Changes in the volume of Sertoli cells during the cycle of the seminiferous epithelium in the rat

Bugge, H. P.; Plöen, L.

doi:10.1530/jrf.0.0760039

Cited by 25 publications

(15 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It occasionally was necessary to exclude portions of a tubule in instances where folds were present or where key areas of the montage were missing. This method, suggested by Bugge and Ploen ( 1986), insures proportional sampling of the seminiferous tubule components. Our recent findings of V, of Sertoli cell nuclei (expressed a s percentages) obtained from the pie-shaped wedges of the tubule (2.06 * 0.28) and those obtained from the whole tubule (2.10 * 0.04) profile (Sinha Hikim et al, 1989b) indicate that pieshaped wedges provide a n unbiased sampling of seminiferous tubules.…”

Section: Stzc Of the Sertoli Cellmentioning

confidence: 99%

A comparative study in twelve mammalian species of volume densities, volumes, and numerical densities of selected testis components, emphasizing those related to the sertoli cell

et al. 1990

View full text Add to dashboard Cite

Morphometric studies were performed on 12 mammalian species (degu, dog, guinea pig, hamster, human, monkey, mouse, opossum, rabbit, rat, stallion, and woodchuck) to determine volume density percentage (Vv%), volume (V), and numerical density (Nv) of seminiferous tubule components, especially those related to the Sertoli cell, and to make species comparisons. For most species, measurements were taken both from stages where elongate spermatids were deeply embedded within the Sertoli cell and from stages near sperm release where elongate spermatids were in shallow crypts within the Sertoli cell. Montages, prepared from electron micrographs, were used to determine Vv% of Sertoli cell components in seminiferous tubules. Excluding the tubular lumen, the Sertoli cell occupied from a high of 43.1% (woodchuck) to a low of 14.0% (mouse) of the tubular epithelium. There was a strong negative correlation (r = -0.83; P less than 0.005) of volume occupancy of Sertoli cells with sperm production. Nuclear volume, as determined by serial reconstruction using serial thick sections, ranged from a high of 848.4 microns 3 (opossum) to a low of 273.8 microns 3 (degu). There was no correlation (r = 0.02) of nuclear volume with volume occupancy (Vv%) in the tubule. Sertoli cell volume was determined by point-counting morphometry at the electron-microscope level as the product of the nuclear size and points determined over the entire cell divided by points over the nucleus. Sertoli cell V ranged from 2,035.3 microns 3 (degu) to 7,011.6 microns 3 (opossum) and was highly correlated (r = 0.85; P less than 0.001) with nuclear size. However, there was no significant correlation between the Sertoli cell size (V) and volume occupancy (Vv%; r = 0.13) or sperm production (r = -0.21). Stereological estimates of the numerical density (Nv) of Sertoli cells ranged from a high of 101.9 x 10(6) (monkey) to a low of 24.9 x 10(6) (rabbit) cells per cm3 of testicular tissue. There was no correlation of numerical density of Sertoli cells with sperm production (r = 0.002). A negative correlation was, however, observed between the numerical density of the Sertoli cells and the Sertoli cell size (r = -0.79; P less than 0.002). Data from the present study are compared with those previously published. This is the first study to compare Sertoli cell morphological measurements using unbiased sampling techniques. Morphometric data are provided which will serve as a basis for other morphometric studies.

show abstract

Section: Stzc Of the Sertoli Cellmentioning

confidence: 99%

A comparative study in twelve mammalian species of volume densities, volumes, and numerical densities of selected testis components, emphasizing those related to the sertoli cell

et al. 1990

View full text Add to dashboard Cite

show abstract

“…A pie-shaped montage (Bugge and Ploen, 1986) of the selected round seminiferous tubule including the boundary tissue a t stage VII or VIII in each animal was used to determine the volume density of the myoid cell (V,) in the seminiferous tubule. The montage was equivalent to one-eighth of the entire seminiferous tubule.…”

