Changes in tissue and plasma free amino acid concentrations after feeding in Atlantic cod

Lyndon, Alastair Robert; Davidson, Ian; Houlihan, D.F.

doi:10.1007/bf00004503

Cited by 53 publications

(49 citation statements)

References 26 publications

(34 reference statements)

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“…Although there were no data on the individual food consumption rates in these studies, Dabrowski (1982) suggested that individual variation in blood and plasma FAA concentrations were largely due to differences in the amount of food ingested. In contrast, other studies have reported that the FAA concentrations change little following feeding (Walton and Wilson 1986;Lyndon et al 1993); FAA concentrations in the white muscle and stomach were relative- Tryptophan and cysteine were not measured after acid hydrolysis.…”

Section: Introductionmentioning

confidence: 64%

Effect of feeding on the tissue free amino acid concentrations in rainbow trout (Oncorhynchus mykiss Walbaum)

Carter

Houlihan

et al. 1995

Fish Physiol Biochem

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This study investigates whether tissue free amino acid (FAA) pools in rainbow trout,Oncorhynchus mykiss (Walbaum), are altered following feeding and the relationships between the amount of food consumed and the FAA pool size. Trout were starved for 7 days to provide baseline data and then refed on day 8. Individual food intake was measured by radiography and the consumption of amino acids (AA) calculated from dietary protein consumption. Total FAA concentrations in the stomach, liver and white muscle were little changed at various times after the meal and this pattern was repeated for the majority of individual FAA. Overall, the most notable change was a reduction in essential FAA concentrations (principally in valine, leucine and isoleucine) in the white muscle following feeding. However, in the caeca total FAA, total essential FAA and a number of individual FAA were significantly elevated at 4, 9 and 15h following feeding. There were few significant correlations between dietary amino acid consumption and total tissue FAA and essential FAA concentration in the stomach, caecum and white muscle; correlations were stronger in the liver. In order to explain the relative constancy of total FAA concentrations in the tissues following food intake (which represents over 100% of the total FAA pool) a model is presented that quantifies the AA flux through the free pools and considers the role of protein turnover in regulating FAA pool size.

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Section: Introductionmentioning

confidence: 64%

Effect of feeding on the tissue free amino acid concentrations in rainbow trout (Oncorhynchus mykiss Walbaum)

Carter

Houlihan

et al. 1995

Fish Physiol Biochem

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“…Endogenously formed found both single and double peaks in uptake profiles for cod (Lyndon et al, 1993), but their data show great divergence for the same free AAs between the two sample sites, suggesting ambiguous results due to serially slaughtering a group of fish to obtain blood samples from the HPV and by cardiac puncture. Espe et al also used serial slaughter with Atlantic salmon (Salmo salar) and reported three uptake profiles using caudal puncture to obtain blood (Espe et al, 1993).…”

Section: Experimental Considerationsmentioning

confidence: 98%

“…In fact, it is estimated that 20-50% of the free AAs entering the blood never get past the liver (Hoerr et al, 1991;Hoerr et al,1993;Biolo et al, 1992;Matthews et al, 1993). Lyndon et al tried to estimate the preand post-hepatic free AA plasma levels by serial slaughter of cod (Gadus morhua) and sampling of blood from HPV and cardiac puncture immediately after death (Lyndon et al, 1993). Ash et al (Ash et al, 1998) sampled blood using a double DA and HPV cannulation technique ) from lightly sedated fish but made only one measurement 3·h postprandially.…”

Section: Introductionmentioning

confidence: 99%

Postprandial changes in plasma free amino acid levels obtained simultaneously from the hepatic portal vein and the dorsal aorta in rainbow trout (Oncorhynchus mykiss)

