Changes of NF-κB, p53, Bcl-2 and caspase in apoptosis induced by JTE-522 in human gastric adenocarcinoma cell line AGS cells: role of reactive oxygen species

Li, Hongliang; Chen, Dandan; Li, Xiaohong; Zhang, Haiwei; Lu, Yan-qing; Chun-ling, YE; Ren, Xian-Da

doi:10.3748/wjg.v8.i3.431

Cited by 48 publications

(19 citation statements)

References 34 publications

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“…Interestingly, the mutated gene product appears to play an active role in tumor progression [39]. ROS has strongly been correlated with p53 mediated apoptosis [40]. Cox-2 has been reported to regulate the expression of p53 and in turn is regulated by NF-kB that is well known for modulation of inflammatory molecules [41].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of tea polyphenols by targeting cyclooxygenase-2 through regulation of nuclear factor kappa B, Akt and p53 in rat mammary tumors

et al. 2009

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Breast cancer has become the second leading cause of cancer-related deaths worldwide. The control of this disease can be achieved through chemoprevention, which refers to the consumption of synthetic or naturally occurring agents to block, reverse, or delay the process of tumor development. Tea (Camellia sinensis), the most widely consumed beverage, has shown promises in the field of cancer chemoprevention. Inhibition of tumorigenesis by green or black tea polyphenols has been demonstrated in various in vitro and in vivo models. Here, we examined the inhibitory effect of green tea polyphenol (GTP) and black tea polyphenol (BTP) on the development of mammary tumors-induced by 7, 12-dimethylbenz (a) anthracene (DMBA) in female, Wistar rats. 13% and 33% of animals developed tumors in GTP and BTP supplemented groups, respectively. Both GTP and BTP are effective in significantly inhibiting the cumulative number of mammary tumors (by~92% and 77%, respectively) and in reducing their growth. Mechanistically, we investigated the effects of GTP and BTP on the components of cell signaling pathways, connecting biomolecules involved in cancer development. GTP and BTP supplementation as a sole source of drinking solution leads to scavenging of reactive oxygen species (ROS) (by~72% and 69%, respectively) by inhibiting cyclooxygenase-2 (Cox-2) and inactivation of phosphorylated forms of nuclear factorkappa B (NF-kB) and Akt. Altogether, the study suggests that both cultivars of tea, i.e. green and black, have antitumorigenic potential against DMBA-induced mammary tumorigenesis in Wistar rats. Further studies such as large and long term cohort studies and clinical trials are warranted.

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Section: Discussionmentioning

confidence: 99%

Inhibitory effects of tea polyphenols by targeting cyclooxygenase-2 through regulation of nuclear factor kappa B, Akt and p53 in rat mammary tumors

et al. 2009

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“…These fi ndings indicate that VLPs from AGSV-infected Vero cells make a specifi c reaction and possess the same antigens as those AGSV from the AGS cell line. Bar d 100 nm line reveals multinuclear giant cell formation, 21 inclusion body-like legion,4,7,29 and an IFN response defi ciency,13,14 and carries genes and proteins of SV5 26. In this study, we confi rmed the formation of multinuclear giant cells in the AGS cell line and detected extracellular VLPs on the surface of AGS cells.…”

supporting

confidence: 60%

Morphology and infectivity of virus that persistently caused infection in an AGS cell line

Ooi

Daikoku

et al. 2011

Med Mol Morphol

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A recent report has indicated that proteins and genes of simian virus 5 (SV5) are detected in a human gastric adenocarcinoma (AGS) cell line, which is widely provided for oncology, immunology, and microbiology research. However, the production of infective virions has not been determined in this cell line. In this study, the morphology and infectivity of the virus particles of the AGS cell line were studied by light and electron microscopy and virus transmission assay. The virus particles were approximately 176.0 ± 41.1 nm in diameter. The particles possessed projections 8-12 nm long on the surface and contained a nucleocapsid determined to be 13-18 nm in width and less than 1,000 nm in length. The virus was transmissible to the Vero cell line, induced multinuclear giant cell formation, and reproduced the same shape of antigenic virions. In this study, the persistently infected virus in the AGS cell line was determined to be infective and form reproducible virions, and a new morphological feature of SV5 was determined.

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“…It has been found that cells of different cancers and even different types of cells of a cancer exhibit significantly different sensitivity to TRAIL [14][15][16][17] . Nuclear transcript factor (NF-κB), survivin and Bcl-2 are apoptosis inhibitors and play a key role in the mechanism of anti-apoptosis of tumors [18][19][20][21][22][23][24][25][26][27][28][29] . If the activity of these factors is suppressed, tumor cells can undergo apoptosis and stop growing [30,31] .…”

Section: Introductionmentioning

confidence: 99%

Effect of NF-κB, survivin, Bcl-2 and Caspase3 on apoptosis of gastric cancer cells induced by tumor necrosis factor related apoptosis inducing ligand

Yang¹,

Fang²,

Wang³

et al. 2004

WJG

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AIM:To study the effect of NF-κB, survivin, Bcl-2 and Caspase3 on tumor necrosis factors related apoptosis inducing ligand (TRAIL) induced apoptosis of gastric cancer cells. METHODS:Gastric cancer cells of SGC-7901, MKN28, MKN45 and AGS lines were cultured in PRMI-1640 medium and the apoptosis rates of the cells of 4 lines were observed after treatment of tumor necrosis factors related apoptosis inducing ligand (TRAIL) with a flow cytometer. The expression of NF-κB, survivin, Bcl-2 and Caspase3 in gastric cancer cells of 4 lines was analyzed with Western blot. RESULTS:After the gastric cancer cells were exposed to TRAIL 300 ng/ml for 24 hours, the apoptosis rate was 36.05%, 20.27%, 16.50% and 11.80% in MKN28, MKN45, AGS and SGC-7901cells respectively. Western blot revealed that the expressions of NF-κB and survivin were lower in MKN28 cells than in MKN45, AGS and SGC-7901 cells. In contrast, the expression of Caspase3 was higher in MKN28 cells than in MKN45, AGS and SGC-7901 cells. CONCLUSION:There is a selectivity of TRAIL potency to induce apoptosis in gastric cancer cells of different cell lines. The anticancer potency of TRAIL is associated with the decreased expression of NF-κB and survivin and increased expression of Caspase3 of gastric cancer cells.Yang LQ, Fang DC, Wang RQ, Yang SM. Effect of NF-κB, survivin, Bcl-2 and Caspase3 on apoptosis of gastric cancer cells induced by tumor necrosis factor related apoptosis inducing ligand. World

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Changes of NF-κB, p53, Bcl-2 and caspase in apoptosis induced by JTE-522 in human gastric adenocarcinoma cell line AGS cells: role of reactive oxygen species

Abstract: These findings suggest that reactive oxygen species, NF-kB, p53, bcl-2 and caspase-3 may play an important role in the induction of apoptosis in AGS cells after treatment with JTE-522.

Cited by 48 publications

References 34 publications

Inhibitory effects of tea polyphenols by targeting cyclooxygenase-2 through regulation of nuclear factor kappa B, Akt and p53 in rat mammary tumors

Inhibitory effects of tea polyphenols by targeting cyclooxygenase-2 through regulation of nuclear factor kappa B, Akt and p53 in rat mammary tumors

Morphology and infectivity of virus that persistently caused infection in an AGS cell line

Effect of NF-κB, survivin, Bcl-2 and Caspase3 on apoptosis of gastric cancer cells induced by tumor necrosis factor related apoptosis inducing ligand

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