Chaperonin GroEL hydrolyses ortho-nitrophenyl β-galactoside

Abstract

We serendipitously found that chaperonin GroEL can hydrolyze ortho-nitrophenyl β-galactoside (ONPG), a well-known substrate of β-galactosidase enzyme. The ONPG hydrolysis by GroEL follows typical enzyme kinetics. Our experiments and molecular...

Visual monitoring of biocatalytic processes using small molecular fluorescent probes: strategies-mechanisms-applications

“…This reaction is used for the diagnosis and detection of tumours as well as the detection of senescent cells. 7–9…”