Distinct Proteomic Profile of Spermatozoa from Men with Seminomatous and Non-Seminomatous Testicular Germ Cell Tumors

Selvam, Manesh Kumar Panner; Alves, Marco G.; Dias, Tânia R.; Pushparaj, Peter Natesan; Agarwal, Ashok

doi:10.3390/ijms21144817

Cited by 5 publications

(3 citation statements)

References 44 publications

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“…An example is represented by the Arylsulfatase A, a protein implicated in gametes fusion, whose levels decreased after both slow-and rapid-freezing, suggesting that it could be used as a marker of sperm quality after cryopreservation [126,127]. Of note, it has been reported that oncological patients show already altered proteomic profiles before cancer treatments [128][129][130] and, therefore, at the moment of cryopreservation. In such a situation, eventual cryodamage to protein expression is added to already-existing damage.…”

Section: Damage To Mrnas Proteins and Metabolitesmentioning

confidence: 99%

Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects

Traini

Vignozzi

Baldi

et al. 2023

IJMS

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Cryopreservation is an expanding strategy to allow not only fertility preservation for individuals who need such procedures because of gonadotoxic treatments, active duty in dangerous occupations or social reasons and gamete donation for couples where conception is denied, but also for animal breeding and preservation of endangered animal species. Despite the improvement in semen cryopreservation techniques and the worldwide expansion of semen banks, damage to spermatozoa and the consequent impairment of its functions still remain unsolved problems, conditioning the choice of the technique in assisted reproduction procedures. Although many studies have attempted to find solutions to limit sperm damage following cryopreservation and identify possible markers of damage susceptibility, active research in this field is still required in order to optimize the process. Here, we review the available evidence regarding structural, molecular and functional damage occurring in cryopreserved human spermatozoa and the possible strategies to prevent it and optimize the procedures. Finally, we review the results on assisted reproduction technique (ARTs) outcomes following the use of cryopreserved spermatozoa.

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Section: Damage To Mrnas Proteins and Metabolitesmentioning

confidence: 99%

Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects

Traini

Vignozzi

Baldi

et al. 2023

IJMS

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“…There is an opportunity to apply these methodologies to serum investigations as well, as performed by Strenziok and collaborators [ 175 ], who through surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS) disclosed a cluster of proteins differentially present in TGCT patients compared to healthy subjects. In particular, such an approach could also be envisioned for seminal plasma [ 176 ], with a recent study already demonstrating distinct proteome of spermatozoa from patients with seminomas and non-seminomas of the testis [ 177 ].…”

Section: Future Directionsmentioning

confidence: 99%

Liquid Biopsies in the Clinical Management of Germ Cell Tumor Patients: State-of-the-Art and Future Directions

Lobo

Leão

Jerónimo

et al. 2021

IJMS

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Liquid biopsies constitute a minimally invasive means of managing cancer patients, entailing early diagnosis, follow-up and prediction of response to therapy. Their use in the germ cell tumor field is invaluable since diagnostic tissue biopsies (which are invasive) are often not performed, and therefore only a presumptive diagnosis can be made, confirmed upon examination of the surgical specimen. Herein, we provide an overall review of the current liquid biopsy-based biomarkers of this disease, including the classical, routinely used serum tumor markers—the promising microRNAs rapidly approaching the introduction into clinical practice—but also cell-free DNA markers (including DNA methylation) and circulating tumor cells. Finally, and importantly, we also explore novel strategies and challenges for liquid biopsy markers and methodologies, providing a critical view of the future directions for liquid biopsy tests in this field, highlighting gaps and unanswered questions.

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“…Portanto, a alteração dessas células é uma grande ameaça à fertilidade masculina (Selvam et al, 2020). Dois grupos principais dividem os tumores de células germinativas testiculares (TCGT), constituindo um grupo heterogêneo de tumores: os tumores do tipo pré-púberes, não derivados da neoplasia de células germinativas in situ (NCGIS), e os tumores do tipo pós-púberes, derivados do NCGIA, que ocorrem em homens jovens (seminomas e tumores de células germinativas não seminomatosos).…”

Section: Introductionunclassified

Tumor de células germinativas não seminomatoso

Caixeta¹,

Machado²,

Araújo³

et al. 2022

Braz. J. Hea. Rev.

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Introdução: o Tumor de Células Germinativas Não Seminomatoso (TCGNS) é responsável por 1% das neoplasias em homens, sendo mais invasivo e agressivo quando comparado ao subtipo Seminomatoso. É o câncer de testículo com maior índice de metástase para ossos, SNC, fígado e pulmões. Apresentação do caso: JCN, sexo masculino, 25 anos, internado em serviço terciário com quadro de dispneia aos mínimos esforços, hemoptise, perda de peso e dor torácica de início há 06 meses. Iniciada investigação de massa mediastinal encontrada após exames de imagem, sendo classificada como carcinoma embrionário não seminomatoso de origem mediastinal pela punção por agulha grossa. Discussão: os TCGNS frequentemente apresentam metástases para o trato gastrointestinal, podendo evoluir com abdome agudo. Os sítios de maior acometimento são duodeno e intestino delgado, sendo as manifestações: obstrução intestinal, volvo, intussuscepção e hemorragia. Sendo assim, deve-se atentar à possibilidade de tumor testicular como diagnóstico diferencial de abdome agudo. Conclusão: os Tumores de Células Germinativas representam 95% dos tumores testiculares, sendo mais comuns em homens entre 15 e 35 anos, sendo que os TCGNS possuem pico entre 20 e 24 anos. A hereditariedade é o principal fator de risco. Estes possuem dificuldade para diagnóstico precoce e tratamento, pois se manifestam como massa unilateral, indolor e de crescimento lento. Radiografia de tórax, TC ou ressonância magnética podem ser utilizadas para rastreamento de metástases. O tratamento se dá pela orquiectomia radical inguinal com vigilância pós cirúrgica e, se necessária, terapia adjuvante com quimioterapia ou radioterapia.

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Distinct Proteomic Profile of Spermatozoa from Men with Seminomatous and Non-Seminomatous Testicular Germ Cell Tumors

Cited by 5 publications

References 44 publications

Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects

Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects

Liquid Biopsies in the Clinical Management of Germ Cell Tumor Patients: State-of-the-Art and Future Directions

Tumor de células germinativas não seminomatoso

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