5-Fluorouracil And Curcumin With Pectin Coating As a Treatment Regimen For Titanium Dioxide Induced Colon Cancer Model

Abstract

Objective: Induction of colorectal cancer in Wister rats using titanium dioxide and dimethylhydrazine and treatment using the physical conjugate of 5-Fluorouracil and Curcumin, with a synergistic approach.Methods: Compatibility studies are evaluated by using FT-IR,Vero cell lines, and HCT-116 cell lines are used for evaluating the synergistic approach. This followed by induction using titanium dioxide and dimethylhydrazine in Wister rats and treatment using 5-Fluorouracil and Curcumin with pectin coating. Result: The samples were found to be compatible. The synergistic effect was obtained at 1:1, 1:2, 1:4, and 2:1 ratio, where 1:4 ratio shows a CI50 value of 0.896, selected further for the animal studies when studied in HCT 116 cell lines and found to be safe with Vero cell lines. Colorectal cancer was shown to be induced within 70 days of administration of titanium dioxide and dimethylhydrazine.1:4 ratio of 5-Fluorouracil and Curcumin (50:200) shows effective for the treatment of colorectal cancer within 28 days, proven using histopathology report, bodyweight analysis, and hematological reports.Conclusion: 5-Fluorouracil and curcumin (1:4) ratio works with a synergistic approach and was proven effective for the treatment of colorectal cancer induced bythe titanium dioxide and dimethylhydrazine.