Chaperonin containing TCP-1 subunit 3 is critical for gastric cancer growth

Li, Lijuan; Zhang, Liansheng; Han, Ze‐Guang; He, Zhaohong; Chen, Hao; Li, Yumin

doi:10.18632/oncotarget.22838

Cited by 28 publications

(42 citation statements)

References 32 publications

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“…These proteins determine the central role of CCT in the proliferation of cancer cells. The expression of CCT3 in cancer tissue is higher than that in noncancerous tissue, and knockdown or suppression of the expression of CCT3 can inhibit the proliferation of cancer cells in many malignant carcinomas, such as hepatocellular carcinoma [9], gastric carcinoma [10], and papillary thyroid carcinoma [11]. In our study, we found that transduction with the lentiviral shRNA targeting CCT3 suppressed the mRNA and protein expression of CCT3 in the breast cancer cell lines HCC1937 and MDA-MB-231.…”

Section: Discussionsupporting

confidence: 50%

“…In gastric cancer, a higher level of CCT3 expression was detected in tumour tissues than in non-cancerous epithelial tissues. Knockdown of CCT3 inhibited the proliferation and survival of gastric cancer cells, and gene expression analysis showed that CCT3 knockdown was associated with down-regulation of mitogen-activated protein kinase 7, cell division cycle 42(cdc42), cyclin D3 and up-regulation of cyclin-dependent kinase 2 and 6 [10]. In papillary thyroid carcinoma, knockdown of CCT3 decreased the proliferation and cell cycle progression and induced the apoptosis of K1 cells [11].…”

Section: Introductionmentioning

confidence: 99%

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Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells

Wang

et al. 2020

Cancer Cell Int

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Background: CCT3 is a subunit of chaperonin-containing TCP-1 (CCT), which folds many proteins involved in cancer development and plays an important role in many cancers. However, the role of CCT3 in breast cancer is still unclear. Methods: CCT3 expression was knocked down by transfecting breast cancer cells with lentiviral shRNA. The proliferation of breast cancer cells (HCC1937 and MDA-MB-231) was detected by Celigo image cytometry and MTT assay, the migration of the cells was measured by Transwell analysis, cell cycle distribution and apoptosis was detected by flow cytometry, and changes in signal transduction proteins were detected by western blot analysis. Results: The expression of CCT3 was significantly suppressed by transduction with lentiviral shRNA; CCT3 knockdown significantly reduced the proliferation and metastasis ability of breast cancer cells (HCC 1937 and MDA-MB-231), increased the proportion of cells in S phase, and decreased the proportion of cells in G1 phase compared to those in shControl cells. There was no significant change in the number of cells in the G2/M phase. Apoptosis analysis showed that knockdown of CCT3 induced apoptosis in breast cancer cells. Western blot analysis showed that the expression of many signal transduction proteins was changed after suppression of CCT3. A rescue experiment showed that overexpression of NFκB-p65 rescued the cell proliferation and migration affected by CCT3 in breast cancer cells. Conclusion: CCT3 is closely related to the proliferation and migration of breast cancer and may be a novel therapeutic target.

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Section: Discussionsupporting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells

Wang

et al. 2020

Cancer Cell Int

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“…Moreover, a reduced synthesis of CCT6B mRNA was observed in hepatocellular carcinoma when compared to healthy tissue while the other components of TRiC complex were overexpressed [ 34 ]. The upregulation of several TRiC subunits were previously associated with high proliferative cancer rate [ 35 , 36 ]. Thus, it is possible that CCT6B overexpression in the non-seminomatous testicular cancer group may explain the higher invasiveness capacity of non-seminomatous TGCTs relative to seminomatous TGCTs.…”

Section: Discussionmentioning

confidence: 99%

Distinct Proteomic Profile of Spermatozoa from Men with Seminomatous and Non-Seminomatous Testicular Germ Cell Tumors

Selvam

Alves

Dias

et al. 2020

IJMS

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Testicular germ cell tumors (TGCTs) are predominant in young males (15–44 years). Seminomatous and non-seminomatous TGCTs account for about 98% of all TGCTs cases. In this study, we aimed to compare the sperm proteome of patients with seminomatous and non-seminomatous TGCTs to identify possible protein biomarkers that could help distinguish between them in a non-invasive manner. We analyzed semen samples from patients with seminomatous or non-seminomatous TGCTs (n = 15/group) that were cryopreserved before the start of cancer treatment. Quantitative proteomic analysis was conducted on pooled samples (n = 3/group) and a total of 258 differentially expressed proteins (DEPs) were identified. The overexpression of acrosin precursor (ACR) and chaperonin containing TCP1 subunit 6B (CCT6B) as well as the underexpression of S100 calcium-binding protein A9 (S100A9) in the spermatozoa of patients with non-seminomatous TGCTs were validated by western blotting conducted on individual samples (n = 6 for seminomatous group and n = 6 for non-seminomatous group). Our overall results suggest an association between the higher and faster invasiveness of non-seminomatous TGCTs and the altered protein expressions, providing important information for future studies.

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“…These proteins determine the central role of CCT in the proliferation of cancer cells. The expression of CCT3 in cancer tissue is higher than that in non-cancerous tissue, and knockdown or suppression of the expression of CCT3 can inhibit the proliferation of cancer cells in many malignant carcinomas, such as hepatocellular carcinoma [9] , gastric carcinoma [10] , and papillary thyroid carcinoma [11] . In our study, we found that transduction with the lentiviral shRNA targeting CCT3 suppressed the mRNA and protein expression of CCT3 in the breast cancer cell lines HCC1937 and MDA-MB-231.…”

Section: Discussionmentioning

confidence: 99%

“…In gastric cancer, a higher level of CCT3 expression was detected in tumour tissues than in non-cancerous epithelial tissues. Knockdown of CCT3 inhibited the proliferation and survival of gastric cancer cells [10] . In papillary thyroid carcinoma, knockdown of CCT3 decreased the proliferation and cell cycle progression and induced the apoptosis of K1 cells [11] .…”

Section: Introductionmentioning

confidence: 97%

Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells

Wang

et al. 2020

Preprint

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Background CCT3 is a subunit of chaperonin-containing TCP-1 (CCT), which folds many proteins involved in cancer development and plays an important role in many cancers. However, the role of CCT3 in breast cancer is still unclear. Methods CCT3 expression was knocked down by transfecting breast cancer cells with lentiviral shRNA. The proliferation of breast cancer cells (HCC1937 and MDA-MB-231) was detected by Celigo image cytometry and MTT assay, the migration of the cells was measured by Transwell analysis, cell cycle distribution and apoptosis was detected by flow cytometry, and changes in signal transduction proteins were detected by western blot analysis. Results The expression of CCT3 was significantly suppressed by transduction with lentiviral shRNA; CCT3 knockdown significantly reduced the proliferation and metastasis ability of breast cancer cells (HCC 1937 and MDA-MB-231), increased the proportion of cells in S phase, and decreased the proportion of cells in G1 phase compared to those in shControl cells. There was no significant change in the number of cells in the G2/M phase. Apoptosis analysis showed that knockdown of CCT3 induced apoptosis in breast cancer cells. Western blot analysis showed that the expression of many signal transduction proteins was changed after suppression of CCT3. Conclusion CCT3 is closely related to the proliferation and migration of breast cancer and may be a novel therapeutic target.

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Chaperonin containing TCP-1 subunit 3 is critical for gastric cancer growth

Cited by 28 publications

References 32 publications

Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells

Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells

Distinct Proteomic Profile of Spermatozoa from Men with Seminomatous and Non-Seminomatous Testicular Germ Cell Tumors

Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells

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