Changes occurred in the testes and DNA pattern of males wax moth (Galleria mellonella) first generation as a result of irradiation of their parents

Abstract

Nowadays, the sterile insect technique is broadly used as a pest control measure. Therefore, the present study was conducted to investigate the alteration occurred in testes and DNA pattern as an effect of inherited sterility. Full grown pupae of the wax moth, Galleria mellonella were irradiated with 80 and 160 Gy of γ irradiation. The size of the testes was decreased by increasing of γ irradiation dose. Also, the size of the testes was decreased in F 1 males comparing with the size of the testes of both the parents and the untreated control. The effects of γ rays on the DNA patterns of adult male parents and F 1 males showed alterations among the controls, the treated parents and F 1 individuals. Exposure to radiation caused very frequently the appearance of some extra bands and the deficiency of others in the arbitrary random amplified polymorphic DNA-polymerase chain reaction amplification patterns of the irradiated insects.

Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation

“…The appearance of extra bands and disappearance of others, as a result of irradiation, were recorded throughout the investigation causing the variations in the PCR pattern among the different samples although they are belonging to the same generation. The variations in the PCR patterns depend on the primer used, the dose of gamma irradiation given to the insect, the kind of the irradiation source and differ from the parent, F1 and F2 Rizk et al (2017) stated that the effects of γ rays on the DNA patterns of adult male parents and F1 males Galleria melonella showed alterations among the controls, the treated parents and F1 individuals. Exposure to radiation caused very frequently the appearance of some extra bands and the deficiency of others in the arbitrary random amplified polymorphic DNA-polymerase chain reaction amplification patterns of the irradiated insects also Zahran et al (2017) Full-grown male pupae of the peach or guava fruit fly, Bactrocera zonata were irradiated with sterilizing and substerilising doses of gamma radiation.…”

Impact of Gamma Radiation on Differernt Stages of Spilostethus pandurus (Scopoli, 1763): A Biochemical Perspective

“…The activities increase might be a result of the testis damage by gamma radiation. Abdel Baki and Al Khalaf (2011), Rizk et al (2017) announced presence of changes in testis size of irradiated Plodia interpunctella (Lepidoptera: Pyralidae) and Galleria mellonella (Lepidoptera: Pyralidae) respectively. Moreover, Kheirallah et al (2017) confirmed that ultrastructure deformation in spermiogenesis of the Brochymena sulcata (Hemiptera: Pentatomidae) were found in gamma-irradiated males.…”