Channels and transporters for inorganic ions in plant mitochondria: Prediction and facts

Vothknecht, Ute C.; Szabó, Ildikò

doi:10.1016/j.mito.2020.05.007

Cited by 12 publications

(6 citation statements)

References 174 publications

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“…The Small Conductance Mechanosensitive Ion Channel (MscS) Family contains 11 members in Arabidopsis, out of which At4g00290 (mechanosensitive ion channel protein 1, mitochondrial) was both predicted and found, whereas At1g49260 was only predicted. It is possible that the mechanosensitive ion channel protein 1 can catalyze ATP-dependent K + channel activity by teaming up with one of the ABC transporters [ 46 ].…”

Section: The Different Transporter Classes and Familiesmentioning

confidence: 99%

Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria

et al. 2020

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To function as a metabolic hub, plant mitochondria have to exchange a wide variety of metabolic intermediates as well as inorganic ions with the cytosol. As identified by proteomic profiling or as predicted by MU-LOC, a newly developed bioinformatics tool, Arabidopsis thaliana mitochondria contain 128 or 143 different transporters, respectively. The largest group is the mitochondrial carrier family, which consists of symporters and antiporters catalyzing secondary active transport of organic acids, amino acids, and nucleotides across the inner mitochondrial membrane. An impressive 97% (58 out of 60) of all the known mitochondrial carrier family members in Arabidopsis have been experimentally identified in isolated mitochondria. In addition to many other secondary transporters, Arabidopsis mitochondria contain the ATP synthase transporters, the mitochondria protein translocase complexes (responsible for protein uptake across the outer and inner membrane), ATP-binding cassette (ABC) transporters, and a number of transporters and channels responsible for allowing water and inorganic ions to move across the inner membrane driven by their transmembrane electrochemical gradient. A few mitochondrial transporters are tissue-specific, development-specific, or stress-response specific, but this is a relatively unexplored area in proteomics that merits much more attention.

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Section: The Different Transporter Classes and Familiesmentioning

confidence: 99%

Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria

et al. 2020

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“… 1 While it is well established that this divalent cation can alter the fate of a plant cell, the mechanisms by which plant cells transport Ca 2+ are still under study. 2 Intracellular calcium buffering and storage play important roles in the homeostasis of this cation. Consequently, organellar calcium transport and sensing lays at the center of plant metabolism following multivariate physiological and pathological cues.…”

Section: Introductionmentioning

confidence: 99%

An Arabidopsis mutant line lacking the mitochondrial calcium transport regulator MICU shows an altered metabolite profile

Gutiérrez-Mireles,

Páez-Franco,

Rodríguez-Ruíz

et al. 2023

Plant Signaling & Behavior

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Plant metabolism is constantly changing and requires input signals for efficient regulation. The mitochondrial calcium uniporter (MCU) couples organellar and cytoplasmic calcium oscillations leading to oxidative metabolism regulation in a vast array of species. In Arabidopsis thaliana , genetic deletion of AtMICU leads to altered mitochondrial calcium handling and ultrastructure. Here we aimed to further assess the consequences upon genetic deletion of AtMICU. Our results confirm that AtMICU safeguards intracellular calcium transport associated with carbohydrate, amino acid, and phytol metabolism modifications. The implications of such alterations are discussed.

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“…A plethora of inner membrane transporting proteins has been described in the inner mitochondrial membrane [14]. These include anion-and cation-selective ion channels as well as transport proteins that affect membrane potential, such as ATP/ADP translocase [15,16]. The following potassium channels were identified in the inner mitochondrial membrane: mitochondrial ATP-sensitive potassium (mitoK ATP ) channels [17][18][19][20], mitochondrial large-conductance calcium-activated potassium (mitoBK Ca ) channels [21][22][23], mitochondrial intermediate-conductance calcium-activated potassium (mitoIK Ca ) channels [24,25], mitochondrial small-conductance calcium-activated potassium (mitoSK Ca ) channels [26,27], mitochondrial sodium-activated potassium (mitoSLO2) channels [28], mitochondrial voltage-regulated potassium (mitoKv) channels [29,30], and mitochondrial double-pore potassium (mitoTASK) channels [31,32] (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment

Walewska

Krajewska

Stefanowska

et al. 2022

IJMS

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In this paper, the techniques used to study the function of mitochondrial potassium channels are critically reviewed. The majority of these techniques have been known for many years as a result of research on plasma membrane ion channels. Hence, in this review, we focus on the critical evaluation of techniques used in the studies of mitochondrial potassium channels, describing their advantages and limitations. Functional analysis of mitochondrial potassium channels in comparison to that of plasmalemmal channels presents additional experimental challenges. The reliability of functional studies of mitochondrial potassium channels is often affected by the need to isolate mitochondria and by functional properties of mitochondria such as respiration, metabolic activity, swelling capacity, or high electrical potential. Three types of techniques are critically evaluated: electrophysiological techniques, potassium flux measurements, and biochemical techniques related to potassium flux measurements. Finally, new possible approaches to the study of the function of mitochondrial potassium channels are presented. We hope that this review will assist researchers in selecting reliable methods for studying, e.g., the effects of drugs on mitochondrial potassium channel function. Additionally, this review should aid in the critical evaluation of the results reported in various articles on mitochondrial potassium channels.

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Channels and transporters for inorganic ions in plant mitochondria: Prediction and facts

Cited by 12 publications

References 174 publications

Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria

Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria

An Arabidopsis mutant line lacking the mitochondrial calcium transport regulator MICU shows an altered metabolite profile

Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment

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