Changes in Turkey Spermatozoza Motility Parameters After Addition of Copper Sulphate in Vitro

Slanina, Tomáš; Miškeje, Michal; Petrovičová, Ida; Lukáč, Norbert; Massányi, Péter

doi:10.15414/jmbfs.2015.4.special2.98-100

Cited by 5 publications

(6 citation statements)

References 2 publications

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“…Our results corroborate the findings of previous studies, reporting reductions in sperm motility and sperm velocity parameters after exposure to Cd or Cu in humans (Mukhopadhyay et al, 2010), rabbit (Roychoudhury & Massanyi, 2008), turkey (Slanina, Miškeje, Petrovičová, Lukáč, & Massányi, 2015), common carp (Chyb, Sokolowska-Mikolajczyk, Kime, Socha, & Epler, 2001) and African catfish (Kime, Ebrahimi, Nysten, Roelants, & Ollevier, 1996). Mukhopadhyay et al (2010) found a significant decline in VCL and STR in a tobacco-exposed group, whereas a heavy metal-exposed group had a significant reduced VCL and ALH.…”

Section: Discussionsupporting

confidence: 92%

“…Alternatively, this almost instant effect of CdCl 2 can be explained by the fact that Cd is highly toxic for mammalian cells (Telišman et al, 2000). Copper on the other hand occurs naturally, is an essential trace element and plays a vital part of several enzymes, such as ferroxidases, cytochrome c oxidase and superoxide dismutase (Slanina et al, 2015;Van Niekerk & Van Niekerk, 1989).…”

Section: Discussionmentioning

confidence: 99%

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Quantitative assessment of heavy metal effects on sperm function using computer-aided sperm analysis and cytotoxicity assays

et al. 2018

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One known environmental risk factor impacting on human reproduction is heavy metal pollution. Although some metals (e.g., Cu, Se and Zn) have protective effects on the male reproductive system in low doses, heavy metals can accumulate to toxic levels and result in poor semen quality and decreased sperm function. We investigated the effect of CuSO and CdCl (10, 50, 100 and 250 µg/ml or 500 µg/ml) on human sperm motility and vitality by using computer-aided sperm analysis (CASA) and two cytotoxicity assays (WST-1 and XTT). Several sperm motility parameters were significantly reduced after 5 hr of exposure to the highest concentrations of CuSO (250 µg/ml) and CdCl (500 µg/ml). The WST-1 assay also revealed significantly lower absorbance values for 50, 100 and 250 µg/ml CuSO and for 500 µg/ml CdCl ; however, no significant effect was seen with XTT. The calculated average IC value was 50.31± 4.34 µg/ml for CuSO and 392.32 ±76.79 µg/ml for CdCl . The effects of these metals were confirmed with MgCl , a positive control. This study provides threshold concentrations for the harmful effect of CuSO and CdCl on human spermatozoa and recommends the use of WST-1 as vitality assay in future in vitro studies.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Quantitative assessment of heavy metal effects on sperm function using computer-aided sperm analysis and cytotoxicity assays

et al. 2018

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“…Motility was assessed at six time intervals: 0, 1, 2, 3, 4, and 5 h after spermatozoa collection and experiments were realized in six repetitions. The samples were evaluated doi: 10.17221/79/2017-CJAS using the CASA system (Computer Assisted Semen Analyser) with the Sperm Vision ® program (Minitube, Germany) equipped with a microscope Olympus BX 51 (Olympus Corp., Japan) to assess the spermatozoa motility (Massanyi et al 2008;Krockova et al 2012;Slanina et al 2015a). Each sample (10 μl) was placed into Makler Counting Chamber ® (depth 10 μm) (Sefi Medical Instruments Ltd., Germany).…”

Section: Methodsmentioning

confidence: 99%

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Slanina¹,

Miškeje²,

Tirpák³

et al. 2018

Czech J. Anim. Sci.

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Slanina T., Miškeje M., Tirpák F., Błaszczyk M., Stawarz R., Massányi P. (2018): Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility. Czech J. Anim. Sci., 63, 127-135.The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine -from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision ® and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87-9.90%) if compared to 5°C (2.12-4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.

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“…Similarly, Hardneck et al [33] also found a significant negative effect on sperm motility at the highest concentrations of these two metals (250 µg/mL and 500 µg/mL). In turkey spermatozoa, it was reported that after 30 min of exposure, CuSO 4 (12.5 µg/mL to 50 µg/mL) significantly lowered the percentage total and progressive motility, as well as the values for VCL, ALH, and BCF [63].…”

Section: The Effect Of Heavy Metals On Sperm Motilitymentioning

confidence: 97%

Effect of Copper Sulphate and Cadmium Chloride on Non-Human Primate Sperm Function In Vitro

Hardneck

Villiers

Maree

2021

IJERPH

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In order to address the large percentage of unexplained male infertility in humans, more detailed investigations using sperm functional tests are needed to identify possible causes for compromised fertility. Since many environmental and lifestyle factors might be contributing to infertility, future studies aiming to elucidate the effect of such factors on male fertility will need the use of appropriate research models. The current study aimed to assess the effects of two heavy metals, namely copper sulphate, and cadmium chloride, on non-human primate (NHP) sperm function in order to establish the possibility of using these primate species as models for reproductive studies. Our combined results indicated that the functionality of NHP spermatozoa is inhibited by the two heavy metals investigated. After in vitro exposure, detrimental effects, and significant lowered values (p < 0.05) were obtained for sperm motility, viability and vitality, acrosome intactness, and hyperactivation. These metals, at the tested higher concentrations, therefore, have the ability to impair sperm quality thereby affecting sperm fertilizing capability in both humans and NHPs.

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Changes in Turkey Spermatozoza Motility Parameters After Addition of Copper Sulphate in Vitro

Cited by 5 publications

References 2 publications

Quantitative assessment of heavy metal effects on sperm function using computer-aided sperm analysis and cytotoxicity assays

Quantitative assessment of heavy metal effects on sperm function using computer-aided sperm analysis and cytotoxicity assays

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Effect of Copper Sulphate and Cadmium Chloride on Non-Human Primate Sperm Function In Vitro

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