Changes of Serum Antibody Activities to Various Dietary Antigens Related to Gluten Withdrawal or Challenge in Children with Coeliac Disease

Scott, Helge; J, Ek; Brandtzæg, Per

doi:10.1159/000233680

Cited by 31 publications

(18 citation statements)

References 7 publications

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“…In children with coeliac disease, gluten challenge is known to signi®cantly increase IgG and IgA levels to gluten, whereas gluten elimination decreases these (Scott et al, 1985;Lindberg et al, 1985;Wauters et al, 1991). In healthy subjects a chronic increase of dietary antigens does not affect food antibody concentrations even if single large loads of dietary antigens may lead to formation of food antibodies as a physiological response (Gallagher et al, 1983).…”

Section: Discussionmentioning

confidence: 99%

Milk hypersensitivity in young adults

et al. 1999

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Objective: To estimate the prevalence of milk hypersensitivity in Finnish adults. Design: Cross-sectional study. Subjects: Two hundred men and 206 women aged 27 y randomly recruited from the population register in southwestern Finland. Interventions: The subjects were interviewed about their dairy product consumption, abdominal discomfort after dairy product intake and lactose intolerance. From serum samples, serum reactivity to milk protein and milk-speci®c IgG 1 , IgG 2 , IgG 3 and IgA were measured. Results: About 20% of the subjects reported abdominal discomfort after dairy product intake, whereas only 6.4% had been diagnosed to have lactose intolerance. The amount of milk consumed correlated well with the serum assay results in subjects reporting abdominal discomfort but not in subjects who were free from these symptoms. Among subjects with no record of dairy product restriction or lactose intolerance, those experiencing abdominal discomfort after dairy product intake had signi®cantly higher serum reactivity to milk protein than those without such discomfort. The concentrations of serum milk-speci®c antibodies did not differ between these two groups. The prevalence of milk hypersensitivity in this population was estimated to be 3 ± 6%. Conclusions: Milk hypersensitivity may be as common in adults as in infants. The measurement of serum reactivity to milk protein may prove useful in screening milk hypersensitivity in subjects who have not restricted their dairy product consumption.

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Section: Discussionmentioning

confidence: 99%

Milk hypersensitivity in young adults

et al. 1999

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“…In previous studies of Scott et al [8,17] IgA antibodies to OA and BLG in untreated CD patients were regularly demon strated. We found low frequencies of IgA antibodies of both subclasses to these antigens.…”

Section: Discussionmentioning

confidence: 92%

“…IgA antibodies have in particular been investi gated in coeliac disease (CD), since measurement of serum IgA anti-gliadin antibodies represents a pro mising diagnostic tool as a marker of intestinal muco sal damage [6][7][8], In addition, serum levels of IgA an tibodies to other dietary antigens are often increased in CD [8], probably due to increased gut permeability [9-H].…”

Section: Introductionmentioning

confidence: 99%

ELISA Analysis of IgA Subclass Antibodies to Dietary Antigens

Kemp¹,

Husby²,

Larsen³

et al. 1988

Int Arch Allergy Immunol

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Enzyme immunoassays for the quantitation of IgA1 and IgA2 antibodies to dietary antigens were developed. Serum IgA1 antibodies to bovine serum albumin (BSA) were detectable in 2/30 healthy adults, in 3/26 healthy children, and at high levels in 8/11 children with coeliac disease, without relation to gluten exposure. IgA1 antibodies to ovalbumin (OA) and beta-lactoglobulin (BLG) at high titers were seen in one coeliac child but were otherwise low or absent. IgA2 antibodies to BSA were detectable in 28/48 healthy subjects and in 8/11 coeliac children. IgA2 antibodies to OA and BLG were measurable in a few samples from each group. IgA1 antibodies to the gluten component glycgli were found at low levels in 15/56 normal sera, and anti-glycgli antibodies of the IgA2 subclass in 14/48 sera from healthy persons, also at low levels. IgA1 anti-glycgli antibodies were measurable in 5/11 sera from CD patients on a gluten-free diet. Elevated levels of IgA1 anti-glycgli antibodies were detected in all sera from CD patients challenged with gluten, except in 1 patient with a markedly reduced serum IgA level. In contrast, the IgA2 anti-glycgli antibody levels were unaffected. Thus, increased levels of IgA antibodies to dietary protein antigens in childhood coeliac disease were observed only within the IgA1 isotype.

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“…IgA Abs are relatively specific for active CD, but they are occasionally detected in serum from patients with a variety of other intestinal disorders including Crohn's disease, cow's milk-sensitive enteropathy and intestinal infections with parasites [3][4][5][6]. When CD patients are treated with a gluten-free diet (GFD), IgA Abs titers fall rapidly, providing a criterion of ad herence to the GFD [7,8],…”

Section: Introductionmentioning

confidence: 99%

IgA Immune Response Patterns to Gliadin in Serum

Mascart‐Lemone¹,

Cadranel²,

Broeck³

et al. 1988

Int Arch Allergy Immunol

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The incidence, titer and molecular size of anti-gliadin (GL) IgA were analyzed in sera from children with celiac disease (CD, n = 66), hospitalized control children (n = 64) and children with other malabsorption disorders (n = 103). Anti-GL IgA was assessed by solid-phase radioimmunoassay, and its molecular size was analyzed by sucrose density gradient ultracentrifugation. The median anti-GL IgA titer was significantly higher in untreated CD than in the other groups, with some overlap. Whereas 57% of anti-GL IgA were polymeric IgA (pIgA) in acute CD, only 17% were pIgA in children with other malabsorption disorders. These anti-GL pIgA consisted mainly of dimers with in some children, a low proportion of anti-GL secretory IgA (SIgA). A large proportion of anti-GL pIgA was associated with high total serum IgA. After the onset of a gluten-free diet (GFD), anti-GL pIgA disappeared more quickly from serum than mlgA. During the gluten challenge test, anti-GL IgA comprised 20% pIgA when they reappeared in serum, but 59% pIgA at the end of the test. The presence of anti-GL pIgA in serum seems related to chronic exposure to gluten of CD patients with a flat intestinal mucosa. These serum pIgA antibodies could result from a spillover from the intestinal mucosal synthesis into the circulation.

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Changes of Serum Antibody Activities to Various Dietary Antigens Related to Gluten Withdrawal or Challenge in Children with Coeliac Disease

Cited by 31 publications

References 7 publications

Milk hypersensitivity in young adults

Milk hypersensitivity in young adults

ELISA Analysis of IgA Subclass Antibodies to Dietary Antigens

IgA Immune Response Patterns to Gliadin in Serum

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