Changing paradigms in dermatology: confocal microscopy in clinical and surgical dermatology

González, Salvador; Swindells, Kirsty J.; Rajadhyaksha, Milind; Torres, Abel

doi:10.1016/j.clindermatol.2003.08.007

Cited by 94 publications

(114 citation statements)

References 25 publications

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“…Permite, ainda, a avaliação de processos dinâmicos na resposta a tratamentos: observou-se, com a MC, que, após alguns minutos do tratamento de hemangioma com pulse dye laser, o fluxo sanguí-neo dentro das lesões cessou e foi substituído por material amorfo. 41 …”

Section: Microscopia Confocalunclassified

Métodos objetivos para análise de estudos em dermatologia cosmética

Manela-Azulay

Cuzzi

Pinheiro³

et al. 2010

An. Bras. Dermatol.

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L Métodos objetivos para análise de estudos em dermatologia cosméticaObjective methods for analyzing outcomes in research studies on cosmetic dermatologyResumo: A Dermatologia Cosmética é uma área em constante crescimento, motivo pelo qual se faz necessária a utilização de métodos objetivos para validar os resultados dos estudos científicos. As técnicas mais empregadas na maioria desses trabalhos são: histopatologia, imunoistoquímica, morfometria, estereologia, fotografia digital, biometria, profilometria óptica e microscopia confocal. Esta revisão tem como objetivo trazer informações sobre os principais métodos utilizados como ferramenta de análise dos resultados obtidos, além de auxiliar o dermatologista a aguçar seu senso crítico em relação às publicações e apresentações que se utilizam de métodos subjetivos de avaliação. Palavras-chave: Dermatologia; Eficácia; Métodos Abstract: Cosmetic dermatology is a field of medicine that is in constant development; therefore, the use of objective methods for validating the findings of scientific studies is crucial. The most commonly used techniques in the majority of these studies include histopathology, immunohistochemistry, morphometry, stereology, digital photography, biometry, optical profilometry and confocal microscopy. The objective of this review was to provide an update on the principal methods used as tools for analyzing outcomes and also to provide the dermatologist with means of sharpening his/her critical judgement with respect to the publications and presentations that use subjective evaluation methods.

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Section: Microscopia Confocalunclassified

Métodos objetivos para análise de estudos em dermatologia cosmética

Manela-Azulay

Cuzzi

Pinheiro³

et al. 2010

An. Bras. Dermatol.

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“…A number of optical methods have been developed to monitor non-invasively different tissue components in the context of disease diagnosis and monitoring [25][26][27][28][29]. Such methods have only recently started to be exploited as tools for assessing different properties of the cell and matrix components of engineered tissues [30][31][32][33].…”

Section: Competing Interest Statementmentioning

confidence: 99%

“…A strong SHG (green) signal from collagen I fibers was collected in the forward direction through a 400nm bandpass filter; TPEF from the silk scaffold (red) was collected through a 455nm bandpass filter. (Bar =100 μm) [19,25,40] …”

Section: Supplementary Materialsmentioning

confidence: 99%

Non-invasive characterization of structure and morphology of silk fibroin biomaterials using non-linear microscopy

et al. 2008

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Designing biomaterial scaffolds remains a major challenge in tissue engineering. Key to this challenge is improved understanding of the relationships between the scaffold properties and its degradation kinetics, as well as the cell interactions and the promotion of new matrix deposition. Here we present the use of non-linear spectroscopic imaging as a non-invasive method to characterize not only morphological, but also structural aspects of silkworm silk fibroin-based biomaterials, relying entirely on endogenous optical contrast. We demonstrate that two photon excited fluorescence and second harmonic generation are sensitive to the hydration, overall β sheet content and molecular orientation of the sample. Thus, the functional content and high resolution afforded by these noninvasive approaches offer promise for identifying important connections between biomaterial design and functional engineered tissue development. The strategies described also have broader implications for understanding and tracking the remodeling of degradable biomaterials under dynamic conditions both in vitro and in vivo.

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“…In the past decade the interest in studying the propagation of polarized light in birefringent media has significantly increased [8][9]. Polarization-based theoretical methods, such as timeresolved [10] and Mie theory-based [11] Monte Carlo algorithms, and experimental techniques, such as polarization-sensitive optical coherence tomography (PS-OCT) [1][2][3][4] and cross-polarized confocal laser scanning microscopy (CPCLSM) [12][13] have advanced the understanding of birefringence in biological tissues. These theoretical and experimental tools were used to characterize a change in polarization of light due to birefringent properties of biological tissues.…”

Section: Introductionmentioning

confidence: 99%

Contrast improvement in scattered light confocal imaging of skin birefringent structures by depolarization detection

Varghese

Verhagen

Tai

et al. 2011

Journal of Biophotonics

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Journal of BIOPHOTONICSHere we describe a method for enhancing the contrast in imaging skin birefringent structures. The method relies on polarization-dependent optical properties and is implemented using cross polarized confocal microscopy. The experimental data obtained using ex-vivo and invivo measurements on human scalp hairs and human skin demonstrate a significant dependence of the change in polarization of light that interacted with the birefringent hair on the orientation of the incident polarization. The polarization dependent contrast, defined as the ratio of intensity measured for different orientations of the incident polarization when observed using cross polarized confocal microscopy furthermore depends on the hair type/degree of pigmentation and on the focusing depth inside the hair. No such dependence was observed for the upper skin layers, including the stratum corneum and epidermis. We propose a new method for enhancing the contrast between the skin and the birefringent hair by the use of cross polarized confocal microscopy combined with the variation of the polarization of the incoming light. Potential applications of this method include imaging of hairs for assessing the efficacy of hair removal methods and measurement of skin birefringence. The underestimation of the birefringence content resulting from the orientation related effects associated with the use of linearly polarized light for imaging tissues containing wavy birefringent structures could be minimized by this method.CPCLSM images obtained in-vivo with the direction of incident polarization at different angles to the orientation of the hair axis.

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Changing paradigms in dermatology: confocal microscopy in clinical and surgical dermatology

Cited by 94 publications

References 25 publications

Métodos objetivos para análise de estudos em dermatologia cosmética

Métodos objetivos para análise de estudos em dermatologia cosmética

Non-invasive characterization of structure and morphology of silk fibroin biomaterials using non-linear microscopy

Contrast improvement in scattered light confocal imaging of skin birefringent structures by depolarization detection

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