Changes in the wing shape and size in Drosophila melanogaster treated with food grade titanium dioxide nanoparticles (E171) – A multigenerational study

Abstract

Drosophila is among the most commonly use models for toxicity assessment of different types of nanoparticles. This study aims to examine the effects of a constant exposure to the low concentration of human food grade titanium dioxide nanoparticles (TiO 2 E171) on Drosophila melanogaster wing morphology over multiple generations. Subsequently, the Geometric Morphometrics Analysis was employed to examine possible changes in the wing shape and size of the treated flies. The treatment resulted in the diminishment but not a disruption in the sexual dimorphism in wings. Consequently, the female flies were clearly separated from the male flies by the differences in wing morphology as in the control group. A splitting by generations was overly similar within the control and the treatment, but it was slightly more pronounced in the treatment. However, the observed generational differences seemed mostly random between generations, irrespective of the treatment. Specifically, the treated groups displayed slightly higher splitting by generations in females than in males. Regardless of the generation, the results show a clear splitting by the differences in the wing shape between the treated flies and the flies from control. The mean value of centroid size, which refers to the wing size, of both female and male wings was smaller in the treatment when compared to the control. The overall effect of TiO 2 was to induce significant difference in Drosophila wing morphology but it did not alter the general wing morphology pattern. Therefore, the change in the wings occurred only within the normally allowed wing variation.

Essential Oils of Three Balkan Abies Species: Chemical Profiles, Antimicrobial Activity and Toxicity toward Artemia salina and Drosophila melanogaster

“…In this way, we aimed to assess the perspective of ethnopharmacological uses of fir EOs in healing respiratory infections. Finally, in order to assess toxicity toward crustaceans and insects, we have examined the impact of EOs on brine shrimp Artemia salina (Anostraca: Artemiidae) in an acute toxicity model as well as on fruit fly Drosophila melanogaster (Diptera: Drosophilidae) in a sub‐chronic test, as these are common model organisms in toxicology studies [12,13] …”

Pseudotsuga menziesii (Pinaceae): Volatile Profiles, Antimicrobial Activity and Toxicological Evaluation of Its Essential Oil

“…To the best of authors’ knowledge, these are the first results obtained against pathogenic clinical strains, isolated from patient's specimens. Finally, in order to evaluate crustacean toxicity, we have investigated the effect of DEO on brine shrimp ( Artemia salina , Artemiidae) in an acute toxicity model as well as insecticidal activity on fruit fly ( Drosophila melanogaster , Drosophilidae) in a subchronic test as frequently used animal models in toxicology studies [12–14] …”

5-Fluorouracil And Curcumin With Pectin Coating As a Treatment Regimen For Titanium Dioxide Induced Colon Cancer Model

“…The amount of black pigment in both healthy and ulcerative colitis children's Peyer's patches increased with age, and it was discovered in both cases to be food-grade TiO 2 . Similar deposits, which the researchers believe to be food-grade TiO 2 , were discovered in colon cancer, Crohn's disease, and Crohn's colitis without the disease [Cvetković et al 2020, Brand et al 2020]. TiO 2 was found in lymphoid tissue of patients with Crohn's disease, ulcerative colitis, and colonic carcinoma.…”