Changes of reliability and efficiency of micronucleus bioassay in Vicia faba after exposure to metal contamination for several generations

Duan, Changqun; Hu, Bin; Guo, Tao; Luo, Mingbo; Xu, Xiangtao; Xu, Chunju; Wen, Chuanhao; Ling, Meng; Wang, Huanxiao

doi:10.1016/s0098-8472(00)00058-7

Cited by 17 publications

(5 citation statements)

References 17 publications

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“…The assay is easy to conduct, inexpensive, rapid, and sensitive in detecting both clastogenic and aneugenic effects in order to assess the genotoxicity of the contaminants (Duan et al, 2000;Hajjouji et al, 2007;Marcato-Romain et al, 2009).…”

Section: Micronucleus With Vicia Fabamentioning

confidence: 99%

Potential genotoxicity and risk assessment of a chlorinated flame retardant, Dechlorane Plus

Dou

Jin

et al. 2015

Chemosphere

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Section: Micronucleus With Vicia Fabamentioning

confidence: 99%

Potential genotoxicity and risk assessment of a chlorinated flame retardant, Dechlorane Plus

Dou

Jin

et al. 2015

Chemosphere

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“…Plant assays are quite easy to conduct, inexpensive, rapid and good predictors of genotoxicity (Panda and Panda, 2002). In particular, the V. faba micronucleus test is a very sensitive and useful method for the detection of both clastogenic and aneugenic effects (Duan et al, 2000;El Hajjouji et al, 2007;Marcato-Romain et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Lead-induced genotoxicity to Vicia faba L. roots in relation with metal cell uptake and initial speciation

Shahid

Pinelli

Pourrut

et al. 2011

Ecotoxicology and Environmental Safety

228

102

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. Lead-induced genotoxicity to Vicia faba L.roots in relation with metal cell uptake and initial speciation. Ecotoxicology and Environmental Safety, Elsevier, 2011, vol. 74, pp. 78-84. <10.1016/j.ecoenv.2010 a b s t r a c tFormation of organometallic complexes in soil solution strongly influence metals phytoavailability. However, only few studies deal with the influence of metal speciation both on plant uptake and genotoxicity. In the present study, Vicia faba seedlings were exposed for 6 h in controlled hydroponic conditions to 5 mM of lead nitrate alone and chelated to varying degrees by different organic ligands.Ethylenediaminetetraacetic acid and citric acid were, respectively, chosen as models of humic substances and low weight organic acids present in natural soil solutions. Visual Minteq software was used to estimate free lead cations concentration and ultimately to design the experimental layout. For all experimental conditions, both micronucleus test and measure of lead uptake by plants were finally performed. Chelation of Pb by EDTA, a strong chelator, dose-dependently increased the uptake in V. faba roots while its genotoxicity was significantly reduced, suggesting a protective role of EDTA. A weak correlation was observed between total lead concentration absorbed by roots and genotoxicity (r 2 ¼0.65). In contrast, a strong relationship (r 2 ¼0.93) exists between Pb 2 + concentration in exposure media and genotoxicity in the experiment performed with EDTA. Citric acid induced labile organometallic complexes did not demonstrate any significant changes in lead genotoxicity or uptake. These results demonstrate that metal speciation knowledge could improve the interpretation of V. faba genotoxicity test performed to test soil quality.

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“…The micronuclei (MN) tests in the root tip of Vicia faba and in vitro cultured lymphocytes have been widely used in biomonitoring genotoxicity both in water and soils (Ma et al 1995;Minissi and Lombi 1997;Stopper and Müller 1997;Duan et al 2000;) These tests were recommended by Micronuclei Assay Expert Panel at the Second International Workshop on Genotoxicity Test Procedures as a standard test in genotoxicity research (Humpage et al 2000). Micronuclei are little structures containing chromatin but not connected with a cell nucleus.…”

Section: Introductionmentioning

confidence: 99%

Red cabbage anthocyanin extract alleviates copper-induced cytological disturbances in plant meristematic tissue and human lymphocytes

2009

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Red cabbage is a source of health beneficial substances with antioxidant and antigenotoxic properties. HPLC analysis specifying the content of the investigated extract indicated that mainly anthocyanins (ATH) were responsible for its abilities. Cytological research was conducted with two experimental models: plant tissues--meristematic cells of Vicia faba, and animal tissue elements--human lymphocytes. Positive influence of ATH extract on mitotic activity of Vicia cells exposed to Cu(2+) stress, and inhibitory effect of ATH on cytotoxic actions of Cu(2+) on lymphocytes were demonstrated. In all experimental series with ATH application in combinations with Cu(2+), mitotic index (MI) were higher than those obtained for only Cu(2+) stressed tissues. Preincubation in ATH before Cu(2+) stress had the best effect. Similarly, after ATH applications in all tested series decrease in frequency of micronuclei (MN) appearance was noticed in comparison with only Cu(2+) stressed material. In the case of Vicia cells ATH acted effectively even applied after Cu(2+) stress. It suggests that this ATH mixture not only prevents and limits but also heals the cytological injury caused by Cu(2+) stress.

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Changes of reliability and efficiency of micronucleus bioassay in Vicia faba after exposure to metal contamination for several generations

Cited by 17 publications

References 17 publications

Potential genotoxicity and risk assessment of a chlorinated flame retardant, Dechlorane Plus

Potential genotoxicity and risk assessment of a chlorinated flame retardant, Dechlorane Plus

Lead-induced genotoxicity to Vicia faba L. roots in relation with metal cell uptake and initial speciation

Red cabbage anthocyanin extract alleviates copper-induced cytological disturbances in plant meristematic tissue and human lymphocytes

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