A prototype for the remover of cherry tomato calyxes was designed and manufactured. The tap remover was designed and manufactured considering the conveyor transport speed, brush length and clearance, and diameter. These were adjusted in three levels to determine the optimal design factor. Performance tests were conducted using Icon 513, a circular-shaped cherry tomato variety, and Minimaru, a jujube-shaped cherry tomato variety. Conveyor transport speeds were set at 210, 280, and 350 mm/s; brush lengths at 70, 80, and 90 mm; brush clearances at 20, 22, and 24 mm; and brush diameters at 0.8, 1.0, and 1.2 mm. The two varieties showed a similar damage rate during calyx removal. However, Minimaru showed a higher calyx removal rate than Icon 513, indicating that it is most suitable for the calyx removal mechanization process.