Section: Numerical Densitymentioning

confidence: 99%

Golden hamster myoid cells during active and inactive states of spermatogenesis: Correlation of testosterone levels with structure

et al. 1990

View full text Add to dashboard Cite

Myoid cells were examined quantitatively in adult golden hamsters with active spermatogenesis and compared with hamsters in which the testes were regressed due to a modification in the light-dark cycle. A detailed morphometric study was undertaken utilizing animals previously examined. The cell-surface area and volumes of most organelles were not significantly different in animals which were gonadally active as compared with regressed animals. A slight, but significant, increase in nuclear volume (31%) and a slight, but significant, decrease (28%) in cell volume were recorded for regressed animals. The total volume of pinocytotic vesicles was increased dramatically (approximately threefold) in active animals in comparison with inactive animals (P less than 0.01), indicating that an increase in non-specific transport across the myoid cell is associated with spermatogenic activity. Intravascularly injected horseradish peroxidase was capable of entering pinocytotic vesicles in both active and inactive animals. Plasma luteinizing hormone (LH) as well as plasma and testicular testosterone levels were weakly (r = 0.64, 0.68, and 0.65, respectively), but significantly (P less than 0.05), correlated with cell size. Plasma and testicular testosterone were correlated with the total volume of pinocytotic vesicles (r = 0.74 and 0.68, respectively). The data indicate that although the rat myoid cell possesses receptors for testosterone, there are few structural manifestations of the hamster myoid cell that correlate well with testosterone levels. Thus, the hamster myoid cell differs from two other hormone-responsive somatic cells in the testis, the Sertoli cell and the Leydig cell, that show dramatic structural alterations with changes in gonadal activity and striking correlations of structural features with functional measures.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

“…Pie-shaped [9] montages made from negatives taken at 2400 times and enlarged to 5900 times original size of round or near-round tubular profiles were constructed from individual electron micrographs to provide proportional sampling of the seminiferous epithelium [10]. In brief, the testes of four adult male rats were perfused-fixed with glutaraldehyde and the tissue was embedded in Araldite.…”

Section: Methodsmentioning

confidence: 99%

Surface and Surface-to-Volume Relationships of the Sertoli Cell during the Cycle of the Seminiferous Epithelium in the Rat1

França

Ghosh

et al. 1993

Biology of Reproduction

View full text Add to dashboard Cite

The surface relationships of the Sertoli cell and the surface relationships of the Sertoli cell in comparison to the changing volumes of developing germ cells were studied using morphometric techniques at periods representing nine groupings of the fourteen defined periods in the cycle of the seminiferous epithelium of the adult rat. No cyclic variation in the total Sertoli plasma membrane surface area was noted. Cyclic variations were noted in the area of the Sertoli cell surface that faces the basal compartment germ cells, but not the basal lamina. No cyclic variations were noted in the amount of contact of the Sertoli cells with each other at the level of the Sertoli cell barrier. However, when areas in the adluminal compartment were studied, significantly less Sertoli-Sertoli contact was seen in stages V through VII than in other stages with the exception of stages II-IV. Surface contact of germ cells with Sertoli cells increased progressively as germ cells entered the intermediate compartment and progressed to late spermatids. However, a calculation of the surface-to-volume ratio showed that surface increases of the Sertoli cell in relation to the volume of germ cells were greatest in elongating spermatids past step 12 of spermiogenesis. The area in which Sertoli ectoplasmic specializations faced germ cells was determined throughout spermatogenesis, and these data demonstrated that the first appearance of ectoplasmic specialization was at the mid-pachytene phase. They also showed that stage VIII was a period when ectoplasmic specialization loss from the cell surface was evident. Less Sertoli ectoplasmic specialization face step 8 and step 19 spermatids than comparable germ cell types at other stages. In addition to Sertoli cell surface area changes during the cycle, volumes of individual germ cell types were determined for the first time. The data presented allow an objective understanding of the complex structure and relationships of the Sertoli cell and provide a basis for understanding functional changes and interpreting biochemical data.

show abstract

Changes in the volume of Sertoli cells during the cycle of the seminiferous epithelium in the rat

Abstract: Summary. Volume

Cited by 25 publications

References 4 publications

A comparative study in twelve mammalian species of volume densities, volumes, and numerical densities of selected testis components, emphasizing those related to the sertoli cell

A comparative study in twelve mammalian species of volume densities, volumes, and numerical densities of selected testis components, emphasizing those related to the sertoli cell

Golden hamster myoid cells during active and inactive states of spermatogenesis: Correlation of testosterone levels with structure

Surface and Surface-to-Volume Relationships of the Sertoli Cell during the Cycle of the Seminiferous Epithelium in the Rat1

Contact Info

Product

Resources

About