the influence of undernutrition on erythroblastic changes cannot be disregarded in 14-day toxicity studies 10 , 11 . Moreover, the susceptibility of plasma erythropoietin levels to feeding conditions 16 originates from the decrease in the dietary protein content 17 – 19 . We considered that undernutrition, especially a reduction in dietary protein, might also result in a decrease in blood reticulocytes and marrow erythroid cells.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Short-term Myelotoxicity Study in Dietary Reduced Rats

et al. 2010

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This study attempted to prove our hypothesis that a short-term toxicity study, using a 4-day dosing regimen as an example, is suitable for evaluating myelotoxicity in rats. We compared the hematological, bone marrow cytological and histopathological results of 5-fluorouracil (5-FU) treated and pair-feeding groups after a 4-day administration period. Several experimental groups were defined for this 4-day study as well as for our previously reported 14-day study (Miyata et al., 2009); these included 5-FU treated groups receiving 12, 15 and 18 mg/kg/day (FU12, FU15 and FU18), pair-feeding groups (R12, R15 and R18 receiving the same amount of food as the FU12, FU15 and FU18 groups, respectively) and a nontreated control group. Although severe reductions in body weight gain and food consumption were reported in the 14-day study, only slight reductions were observed in the 4-day study. In the 4-day study, a decrease in blood reticulocytes and a decreasing trend of marrow erythroid cells were only observed in the FU18 group, and no effects were observed in the pair-feeding groups. The erythroblastic changes observed in this 4-day study were thought to reflect the direct influence of 5-FU administration. Since concerns regarding the influence of secondary changes related to undernutrition were minimized in the 4-day study, it was thought to clarify the direct influence of 5-FU administration on erythroblastic cells. Thus, a 4-day study protocol might be helpful for distinguishing secondary changes related to undernutrition.

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“…Phenylhydrazine induces many changes at the cellular level [14]. For example, it can modulate immune reactions [22], induce compensatory erythropoietin increase [13], vascular dysfunction, haemodynamic disturbance, a decrease in mean arterial pressure and hind limb vascular resistance [23], uncompensated respiratory alkalosis, increased arterial CO 2 tension and acidosis [24,25], skin and eye irritation [26]. Phenylhydrazine may also express genotoxic activity [27].…”

Section: Discussionmentioning

confidence: 99%

New Haemolytic Potential Assay on an Alternative Insect Model

Berger

2009

Basic Clin Pharma Tox

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Abstract:A new screening test of a haemolytic potential is presented on Spodoptera larvae in vivo. The assay was evaluated using phenylhydrazine, the well-known xenobiotic which induces haemolysis in mammals. Haemocyte disintegration causes changes in total haemocyte counts and differentials. It is shown that only qualitative evaluation of panoptically stained haemolymph smears is sufficient, fast and cheap to detect haemolytic potential. The presented assay also prevents unneeded, non-ethical use of vertebrates.

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Changes in the plasma erythropoietin level in rats following fasting, ageing, and anaemia

Cited by 8 publications

References 18 publications

Importance of Starting Age for Myelotoxicity Study in Dietary Restricted Rats

Importance of Starting Age for Myelotoxicity Study in Dietary Restricted Rats

Evaluation of Short-term Myelotoxicity Study in Dietary Reduced Rats

New Haemolytic Potential Assay on an Alternative Insect Model

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