Changes in the properties of mouse tongue muscle fibres before and after weaning

Maejima, Masayo; Abe, Shinichi; Sakiyama, Kazuo; Agematsu, Hiroko; Hashimoto, Masatsugu; Tamatsu, Yuichi; Ide, Yoshinobu

doi:10.1016/j.archoralbio.2005.03.007

Cited by 26 publications

(22 citation statements)

References 13 publications

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“…The functional roles of these four isoforms have been clarified. Therefore, the functions of muscles can be elucidated by determining the composition of these four isoforms (1,5,6,10,16,19,23,28) (Table 1). In this study, we examined MyHC-perinatal to determine speed of differentiation of myoblasts, and MyHC-2b, 2d, 2a and 1 to ascertain muscle cell characteristics.…”

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confidence: 99%

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

et al. 2007

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In vivo studies have shown that changes in the characteristics of skeletal muscle fiber are determined by type of exercise or training. These earlier studies on mechanical stimulation, however, have all employed stimulation applied at a constant intensity, and no studies appear to have investigated change with variation of intensity of stimulation. In this study, we investigated the characteristics and differentiation of myoblasts stretched at different rates. Myoblasts were stimulated at 3 different rates, and the numbers of cells and nuclei on days 1, 3, and 5 were compared. The myosin heavy chain (MyHC) mRNA expression level was also compared. We investigated expression of MyHC-perinatal to determine speed of differentiation of myoblasts, and expression of MyHC2b, 2d, and 2a to ascertain muscle cell characteristics. Counting cells and nuclei of myoblasts revealed clear promotion of differentiation with stretching. With rapid stretching, expression of MyHC-perinatal was high at first, but then showed a decrease. In terms of effect on muscle fiber characteristics, MyHC-2b, MyHC-2d, and MyHC-2a were high with rapid, medium, and slow stretching, respectively. This indicated that myoblast differentiation was promoted regardless of difference in stretching speed, with the myoblasts acquiring the muscle-fiber characteristics appropriate to each rate of stretching.Exercise and training are known to bring about changes in the characteristics of skeletal muscle fibers (26,27). Numerous studies of the human (2,3,4,12,13,17,20,24) have shown that skeletal muscle fiber characteristics change depending on the mode of exercise. Anaerobic exercise facilitates increases in fast-twitch muscle, while aerobic exercise facilitates increases in slow-twitch muscle. Studies using rats have reported that long-term low-intensity exercise increases slow-twitch muscle and decreases fast-twitch muscle (9,15). In this manner, in vivo studies in the rat and the human have shown that exercise changes muscle fiber characteristics. From the perspective of stress, severing a nerve controlling skeletal muscle not only decreases muscle weight, but also changes muscle fiber characteristics (7,14). Studies in which muscle blood flow was regulated using devices such as a tourniquet have shown that even low-intensity training caused marked muscle hypertrophy and increased muscle force (25). However, few in vitro studies have been conducted to support and clarify these findings. In vitro studies have attempted to ascertain the effects of exercise and training by applying different stimuli. The first study (18) removed cells from rat hindlimb and soleus muscle and applied electrical stimuli via a culture solution to measure expression of myosin heavy chain (MyHC) isoforms. In the second study (11), osteoblasts were compressed using glass boards to ascertain morphological and bio-

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confidence: 99%

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

et al. 2007

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“…It has also been shown that the MyHC isoforms undergo reversible changes (Sartorius et al, 1998). Moreover, these reversible changes occur according to changes in oral motor function (Gojo et al, 2002;Doi et al, 2003;Shida et al, 2005;Maejima et al, 2005). This indicates that oral motor function and composition ratios for the isoforms are closely related.…”

Section: Discussionmentioning

confidence: 96%

“…The portion of the tongue from the anterior to the sulcus terminalis was excised according to the method of Maejima et al (Maejima et al, 2005). A 5.0 mm-long area extending 2.5 mm anterior and 2.5 mm posterior to the midpoint between the sulcus terminalis and apex was harvested.…”

Section: Methodsmentioning

confidence: 99%

Myosin heavy chain composition of tongue muscle in microphthalmic (mi/mi) mice before and after weaning

Yanagisawa

Abe

Agematsu

et al. 2006

Annals of Anatomy - Anatomischer Anzeiger

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running title: MyHC in tongue muscle of mi/mi mice 2 SummaryTo elucidate the effects of teeth on muscle fibers in tongue during the development process, we examined the expression of muscle contractile proteins and the genes for those proteins in normal mice and microphthalmic (mi/mi) mice with impaired tooth eruption. The mice were observed during the growth period, including weaning, which is when feeding movements undergo major changes.Expression of the myosin heavy chain (MyHC)-2a protein, whose contraction speed is relatively slow, disappeared after weaning in normal mice, while it remained in high concentrations even after weaning in mi/mi mice. The presence of MyHC-2a after weaning in mice with no tooth eruption was attributed to a compensation for lack of proper masticatory function and sucking-like movements, as MyHC-2a is necessary for these movements.

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“…Maejima et al and Yanagisawa et al examined the changes of the muscle fiber characteristics of the upper and lower halves of the adult mouse tongue muscle before and after weaning, and confirmed marked changes of isoforms 15,16) . Thus, marked functional changes before and after weaning, i.e., from suckling to chewing, were demonstrated to influence the characteristics of muscle fibers in the oral cavity.…”

Section: Introductionmentioning

confidence: 99%

“…The tongue muscle consists of fast muscle type 5,15,16) ; thus, MyHC-2b and MyHC-2a, which is considered to have the highest and lowest contractile rates among fast muscle type MyHC isoforms, respectively, were selected to investigate the muscle fiber characteristics 4) . HMGB1 was used as a marker to examine whether the carcinoma of tongue actually developed 18) .…”

Section: Introductionmentioning

confidence: 99%

Characteristics and Effects of Muscle Fibers surrounding Lingual Carcinoma

Sakiyama

Takizawa

Bando

et al. 2013

J. Hard Tissue Biology.

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It has been suggested that dysphagia caused by excision of carcinoma of the tongue may be affected by the hypofunction of the surrounding muscle tissues, as well as by tissue defects. However, there have been no reports concerning how muscle fiber characteristics change in the regions surrounding the lesion after excision of the carcinoma. In this study, we investigated myosin heavy chain (MyHC) isoforms at genetic levels in order to demonstrate the muscle fiber characteristics of the muscle tissues surrounding lingual carcinoma (the experimental group). High mobility group box 1 (HMGB1) was used as a marker to examine whether tongue cancer actually developed. MyHC-2a expression was high in the experimental group. Thus, the marked changes of muscle fiber characteristics, as seen in this study, were considered to be a biological reaction to changing muscle characteristics for regenerating or maintaining normal muscle tissues against the muscle atrophy caused by carcinoma.

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Changes in the properties of mouse tongue muscle fibres before and after weaning

Cited by 26 publications

References 13 publications

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

Myosin heavy chain composition of tongue muscle in microphthalmic (mi/mi) mice before and after weaning

Characteristics and Effects of Muscle Fibers surrounding Lingual Carcinoma

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