Changes of carp F_oF₁-ATPase in association with temperature acclimation

Abstract

Previously we have shown, using two-dimensional electrophoresis, that mitochondrial ATP synthase (F(o)F(1)-ATPase) beta-subunit is the 55-kDa protein increased in cold-acclimated carp Cyprinus carpio (Kikuchi K, Itoi S, and Watabe S. Fisheries Sci 65: 629-636, 1999). To clarify the coordinate expression in various subunits of carp F(o)F(1)-ATPase with temperature acclimation, we examined the differences in mRNA levels of mitochondrial proteins encoded by both nuclear and mitochondrial genes in fast muscle of carp acclimated to 10 and 30 degrees C. The mRNA levels of nuclear genes per unit weight of total RNA were nearly twofold higher in the 10 degrees C- than 30 degrees C-acclimated carp. However, the transcripts of mitochondrial genes for the 10 degrees C-acclimated carp in terms of the same comparing unit were six to seven times as much as those for the 30 degrees C-acclimated carp. The F(o)F(1)-ATPase activities measured at 10, 25, and 30 degrees C were nearly twofold higher for the cold-acclimated fish than their warm-acclimated counterparts. Such quantitative and qualitative changes in carp F(o)F(1)-ATPase may contribute to extra ATP production required to compensate for energy balance at suboptimal temperatures.

Coping with cold: An integrative, multitissue analysis of the transcriptome of a poikilothermic vertebrate

“…Thus, our expression data suggest that the capacity for ATP synthesis through oxidative phosphorylation increases at reduced temperatures. This finding is consistent with observations that mitochondrial transcripts (20), as well as the number of mitochondria (21), increase in the skeletal muscle of some cooled fish. The data presented here may indicate that this response may extend beyond skeletal muscle to all tissues.…”

Invertebrates Mitochondrial Function and Energetic Challenges

“…This mitochondrial enzyme comprises a catalytic sector F 1 (composed by  3  3  subunits), and a transmembrane hydrophobic sector F O (composed of at least three subunits: a, b 2 and c 10-12 ), both linked by a central and a peripheral stalk (Mueller et al, 2004). As in other mitochondrial complexes, this enzyme includes subunits encoded in both the nuclear and mitochondrial genomes, in a tightly coordinated process to assemble this multimeric complex (Itoi et al, 2003;Muhlia-Almazan et al, 2008). During the oxidative phosphorylation process in mitochondria, the electron transport chain generates a proton gradient that is proposed to drive the rotation of Fo, a central rotor located in the inner mitochondrial membrane.…”

Dynamic changes in cardiac mitochondrial metabolism during warm acclimation in rainbow trout

“…Moreover, due to the complexity of the thermal acclimation process, the different components of mitochondrial metabolism affected by temperature are not clear, as they may depend on the time course and the intensity of the thermal exposure (Hazel, 1972(Hazel, , 1995Sidell, 1983;Blier and Guderley, 1993;St-Pierre et al, 1998;Guderley, 2004;Itoi et al, 2003;Kraffe et al, 2007;Iftikar et al, 2014;Jayasundara et al, 2015). For example, in isolated mitochondria from the red muscle of rainbow trout, Oncorhynchus mykiss, a temperature increase from 5 to 9°C over 2 days (Bouchard and Guderley, 2003) or from 5 to 15°C over 3 days (Kraffe et al, 2007) did not significantly change rates of pyruvate oxidation (state 3 and state 4; oxygen consumption in presence and absence of ADP, respectively, when pyruvate is provided to the mitochondria).…”