Chapter 129 The biochemistry of the f-elements

Duffield, John R.; Taylor, David; Williams, David R.

doi:10.1016/s0168-1273(05)80052-2

Cited by 11 publications

(13 citation statements)

References 74 publications

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“…While some have suggested that transferrin may carry Pu into cells 17 , extensive in vitro experiments have been taken to imply that plutonium-transferrin complexes are not taken into cells in a manner similar to the iron-transferrin complex 2,18–20 , presumably because Pu 4+ is a larger ion than Fe 3+ and strongly prefers higher coordination numbers. Instead, an ill-defined uptake pathway, which does not involve transferrin, has been invoked as the source of intracellular Pu 18,20 .…”

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confidence: 99%

An iron-dependent and transferrin-mediated cellular uptake pathway for plutonium

et al. 2011

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Plutonium is a toxic synthetic element with no natural biological function, but it is strongly retained by humans when ingested. Using small angle X-ray scattering, receptor binding assays, and synchrotron X-ray fluorescence microscopy we find that rat adrenal gland (PC12) cells can acquire plutonium in vitro through the major iron acquisition pathway, receptor-mediated endocytosis of the iron transport protein serum transferrin; however only one form of the plutonium-transferrin complex is active. Low-resolution solution models of plutonium-loaded transferrins derived from small angle scattering demonstrate that only transferrin with plutonium bound in the protein’s C-terminal lobe and iron bound in the N-lobe (PuCFeNTf) adopts the proper conformation for recognition by the transferrin receptor protein. Although the metal binding site in each lobe contains the same donors in the same configuration and both lobes are similar, the differences between transferrin’s two lobes act to restrict, but not eliminate, cellular Pu uptake.

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confidence: 99%

An iron-dependent and transferrin-mediated cellular uptake pathway for plutonium

et al. 2011

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“…Nevertheless, under in-vivo conditions, the exclusive occurrence of these species is unlikely. Regardless whether incorporation occurs via ingestion or inhalation or through the skin, heavy metals are absorbed into and transported by the bloodstream prior to their deposition in target organs (Ansoborlo et al, 2006;Duffield et al, 1994). There is agreement in literature that, in blood, trivalent lanthanides and actinides are mainly bound to proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Biological actinide research often concentrated on quantitative properties, like uptake, concentration, and retention (Duffield et al, 1994) as well as dose-dependent relationships between toxicity and intake to blood (Durbin, 2006). Nowadays, this is no longer sufficient, since the bioavailability, mobility, and accumulation as well as the toxicity of actinides is governed to a large extent by their speciation, too (Ansoborlo et al, 2006;Bresson et al, 2011Bresson et al, , 2013aMilgram et al, 2008a).…”

Section: Introductionmentioning

confidence: 99%

Interaction of Eu(III) with mammalian cells: Cytotoxicity, uptake, and speciation as a function of Eu(III) concentration and nutrient composition

Sachs

Heller

Weiß

et al. 2015

Toxicology in Vitro

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“…The binding constants of Pr(III), Ca(II) and Zn(II) with inorganic ligands were mostly cited from references (Ni, 1995;SillÈn et al, 1977;SillÈn et al, 1964;Rizkalla et al, 1994). And the binding constants of Pr(III), Ca(II) and Zn(II) with proteins were mainly cited from references (Duffield et al,1994;Xu, 1995). Since some of the binding constants were not determined at physiological conditions, Davies corrections were performed to generate new constants applicable to physiological conditions by the MINTEQA2 program.…”

Section: Model Of Human Interstitial Fluidmentioning

confidence: 99%