Changing in the production of anticancer drugs (vinblastine and vincristine) in Catharanthus roseus (L.) G. Don by potassium and ascorbic acid treatments

Abstract

Catharanthus roseus seedling was treated with different concentrations (1.5, 3.16, 15, and 30 mmol) and forms (K<sub>2</sub>SO<sub>4</sub> and KNO<sub>3</sub>) of potassium (K<sup>+</sup>) via Hoagland’s nutrient solution. Ascorbic acid (AsA) was sprayed twice (plant days 68 and 78) with different concentrations (750 and 1 500 mg/L) on the leaves. Vinblastine, vincristine, tryptophan contents, D4H and DAT genes expression, peroxidase activity, and H<sub>2</sub>O<sub>2</sub> content of leaves were measured. Potassium in KNO<sub>3</sub> form increased vinblastine (60%) and vincristine (50%), compared to 30% and 20% using K<sub>2</sub>SO<sub>4</sub>. Vinblastine and vincristine inhibit microtubule assembly and ultimately metaphase-arrested caused by the polymerisation. The genes expression was higher 3 times in KNO<sub>3</sub> and 2.5 times in K<sub>2</sub>SO<sub>4</sub> in excess of K<sup>+</sup>. Foliar application of 750 mg/L AsA led to an increase in vinblastine (20%) and vincristine (16%). Both concentrations of AsA had the same additional effect on the expression of D4H and DAT about 30% and 60%, respectively, compared to the control plant. Tryptophan decreased 2.5 times in excess of K<sup>+</sup> and 35% due to the exterior of AsA. H<sub>2</sub>O<sub>2</sub> decreased while peroxidase activity increased along with AsA treatment. A positive interaction existed between the K<sup>+</sup> and AsA on the amount of vinblastine, vincristine, tryptophan, and gene expression.

Pharmacokinetics and antioxidant activity of dihydrocaffeic acid grafted chitosan nanomicelles loaded with chicoric acid in broilers

“…Subsequent intracellular transport involves different organelles to achieve exocytosis ( Yan et al, 2019 ), and the addition of various intracellular inhibitors helps explore this mechanism. Vinblastine, a microtubule inhibitor, disrupts microtubule protein polymerization, thus impeding microtubule structure synthesis ( Sahi et al, 2022 ). Chloroquine stabilizes lysosomal membranes, reducing lysosomal enzyme release and hindering lysosomal activity ( Fedele and Proud, 2020 ).…”

Computational analysis and expression profiling of potassium transport-related gene families in mango (Mangifera indica) indicate their role in stress response and fruit development

“…Five subgroups are used to classify the Shaker family: weak inward rectifying channels, inward rectifying channels with KAT-like characteristics, outward rectifying channels, inward rectifying channels with AKT-like characteristics, and the silent/regulatory subunit ( González et al., 2015 ). Moreover, the carrier-like KUP/HAK/KT family ( Azeem et al., 2022 ), HKT uniporters and symporters ( Sahi et al., 2022 ), and KEA antiporters ( Wang et al., 2017 ) also manifested their involvement in this mechanism. The KUP/HAK/KT family may regulate K + with great affinity ( Ye et al., 2013 ).…”