Changes in the TBARs content and superoxide dismutase, catalase and glutathione peroxidase activities in the lymphoid organs and skeletal muscles of adrenodemedullated rats

Pereira, Benedito; Costa-Rosa, L.F.B.P.; Bechara, Etelvino J.H.; Philip, Nisha; Curi, Rui

doi:10.1590/s0100-879x1998000600016

Cited by 29 publications

(21 citation statements)

References 28 publications

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“…The frozen liver samples from each fish were thawed and homogenized using a teflon-glass Potter Elvejhem tissue homogenizer (1 min, 100 rpm) with 2 volumes of ice-cold medium containing 50 mM imidazole, 150 mM sucrose, 50 mM KCl, 1 mM EDTA and 2 mM reduced gluthatione (adjusted to pH 6.8 with HCl) (Mommsen et al, 1980). The homogenates were centrifuged at 10,000 g for 15 min at 4 o C (Pereira et al, 1998). The supernatants were used for glutathione peroxidase (GSH-Px, E.C.…”

Section: Methodsmentioning

confidence: 99%

Oxidative stress biomarkers and aggressive behavior in fish exposed to aquatic cadmium contamination

et al. 2009

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The objective of this study was to investigate the possible link between cadmium exposure, hepatic markers of oxidative stress and aggressive behavior in Nile tilapia (Oreochromis niloticus). Fish were first exposed to 0.75 mg/L CdCl 2 for 15 days (12 isolated fish for each group) and afterward a behavioral test was performed. Fish from the control and cadmium-exposed groups were paired for 1 h (6 pairs of fish per group) for determination of aggressiveness parameters. Immediately after the behavioral test, the animals were sacrificed and the liver was used to determine biochemical parameters. Cadmium decreased aggression in Nile tilapia. Subordinate animals exposed to cadmium showed decreased glutathione peroxidase (GSH-Px) activity compared to dominant ones. No alterations were observed in selenium-dependent glutathione peroxidase Se-GSH-P and Cu-Zn superoxide dismutase activities, but total superoxide dismutase activity was increased in subordinate animals exposed to cadmium compared to subordinate control. Catalase activity was increased in cadmium-exposed fish. Lipoperoxide concentrations also increased in cadmium exposed fish indicating that cadmium toxicity may affect oxidative stress biomarkers in Nile tilapia. Social stress induced lipoperoxidation in Nile tilapia, and subordinate animals exposed to cadmium responded with lower activities of liver antioxidant enzymes compared to dominant fish. The present study shows that cadmium exposure is capable of inducing changes in the social status and oxidative stress parameters in this species.O objetivo deste estudo foi investigar a possível relação entre a exposição ao cádmio, os marcadores hepáticos de estresse oxidativo e o comportamento agressivo em tilápia do Nilo (Oreochromis niloticus). Os peixes foram primeiramente expostos à concentração de 0,75 mg/L de CdCl 2 durante 15 dias (12 peixes isolados em cada grupo), após o qual o teste de comportamento foi aplicado. Peixes do grupo controle e do grupo exposto ao cádmio foram pareados durante 1 hora (6 pares de peixes por grupo) para determinação de parâmetros de agressividade. Imediatamente após o teste comportamental, os animais foram sacrificados e o fígado foi coletado para as determinações bioquímicas. O cádmio diminuiu a agressão em tilápias do Nilo. Animais subordinados e expostos ao cádmio mostraram uma redução na atividade da enzima glutationa peroxidase (GSH-Px), quando comparados aos dominantes. Nenhuma alteração foi observada na atividade da glutationa peroxidase dependente de selênio (Se-GSH-P) e na superóxido dismutase Cu Zn, mas a atividade total da superóxido dismutase aumentou em animais subordinados e expostos ao cádmio, quando comparado ao controle subordinado. A atividade da catalase aumentou em peixes expostos ao cádmio. As concentrações de lipoperóxido aumentaram em peixes expostos ao cádmio, indicando que a toxicidade deste pode afetar os biomarcadores de estresse oxidativo em tilápias do Nilo. O estresse social induziu lipoperoxidação em tilápia do Nilo e animais subordinados e expo...