Karlsson

Eliason

Mydland

et al. 2006

Journal of Experimental Biology

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SUMMARY For the first time, changes in plasma concentrations of free amino acid(AA) and their metabolites were followed simultaneously in pre- and post-hepatic blood following a single meal in non-anaesthetized and free-swimming fish. Rainbow trout (Oncorhynchus mykiss), kept in 10°C water and fitted with cannulae in the hepatic portal vein (HPV) and the dorsal aorta (DA), were force-fed 1% of their body mass and blood samples were taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandially to follow the free AA profile. Almost all free AAs increased rapidly within the first 3 h and only a few free AAs did not change significantly over time. By 6 h, the total free AA concentration had peaked in blood taken from both the DA(7107±369 nmol ml-1) and HPV (9999±572 nmol ml-1). However, individual free AAs showed three main profiles beyond this time: for type I, a peak concentration occurred only at 6 h; for type II, there was a more gradual rise in concentration to a peak at 24 h; and for type III there were two peaks, at 6 h and 24 h. All free AAs returned to or were lower than baseline levels within 48 h, with the exception of threonine and proline. The total free AA concentrations were consistently higher (P<0.05) in the HPV than in the DA at 3 h, 6 h, 12 h and 24 h. Our data provide clear evidence that, during the first pass through the liver, hepatic modification altered individual free AA concentrations as indicated by variable ratios among the simultaneous blood samples. Furthermore, the elevation of ammonium and urea in the HPV indicates intestinal catabolism of ingested free AA before release into the HPV. In conclusion, the dual HPV and DA cannulation shows promise as a useful technique for qualitative and quantitative investigations of absorption and turnover of nutrients, especially if the measurements can be combined with reliable estimates of blood flow and labelled substances.

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“…Moreover, it was not determined in fingerling rainbow trout either (25). Lyndon et al (15) point out the omission of tryptophan content unexplained in the literature. This omission is probably due to the low levels of tryptophan in tissues.…”

Section: Resultsmentioning

confidence: 99%

“…Free amino acid content in various fish species is also differentiated (7,8,15,20). Free amino acid concentration also depends on growth stage, starving, spawning migration, seasonal variation, water temperature and hardness, the storage and processing conditions of fresh fish as raw material, as well as diet.…”

Section: Praca Oryginalnamentioning

confidence: 99%

Free amino acid content in muscle tissue of bighead carp and wels catfish

Pyz‐Łukasik¹,

Szpetnar²,

Paszkiewicz³

et al. 2016

Medycyna Weterynaryjna

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The present research was aimed at a comparative analysis of free amino acid (FAA) levels in the muscle tissue of herbivorous and predatory fish. The FAA concentration in the muscle tissue samples from bighead carp and wels catfish was determined by ion-exchange chromatography. The bighead carp muscle tissue, as compared to that of wels catfish, showed significantly higher concentrations of arginine, histidine, methionine, phenyloalanine, alanine, asparagine, serine, glycine, and taurine, but significantly lower levels of isoleucine, leucine, threonine, valine, glutamine, cystationine, β-alanine, ethanoloamine, as well as aspartic, glutamic, cysteic, α- and γ-aminobutyric acids. The muscle tissues of bighead carp and wels catfish did not differ significantly in the levels of cystine, lysine, tryptophan, tyrosine, α-aminoadipic acid, cytrulline, ornithine and 1-methyl-histidine. Proline was detected only in the wels catfish muscle. The results obtained have shown differences in free amino acid concentration in muscle tissue of examined fish. A differentiated natural diet induces changes in free amino acid content in fish tissues. Knowledge of levels of free amino acids which are precursors for biogenic amines facilitates setting the safety criteria for fish and fishery products from species other than those mentioned in the Commission Regulation No 2073/2005

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Changes in tissue and plasma free amino acid concentrations after feeding in Atlantic cod

Cited by 53 publications

References 26 publications

Effect of feeding on the tissue free amino acid concentrations in rainbow trout (Oncorhynchus mykiss Walbaum)

Effect of feeding on the tissue free amino acid concentrations in rainbow trout (Oncorhynchus mykiss Walbaum)

Postprandial changes in plasma free amino acid levels obtained simultaneously from the hepatic portal vein and the dorsal aorta in rainbow trout (Oncorhynchus mykiss)

Free amino acid content in muscle tissue of bighead carp and wels catfish

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