Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients

Jarzembowski, Tomasz; Daca, Agnieszka; Witkowski, Jacek M.; Rutkowski, Bolesław; Gołębiewska, Justyna; Dębska-Ślizień, Alicja

doi:10.1155/2013/687156

Cited by 9 publications

(8 citation statements)

References 11 publications

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“…Thus, ampicillin MICs were correlated with PBP5 expression, consistent with those of Montealegre et al (21). According to our results, it is possible that pbp5 variants in various isolates confer different PBP5 affinity and as a result, posttranslational modification of PBP5 or different binding to ampicillin may occur (24).…”

Section: Discussionsupporting

confidence: 91%

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Amino acid substitution mutations and mRNA expression levels of the pbp5 gene in clinical Enterococcus faecium isolates conferring high level ampicillin resistance

Darehkordi

Saffari

Mollaei

et al. 2019

APMIS

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Darehkordi H, Saffari F, Mollaei HR, Ahmadrajabi R. Amino acid substitution mutations and mRNA expression levels of the pbp5 gene in clinical Enterococcus faecium isolates conferring high level ampicillin resistance. APMIS 2019; 127: 115-122.In this study, clinical ampicillin-resistant Enterococcus faecium isolates with minimum inhibitory concentrations (MICs) for ampicillin in the ranges from 128 to ˃512 lg/mL (n = 17) and two ampicillin-susceptible isolates (MIC 1 lg/mL) were investigated. No b-lactamase production was detected in these isolates. Alterations in the C-terminal part of pbp5 and levels of pbp5 mRNA expression were investigated by sequencing and quantitative real-time qRT-PCR, respectively. Sequencing analysis revealed five different pbp5 alleles (A to E) having differences in 18 amino acid positions spanning from residue 426 to 642. Allele A (V-462 ? A, H-470 ? Q, M-485 ? A, N-496 ? K, A-499 ? T, E-525 ? D, N-546 ? T, A-558 ? T, G-582 ? S, E-629 ? V, K-632 ? Q, and P-642 ? L) was the most frequent allele. The presence of just two susceptible isolates in allele E suggests a possible correlation between amino acid patterns and MIC, even if there is no discernible correlation with specific single amino acid differences. Also, these were the only isolates that showed much lower expression of class B penicillin-binding protein 5 (PBP5) compared to isolates with MIC of 128 or greater. Thus, ampicillin MICs were correlated with PBP5 expression.

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Section: Discussionsupporting

confidence: 91%

“…. According to our results, it is possible that pbp 5 variants in various isolates confer different PBP5 affinity and as a result, posttranslational modification of PBP5 or different binding to ampicillin may occur .…”

Section: Discussionmentioning

confidence: 54%

Amino acid substitution mutations and mRNA expression levels of the pbp5 gene in clinical Enterococcus faecium isolates conferring high level ampicillin resistance

Darehkordi

Saffari

Mollaei

et al. 2019

APMIS

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“…In enterococcal strains isolated from RTx patients, the expression of the PBP5 gene was higher than in commensal strains. As cyclosporine seemed to promote higher expression of PBP5 than tacrolimus, -lactam antibiotics may be more effective when tacrolimus-based immunosuppression protocols are implemented [22].…”

Section: Multidrug-resistant Bacteriamentioning

confidence: 99%

Urinary Tract Infections in Renal Transplant Recipients

Gołębiewska¹,

Dębska‐Ślizień²

2018

Urinary Tract Infection - The Result of the Strength of the Pathogen, or the Weakness of the Host

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Renal transplantation (RTx) is the treatment-of-choice for a significant number of patients with end-stage renal disease. Despite recent accomplishments, both surgical and medical complications still exist. Urinary tract infection (UTI) is the most common infectious complication after RTx, while asymptomatic bacteriuria is the most common manifestation of bacteriuria. UTI can impair graft function, potentially reducing graft and patient survival.The aetiology changes with time after RTx. The epidemiology of most of these infections is also changing with resistant organisms being isolated more often than in the past. Several factors increase the risk of infection in RTx patients, and the presence of multiple risk factors in the same patient is not uncommon. These include immunosuppression, urinary flow impairment (most often caused by stenosis or strictures at the vesicoureteral junction, benign prostate hypertrophy or vesicoureteral reflux), and treatment-related factors such as the use of catheters and double-J stents. Early diagnosis and effective treatment are key elements in salvaging both the allograft and the patient. This chapter reviews the definitions, epidemiology, microbiology, screening, clinical manifestations, diagnosis, impact on renal allograft function, evaluation after diagnosis, treatment, prevention including long-term prophylaxis, and the unique challenges of diagnosing and managing recurrent bacterial UTIs in a RTx care setting.

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“…They were treated with tacrolimus, in contrast to patients with other end-stage renal failures. That could result in an increase of gene expression as was described by authors previously in the context of PBP5 (penicillin binding protein 5) gene expression [25]. It may lead to selection of commensal Enterococcus strains with higher ASA gene expression.…”

Section: Resultsmentioning

confidence: 81%

Prophages in Enterococcal Isolates from Renal Transplant Recipients: Renal Failure Etiologies Promote Selection of Strains

Daca

Jarzembowski

Witkowski

et al. 2014

BioMed Research International

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Infections caused by commensal bacteria may be fatal for the patients under immunosuppressive therapy. This results also from difficulty in identification of high risk strains. Enterococcal infections are increasingly frequent but despite many studies on virulence traits, the difference between commensal and pathogenic strains remains unclear. Prophages are newly described as important elements in competition between strains during colonization, as well as pathogenicity of the strains. Here we evaluate a difference in presence of pp4, pp1, and pp7 prophages and ASA (aggregation substance) gene expression in enterococcal isolates from renal transplant recipients (RTx) with different etiology of the end-stage renal failure. Prophages sequence was screened by PCR in strains of Enterococcus faecalis isolated from urine and feces of 19 RTx hospitalized at Medical University of Gdansk and 18 healthy volunteers. FLOW-FISH method with use of linear locked nucleic acid (LNA) probe was used to assess the ASA gene expression. Additionally, ability of biofilm formation was screened by crystal violet staining method. Presence of prophages was more frequent in fecal isolates from immunocompromised patients than in isolates from healthy volunteers. Additionally, both composition of prophages and ASA gene expression were related to the etiology of renal disease.

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Changes of PBP5 Gene Expression in Enterococcal Isolates from Renal Transplantation Recipients

Cited by 9 publications

References 11 publications

Amino acid substitution mutations and mRNA expression levels of the pbp5 gene in clinical Enterococcus faecium isolates conferring high level ampicillin resistance

Amino acid substitution mutations and mRNA expression levels of the pbp5 gene in clinical Enterococcus faecium isolates conferring high level ampicillin resistance

Urinary Tract Infections in Renal Transplant Recipients

Prophages in Enterococcal Isolates from Renal Transplant Recipients: Renal Failure Etiologies Promote Selection of Strains

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