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Section: Methodsmentioning

confidence: 99%

Oxidative stress biomarkers and aggressive behavior in fish exposed to aquatic cadmium contamination

et al. 2009

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“…What is more, the sharp increase in the superoxide dismutase activity, without proper activation in catalase is cytotoxic. Catalase catalyzes the splitting of hydrogen peroxide with the formation of water and oxygen (Pereira et al, 1998;Bielenichev et al, 2002;. The change of catalase activity in the conditions of feeding bulls with food cadmium chloride is given in the table 2.…”

Section: Resultsmentioning

confidence: 99%

Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load

Gutyj¹,

Stybel²,

Darmohray³

et al. 2017

Ukr J Ecol

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The results of studies of the effect of Cadmium influence on the state of the enzyme and nonenzyme system of antioxidant protection of young cattle organism, namely on the activity of superoxide dismutase, catalase, glutathione peroxidase, level of reduced glutathione, Selenium, vitamins A and E. It was determined that the feeding of young bulls with cadmium chloride in a dose of 0.04 mg / kg of body weight contributed to a decrease both, enzyme and non enzyme level of the system of antioxidant protection (superoxide dismutase by 22%, catalase -by 12%, renewed glutathione by 11%, glutathione peroxidase by 22 %, Selenium -by 18%, vitamin A -by 23%, vitamin E -by 27%,). The lowest level of the antioxidant protection system in the blood of young cattle was set at the sixteenth and twenty-fourth day of the experiment, which is due to the increased activation of lipoperoxidation processes and a disturbance of the balance between the activity of the antioxidant system and the intensity of peroxidation of lipids. The toxic effect of Cadmium contributes to the change in the stationary concentrations of the radical metabolites O2, ˙OH, NO2, which, in their turn, initiate the processes of peroxide oxidation of lipids, indicating an increase in the level of diene conjugates and malonic dialdehyde. The highest level of intermediate and final products of peroxid oxidation of lipids in the blood of young cattle was determined on the thirtieth day of the experiment, while comparing with the control group, they increased by 31 and 27%, respectively.

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“…In support of this it is established that stress increases oxidative damage and affects the processes of DNA repair in cells [51,52]. On coherence between adrenaline and oxidative stress pointed Pereira et al [53]. Their fi ndings pointed that adrenaline regulates the activities of catalase and glutathione peroxidase, but the exact mechanism remains to be determined.…”

Section: Discussionmentioning

confidence: 95%

The Investigation of DNA Damage Induced by Adrenaline in Human Lymphocytes in Vitro/Ispitivanja Oštećenja DNK Izazvanih Adrenalinom U Limfocitima Čoveka in Vitro

et al. 2014

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Adrenaline is a neurotransmitter and hormone that plays an important role in physiological regulatory mechanisms. The objective of this study was to assess primary DNA damage in isolated human lymphocytes exposed to adrenaline using the in vitro comet assay. Dose-response of human lymphocytes was determined at concentration range of adrenaline from 0.01 μM to 300 μM for various treatment times (1h, 2h, 4h and 24h). The obtained results showed that adrenaline induced DNA damage at concentration range from 5 μM to 300 μM after 1h, 2h and 4h of treatment. The slightest DNA damage was observed after 24 h of adrenaline treatment -only the highest concentrations of adrenaline (150 μM and 300 μM) caused increased level of DNA damage. In order to evaluate the potential contribution of reactive oxygen species (ROS) in adrenaline-induced DNA damage we used antioxidants catalase (100 IU/mL and 500 IU/mL) and quercetin (100 μM and 500 μM). Co-treatment of lymphocytes with adrenaline (300 μM) and antioxidants for 1 h, signifi cantly reduced the quantity of DNA in the comet tails. Therefore, it can be concluded that adrenaline exhibits genotoxic effects mainly through induction of reactive oxygen species and that some of the DNA damage is repaired during the fi rst four hours following the treatment with adrenaline.

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Changes in the TBARs content and superoxide dismutase, catalase and glutathione peroxidase activities in the lymphoid organs and skeletal muscles of adrenodemedullated rats

Cited by 29 publications

References 28 publications

Oxidative stress biomarkers and aggressive behavior in fish exposed to aquatic cadmium contamination

Oxidative stress biomarkers and aggressive behavior in fish exposed to aquatic cadmium contamination

Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load

The Investigation of DNA Damage Induced by Adrenaline in Human Lymphocytes in Vitro/Ispitivanja Oštećenja DNK Izazvanih Adrenalinom U Limfocitima Čoveka in Vitro

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