Changes of apolipoprotein A-IV in the human neonate: evidence for different inductions of apolipoproteins A-IV and A-I in the postpartum period

Rapid in vivo transport and catabolism of human apolipoprotein A-IV-1 and slower catabolism of the apoA-IV-2 isoprotein.

“…After washing, the same purified anti-apoA-IV antibodies conjugated with horseradish peroxidase (HRP) and diluted 1:2,000 were added to the wells and incubated at room temperature for 1.5 h. After washing, HRP substrate was added, the reaction stopped in 20 min, and absorbance read at 450 nm by an automated plate reader (Bio-Tek Instruments, Inc., Winooski, VT). A secondary plasma standard was developed after multiple measurements using a validated apoA-IV electroimmunoassay, which was previously described (48). Plasma cholesterol and triglycerides were quantitated by automated enzyme techniques on a VPSS analyzer (Abbott Labs, North Chicago, IL).…”

Sex-specific effects of the glutamine/histidine polymorphism in apo A-IV on HDL metabolism.

“…47 Apo A-IV was quantified by electroimmunodiffusion and a rabbit anti-human apo A-IV antiserum. 48 The serum concentration of HDL containing apo A-I but not apo A-II (Lp A-I) was determined with a commercially available electroimmunodiffusion assay (Sebia).…”

apoA-IV tagged with the ER retention signal KDEL perturbs the intracellular trafficking and secretion of apoB

“…The secretion of apoA-IV into mesenteric lymph rapidly increases during fat absorption in parallel with lymph triglycerides (11). Plasma apoA-IV levels increase after fat feeding (11)(12)(13) and decrease during fasting (14). Moreover, plasma apoA-IV levels are correlated with dietary fat intake (15) and are depressed in digestive disorders that cause fat malabsorption (16).…”